{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:08:43Z","timestamp":1791187723970,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2010,9,15]],"date-time":"2010-09-15T00:00:00Z","timestamp":1284508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2010,10,8]]},"abstract":"<jats:p>\n                    HydG uses tyrosine to synthesize the CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/CO ligands of [FeFe]\u2010hydrogenase active site. We have mutated two of the [4Fe\u20134S]\u2010cluster cysteine ligands of the HydG C\u2010terminal domain (CTD) to serine. The double mutant can still synthesize CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    but not CO. In a mutant lacking the CTD both CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    and CO synthesis are abolished. Like in ThiH, the initial steps of CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    synthesis are carried out in the TIM\u2010barrel domain of HydG but some component(s) of the CTD are later needed. The mutants indicate that CO synthesis is metal\u2010based and occurs in the CTD. We postulate that CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/CO synthesis is initiated by H\n                    <jats:sub>2<\/jats:sub>\n                    N\u2013CH\u2013\n                    <jats:disp-formula>\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" position=\"anchor\" xlink:href=\"graphic\/feb2s0014579310007283-math-si1.gif\">\n                        <jats:alt-text>urn:x-wiley:00145793:media:feb2s0014579310007283:feb2s0014579310007283-math-si1<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:disp-formula>\n                    Fragmentation of this radical into H\n                    <jats:sub>2<\/jats:sub>\n                    N\u2013CH\n                    <jats:sub>2<\/jats:sub>\n                    and CO\n                    <jats:sub>2<\/jats:sub>\n                    or H\n                    <jats:sub>2<\/jats:sub>\n                    CNH and\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/feb2s0014579310007283-math-si2.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2s0014579310007283:feb2s0014579310007283-math-si2\"\/>\n                    provides plausible precursors for CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/CO synthesis.\n                  <\/jats:p>","DOI":"10.1016\/j.febslet.2010.09.008","type":"journal-article","created":{"date-parts":[[2010,9,17]],"date-time":"2010-09-17T04:19:42Z","timestamp":1284697182000},"page":"4197-4202","source":"Crossref","is-referenced-by-count":56,"title":["A glycyl free radical as the precursor in the synthesis of carbon monoxide and cyanide by the [FeFe]\u2010hydrogenase maturase HydG"],"prefix":"10.1002","volume":"584","author":[{"given":"Yvain","family":"Nicolet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Tron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Fontecilla-Camps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050195z"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.01.017"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(06)51001-X"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08299"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(99)80005-7"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048837k"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403206200"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.07.092"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510310200"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh728"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.01.004"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007565"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-007-0224-z"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.04.063"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001937107"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.12.016"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200907047"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja1012273"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801161200"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200702554"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.6.2163-2172.2006"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)58067-9"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(78)90285-0"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3331"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.056606"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00439a069"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja9815428"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00800a038"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00261a031"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp004567d"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi034902z"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/b508403b"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.12.040"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03288.x"}],"container-title":["FEBS 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