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Autoglucosylation, maltoside transglucosylation and UDP\u2010glucose hydrolyzing activities were abolished even though affinity and interactions with UDP\u2010glucose and positioning of Tyr194 acceptor were conserved. Substitution of Thr82 for serine but not for valine restored the maximum extent of autoglucosylation as well as transglucosylation and UDP\u2010glucose hydrolysis rate. 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