{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T07:54:58Z","timestamp":1778831698893,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T00:00:00Z","timestamp":1340064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2012,7,30]]},"abstract":"<jats:p>\n<jats:sc>d<\/jats:sc>\u2010Galacturonic acid is a major component of pectins but cannot be metabolized by <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. It is assumed not to be taken up. We show that yeast displays surprisingly rapid low\u2010affinity uptake of <jats:sc>d<\/jats:sc>\u2010galacturonic acid, strongly increasing with decreasing extracellular pH and without saturation up to 1.5 M. There was no intracellular concentration above the extracellular level and transport was reversible. Among more than 160 single and multiple deletion mutants in channels and transporters, no strain was affected in <jats:sc>d<\/jats:sc>\u2010galacturonic acid uptake. The uptake was not inhibited by any compound tested as candidate competitive inhibitor, including <jats:sc>d<\/jats:sc>\u2010glucuronic acid, which was also transported. The characteristics of <jats:sc>d<\/jats:sc>\u2010galacturonic acid uptake are consistent with involvement of a channel\u2010type system, probably encoded by multiple genes.<\/jats:p>","DOI":"10.1016\/j.febslet.2012.06.012","type":"journal-article","created":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T13:07:39Z","timestamp":1340111259000},"page":"2494-2499","source":"Crossref","is-referenced-by-count":23,"title":["Evidence for rapid uptake of <scp>d<\/scp>\u2010galacturonic acid in the yeast <i>Saccharomyces cerevisiae<\/i> by a channel\u2010type transport system"],"prefix":"10.1002","volume":"586","author":[{"given":"Ben","family":"Souffriau","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"den Abt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan M.","family":"Thevelein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bk-1994-0566.ch019"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-1870-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(08)70597-5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02565.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.13.4263-4265.1993"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1536(77)80083-1"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2008.08.002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-006-9085-7"},{"key":"e_1_2_8_10_1","unstructured":"Huisjes E.H. de Hulster E. van Dam J.C. 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