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The structure is composed of a short antiparallel \u03b2\u2010sheet and four \u03b2\u2010turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, \u03c9\u2010atracotoxin\u2010Hv1a. These observations suggest that agelenin and \u03c9\u2010atracotoxin\u2010Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by \u03c9\u2010agatoxin\u2010IVA and \u03c9\u2010atracotoxin\u2010Hv2a.<\/jats:p>","DOI":"10.1016\/j.febslet.2007.06.077","type":"journal-article","created":{"date-parts":[[2007,7,11]],"date-time":"2007-07-11T07:25:44Z","timestamp":1184138744000},"page":"3789-3794","source":"Crossref","is-referenced-by-count":10,"title":["Solution structure of agelenin, an insecticidal peptide isolated from the spider <i>Agelena opulenta<\/i>, and its structural similarities to insect\u2010specific calcium channel inhibitors"],"prefix":"10.1002","volume":"581","author":[{"given":"Nahoko","family":"Yamaji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Sugase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terumi","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Miki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Wakamori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Iwashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.09.006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00020.2003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(03)00080-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/355827a0"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0406"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.82.5.2284"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0797-559"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102199200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404006200"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105206200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031022"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.11.181"},{"key":"e_1_2_8_14_1","first-page":"351","article-title":"Agelenin&mdash;a new neurotoxin from the venom of the spider, Agelena opulenta","author":"Hagiwara K.","year":"1991","journal-title":"Peptide Chem."},{"key":"e_1_2_8_15_1","first-page":"140","article-title":"Synthesis and disulfide structure determination of agelenin: identification of the carboxy-terminus as an amide form","volume":"5","author":"Inui T.","year":"1992","journal-title":"Peptide Res."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.2004.04322.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)91225-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)90695-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(84)90142-2"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(85)90018-6"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-7807(02)00014-9"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00228148"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360221211"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00009-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00646.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.79.2.622"},{"key":"e_1_2_8_27_1","unstructured":"K. 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