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Potent therapy against HIV, which includes protease inhibitors (PIs), has beneficial effects also on opportunistic infections by pathogens such as <jats:italic>C. neoformans<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic>. PIs inhibit growth of <jats:italic>C. albicans<\/jats:italic> by affecting the activity of its aspartyl proteases. We identified, cloned and sequenced a cDNA from <jats:italic>C. neoformans<\/jats:italic> encoding for a putative aspartyl protease (CnAP1), and the corresponding genomic region. The gene <jats:italic>cnap1<\/jats:italic> codifies for a protein of 505 aa, with a canonical aspartyl protease structure. We purified the recombinant protein and analyzed its activity in the presence of PIs (Indinavir, Lopinavir, Ritonavir), but did not evidence any inhibition of protease activity. The transcriptional level of <jats:italic>cnap1<\/jats:italic> in <jats:italic>C. neoformans<\/jats:italic> is constant in different media. 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