{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:12:49Z","timestamp":1790687569873,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T00:00:00Z","timestamp":1267142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIGMS","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH","award":["GM-35332"],"award-info":[{"award-number":["GM-35332"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2010,4,16]]},"abstract":"<jats:p>The in vitro reconstitution of molybdenum nitrogenase was manipulated to generate a chimeric enzyme in which the active site iron\u2013molybdenum cofactor (FeMo\u2010co) is replaced by NifB\u2010co. The NifDK\/NifB\u2010co enzyme was unable to reduce N<jats:sub>2<\/jats:sub> to NH<jats:sub>3<\/jats:sub>, while exhibiting residual C<jats:sub>2<\/jats:sub>H<jats:sub>4<\/jats:sub> and considerable H<jats:sub>2<\/jats:sub> production activities. Production of H<jats:sub>2<\/jats:sub> by NifDK\/NifB\u2010co was stimulated by N<jats:sub>2<\/jats:sub> and was dependent on NifH and ATP hydrolysis. Thus, NifDK\/NifB\u2010co is a useful tool to gain insights into the catalytic mechanism of nitrogenase. Furthermore, phylogenetic analysis of D and K homologs indicates that several early emerging lineages, which contain NifB, NifH and NifDK encoding genes but which lack other genes required for processing NifB\u2010co into FeMo\u2010co, might encode an enzyme with similar catalytic properties to NifDK\/NifB\u2010co.<\/jats:p>","DOI":"10.1016\/j.febslet.2010.02.064","type":"journal-article","created":{"date-parts":[[2010,2,27]],"date-time":"2010-02-27T09:30:59Z","timestamp":1267263059000},"page":"1487-1492","source":"Crossref","is-referenced-by-count":36,"title":["Substrate specificity and evolutionary implications of a NifDK enzyme carrying NifB\u2010co at its active site"],"prefix":"10.1002","volume":"584","author":[{"given":"Basem","family":"Soboh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Boyd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"Rubio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,2,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1529353"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8484118"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073877"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic7018814"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.8.3249"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.070907.103812"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.56.3.979"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2699"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr020608l"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162737"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00466a014"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981165b"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42235-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601115103"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0755358"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708097200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.2.1017-1027.1989"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703050104"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409201102"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002390010061"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh047"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907872106"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.002003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti263"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0908-8857.2004.03297.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.6.1636"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(73)90270-3"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1988.tb02748.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99273-2"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.4.1547-1553.1987"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400965200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00445a040"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr950057h"},{"key":"e_1_2_6_37_1","first-page":"221","volume-title":"Molybdenum Enzymes","author":"Thorneley R.N.F.","year":"1985"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.24.9446"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5395.1853"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/373580a0"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158978"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08299"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2010.02.064","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2010.02.064","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2010.02.064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T04:02:13Z","timestamp":1698638533000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2010.02.064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,26]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,4,16]]}},"alternative-id":["10.1016\/j.febslet.2010.02.064"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2010.02.064","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,26]]}}}