{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:33:41Z","timestamp":1789882421833,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,3,12]],"date-time":"2011-03-12T00:00:00Z","timestamp":1299888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2011,3,12]],"date-time":"2011-03-12T00:00:00Z","timestamp":1299888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,6]]},"DOI":"10.1016\/j.febslet.2011.03.012","type":"journal-article","created":{"date-parts":[[2011,3,13]],"date-time":"2011-03-13T04:11:30Z","timestamp":1299989490000},"page":"1061-1066","source":"Crossref","is-referenced-by-count":104,"title":["Structural basis for a new tetracycline resistance mechanism relying on the TetX monooxygenase"],"prefix":"10.1002","volume":"585","author":[{"given":"Gesa","family":"Volkers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gottfried J.","family":"Palm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred S.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard D.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried","family":"Hinrichs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,12]]},"reference":[{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0005|feb2s0014579311001657-cit-b0005","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1179\/joc.2008.20.4.411","article-title":"Tigecycline: a critical update","volume":"20","author":"Shakil","year":"2008","journal-title":"J. Chemother."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0010|feb2s0014579311001657-cit-b0010","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1128\/AAC.01499-05","article-title":"Functional, biophysical, and structural bases for antibacterial activity of tigecycline","volume":"50","author":"Olson","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0015|feb2s0014579311001657-cit-b0015","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S1473-3099(10)70143-2","article-title":"Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study","volume":"10","author":"Kumarasamy","year":"2010","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0020|feb2s0014579311001657-cit-b0020","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1128\/AAC.01198-06","article-title":"Tigecycline efflux as a mechanism for nonsusceptibility in Acinetobacter baumannii","volume":"51","author":"Peleg","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0025|feb2s0014579311001657-cit-b0025","doi-asserted-by":"crossref","first-page":"52346","DOI":"10.1074\/jbc.M409573200","article-title":"TetX is a flavin-dependent monooxygenase conferring resistance to tetracycline antibiotics","volume":"279","author":"Yang","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0030|feb2s0014579311001657-cit-b0030","doi-asserted-by":"crossref","first-page":"11829","DOI":"10.1021\/bi0506066","article-title":"Tigecycline is modified by the flavin-dependent monooxygenase TetX","volume":"44","author":"Moore","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0035|feb2s0014579311001657-cit-b0035","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/S0092-8674(00)00216-6","article-title":"The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit","volume":"103","author":"Brodersen","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0040|feb2s0014579311001657-cit-b0040","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1093\/emboj\/20.8.1829","article-title":"Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3","volume":"20","author":"Pioletti","year":"2001","journal-title":"EMBO J."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0045|feb2s0014579311001657-cit-b0045","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1128\/AAC.32.12.1797","article-title":"The cryptic tetracycline resistance determinant on Tn4400 mediates tetracycline degradation as well as tetracycline efflux","volume":"32","author":"Park","year":"1988","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0050|feb2s0014579311001657-cit-b0050","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/JB.173.1.176-183.1991","article-title":"Evidence that a novel tetracycline resistance gene found on two Bacteroides transposons encodes an NADP-requiring oxidoreductase","volume":"173","author":"Speer","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0055|feb2s0014579311001657-cit-b0055","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1128\/JB.170.4.1423-1429.1988","article-title":"Characterization of a novel tetracycline resistance that functions only in aerobically grown Escherichia coli","volume":"170","author":"Speer","year":"1988","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0060|feb2s0014579311001657-cit-b0060","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1111\/j.1365-2672.2008.04101.x","article-title":"Sphingobacterium sp. strain PM2-P1-29 harbours a functional tet(X) gene encoding for the degradation of tetracycline","volume":"106","author":"Ghosh","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0065|feb2s0014579311001657-cit-b0065","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.mib.2007.08.009","article-title":"Expanding the soil antibiotic resistome: exploring environmental diversity","volume":"10","author":"D&rsquo;Costa","year":"2007","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0070|feb2s0014579311001657-cit-b0070","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1002\/j.1460-2075.1990.tb08287.x","article-title":"Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure","volume":"9","author":"Hendrickson","year":"1990","journal-title":"EMBO J."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0075|feb2s0014579311001657-cit-b0075","first-page":"611","article-title":"Crystallization and preliminary X-ray crystallographic analysis of the tetracycline-degrading monooxygenase TetX2 from Bacteroides thetaiotaomicron","volume":"F66","author":"Volkers","year":"2010","journal-title":"Acta Crystallogr."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0080|feb2s0014579311001657-cit-b0080","first-page":"760","article-title":"The CCP4 suite: programs for protein crystallography","volume":"D50","author":"CCP4","year":"1994","journal-title":"Acta Crystallogr."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0085|feb2s0014579311001657-cit-b0085","first-page":"240","article-title":"Refinement of macromolecular structures by the maximum-likelihood method","volume":"D53","author":"Murshudov","year":"1997","journal-title":"Acta Crystallogr."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0090|feb2s0014579311001657-cit-b0090","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","article-title":"Inference of macromolecular assemblies from crystalline state","volume":"372","author":"Krissinel","year":"2007","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0095|feb2s0014579311001657-cit-b0095","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0022-2836(73)90388-4","article-title":"Comparison of super-secondary structures in proteins","volume":"76","author":"Rao","year":"1973","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0100|feb2s0014579311001657-cit-b0100","doi-asserted-by":"crossref","first-page":"W545","DOI":"10.1093\/nar\/gkq366","article-title":"Dali server: conservation mapping in 3D","volume":"38","author":"Holm","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0105|feb2s0014579311001657-cit-b0105","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0301-4622(97)00126-9","article-title":"The PHBH fold: not only flavoenzymes","volume":"70","author":"Mattevi","year":"1998","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0110|feb2s0014579311001657-cit-b0110","doi-asserted-by":"crossref","first-page":"22459","DOI":"10.1016\/S0021-9258(17)31664-2","article-title":"Activation of molecular oxygen by flavins and flavoproteins","volume":"269","author":"Massey","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0115|feb2s0014579311001657-cit-b0115","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1021\/bi00327a012","article-title":"Interaction of pyrophosphate moieties with a-helixes in dinucleotide binding proteins","volume":"24","author":"Wierenga","year":"1985","journal-title":"Biochemistry"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0120|feb2s0014579311001657-cit-b0120","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1128\/AEM.62.7.2405-2410.1996","article-title":"Analysis of interaction between the Arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis","volume":"62","author":"Nishiya","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0125|feb2s0014579311001657-cit-b0125","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.jmb.2007.06.087","article-title":"Crystal structures of two aromatic hydroxylases involved in the early tailoring steps of angucycline biosynthesis","volume":"372","author":"Koskiniemi","year":"2007","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0130|feb2s0014579311001657-cit-b0130","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1126\/science.7939628","article-title":"The mobile flavin of 4-OH benzoate hydroxylase","volume":"266","author":"Gatti","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0135|feb2s0014579311001657-cit-b0135","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1002\/pro.5560031210","article-title":"Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified FAD present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin","volume":"3","author":"van Berkel","year":"1994","journal-title":"Protein Sci."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0140|feb2s0014579311001657-cit-b0140","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1073\/pnas.022640199","article-title":"Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase","volume":"99","author":"Wang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0145|feb2s0014579311001657-cit-b0145","doi-asserted-by":"crossref","first-page":"13506","DOI":"10.1021\/bi801229w","article-title":"The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction","volume":"47","author":"Ryan","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0150|feb2s0014579311001657-cit-b0150","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/0022-2836(89)90158-7","article-title":"Crystal structure of the p-hydroxybenzoate hydroxylase&ndash;substrate complex refined at 1.9 &Aring; resolution. Analysis of the enzyme&ndash;substrate and enzyme&ndash;product complexes","volume":"208","author":"Schreuder","year":"1989","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0155|feb2s0014579311001657-cit-b0155","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.jmb.2009.09.003","article-title":"Structural basis for substrate recognition and specificity in aklavinone-11-hydroxylase from rhodomycin biosynthesis","volume":"393","author":"Lindqvist","year":"2009","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0160|feb2s0014579311001657-cit-b0160","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.jmb.2006.09.031","article-title":"Crystal structure of 3-hydroxybenzoate hydroxylase from Comamonas testosteroni has a large tunnel for substrate and oxygen access to the active site","volume":"364","author":"Hiromoto","year":"2006","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0165|feb2s0014579311001657-cit-b0165","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1126\/science.8153629","article-title":"Structure of the Tet repressor&ndash;tetracycline complex and regulation of antibiotic resistance","volume":"264","author":"Hinrichs","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.febslet.2011.03.012-BIB2s0014579311001657-bib-b0170|feb2s0014579311001657-cit-b0170","doi-asserted-by":"crossref","first-page":"17913","DOI":"10.1021\/ja806629e","article-title":"A robust platform for the synthesis of new tetracycline antibiotics","volume":"130","author":"Sun","year":"2008","journal-title":"J. Am. Chem. Soc."}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2011.03.012","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2011.03.012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1016\/j.febslet.2011.03.012\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T03:03:08Z","timestamp":1637377388000},"score":1,"resource":{"primary":{"URL":"http:\/\/doi.wiley.com\/10.1016\/j.febslet.2011.03.012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,12]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,4,6]]}},"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2011.03.012","archive":["Portico"],"relation":{},"ISSN":["0014-5793"],"issn-type":[{"value":"0014-5793","type":"print"}],"subject":[],"published":{"date-parts":[[2011,3,12]]}}}