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Recently, the hyper\u2010variable region (HVR) of the cysteine\u2010rich domain was suggested to constitute a potential protein\u2013protein adhesive interface. Here we show that recombinant proteins of HF3, a hemorrhagic P\u2010III SVMP, containing the cysteine\u2010rich domain (disintegrin\u2010like\/cysteine\u2010rich and cysteine\u2010rich proteins) but not the disintegrin\u2010like protein were able to significantly increase leukocyte rolling in the microcirculation. Peptides from the HVR also promoted leukocyte rolling and this activity was inhibited by anti\u2010alpha<jats:sub>M<\/jats:sub>\/beta<jats:sub>2<\/jats:sub> antibodies. These results show, for the first time, that the cysteine\u2010rich domain and its HVR play a role in triggering pro\u2010inflammatory effects mediated by integrins.<\/jats:p>","DOI":"10.1016\/j.febslet.2008.10.034","type":"journal-article","created":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T08:22:26Z","timestamp":1225527746000},"page":"3915-3921","source":"Crossref","is-referenced-by-count":36,"title":["Activation of leukocyte rolling by the cysteine\u2010rich domain and the hyper\u2010variable region of HF3, a snake venom hemorrhagic metalloproteinase"],"prefix":"10.1002","volume":"582","author":[{"given":"Milene C.","family":"Menezes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana F.","family":"Paes Leme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robson L.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maisa","family":"Della Casa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda M.","family":"Bruni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f4nica","family":"Lopes-Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio C.M.","family":"Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay W.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solange M.T.","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-79301999000100002"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01163-9"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.012"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2005.06.007"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06466.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.13094"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00799-3"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(99)01926-5"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1039703"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.07.047"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050483"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604855200"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05895.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601131"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.04.057"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324475"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014465611487"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00131-3"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.02.001"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(86)90094-2"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.012"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020449"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90020-5"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.1.H161"},{"key":"e_1_2_5_26_1","unstructured":"E. 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