{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:33:11Z","timestamp":1791909191294,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T00:00:00Z","timestamp":1182384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2007,7,10]]},"abstract":"<jats:p>The Fe atom in the bimetallic active site of [NiFe]\u2010hydrogenases has one CO and two cyanide ligands. To determine their metabolic origin, [NiFe]\u2010hydrogenase\u20102 was isolated from <jats:italic>Escherichia coli<\/jats:italic> grown in the presence of <jats:sc>l<\/jats:sc>\u2010[ureido\u2010<jats:sup>13<\/jats:sup>C]citrulline, purified and analyzed by infrared spectroscopy. The spectra indicate incorporation of <jats:sup>13<\/jats:sup>C only into the cyanide ligands and not into the CO, showing that cyanide and CO have different metabolic origins. After growth of <jats:italic>E. coli<\/jats:italic> in the presence of <jats:sup>13<\/jats:sup>CO only the CO ligand was labelled with <jats:sup>13<\/jats:sup>C. Labelling did not result from an exchange of the intrinsic CO ligand with the exogenous CO.<\/jats:p>","DOI":"10.1016\/j.febslet.2007.06.028","type":"journal-article","created":{"date-parts":[[2007,6,22]],"date-time":"2007-06-22T11:22:43Z","timestamp":1182511363000},"page":"3317-3321","source":"Crossref","is-referenced-by-count":47,"title":["The CO and CN<sup>\u2212<\/sup> ligands to the active site Fe in [NiFe]\u2010hydrogenase of <i>Escherichia coli<\/i> have different metabolic origins"],"prefix":"10.1002","volume":"581","author":[{"given":"Lucia","family":"Forzi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Hellwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf K.","family":"Thauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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