{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:52:21Z","timestamp":1786989141705,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2010,6,18]],"date-time":"2010-06-18T00:00:00Z","timestamp":1276819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007545","name":"AFSSET","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007545","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Leg Poix"},{"name":"CAMPLP"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2010,8,4]]},"abstract":"<jats:p>\n                    Human arylamine\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyltransferase 1 (NAT1) is a xenobiotic\u2010metabolizing enzyme that biotransforms aromatic amine chemicals. We show here that biologically\u2010relevant concentrations of inorganic (Hg\n                    <jats:sup>2+<\/jats:sup>\n                    ) and organic (CH\n                    <jats:sub>3<\/jats:sub>\n                    Hg\n                    <jats:sup>+<\/jats:sup>\n                    ) mercury inhibit the biotransformation functions of NAT1. Both compounds react irreversibly with the active\u2010site cysteine of NAT1 (half\u2010maximal inhibitory concentration (IC\n                    <jats:sub>50<\/jats:sub>\n                    ) = 250 nM and\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>inact<\/jats:sub>\n                    = 1.4 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    M\n                    <jats:sup>\u22121<\/jats:sup>\n                    s\n                    <jats:sup>\u22121<\/jats:sup>\n                    for Hg\n                    <jats:sup>2+<\/jats:sup>\n                    and IC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.4 \u03bcM and\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>inact<\/jats:sub>\n                    = 2 \u00d7 10\n                    <jats:sup>2<\/jats:sup>\n                    M\n                    <jats:sup>\u22121<\/jats:sup>\n                    s\n                    <jats:sup>\u22121<\/jats:sup>\n                    for CH\n                    <jats:sub>3<\/jats:sub>\n                    Hg\n                    <jats:sup>+<\/jats:sup>\n                    ). Exposure of lung epithelial cells led to the inhibition of cellular NAT1 (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 3 and 20 \u03bcM for Hg\n                    <jats:sup>2+<\/jats:sup>\n                    and CH\n                    <jats:sub>3<\/jats:sub>\n                    Hg\n                    <jats:sup>+<\/jats:sup>\n                    , respectively). Our data suggest that exposure to mercury may affect the biotransformation of aromatic amines by NAT1.\n                  <\/jats:p>","DOI":"10.1016\/j.febslet.2010.06.022","type":"journal-article","created":{"date-parts":[[2010,6,20]],"date-time":"2010-06-20T20:25:03Z","timestamp":1277065503000},"page":"3366-3369","source":"Crossref","is-referenced-by-count":11,"title":["The human xenobiotic\u2010metabolizing enzyme arylamine\n                    <i>N<\/i>\n                    \u2010acetyltransferase 1 (NAT1) is irreversibly inhibited by inorganic (Hg\n                    <sup>2+<\/sup>\n                    ) and organic mercury (CH\n                    <sub>3<\/sub>\n                    Hg\n                    <sup>+<\/sup>\n                    ): Mechanism and kinetics"],"prefix":"10.1002","volume":"584","author":[{"given":"Nilusha","family":"Ragunathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Busi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Pluvinage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Sanfins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Dupret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Rodrigues-Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Dairou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,6,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/S0090-9556(24)13670-7","article-title":"Pharmacogenetics of the arylamine N-acetyltransferases: a symposium in honor of Wendell W. 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