{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:51:44Z","timestamp":1791273104717,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T00:00:00Z","timestamp":1348531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2012,11,2]]},"abstract":"<jats:p>The HypC and HypD maturases are required for the biosynthesis of the Fe(CN)<jats:sub>2<\/jats:sub>CO cofactor in the large subunit of [NiFe]\u2010hydrogenases. Using infrared spectroscopy we demonstrate that an anaerobically purified, Strep\u2010tagged HypCD complex from <jats:italic>Escherichia coli<\/jats:italic> exhibits absorption bands characteristic of diatomic CO and CN<jats:sup>\u2212<\/jats:sup> ligands as well as CO<jats:sub>2<\/jats:sub>. Metal and sulphide analyses revealed that along with the [4Fe\u20134S]<jats:sup>2+<\/jats:sup> cluster in HypD, the complex has two additional oxygen\u2010labile Fe ions. We prove that HypD cysteine 41 is required for the coordination of all three ligands. These findings suggest that the HypCD complex carries minimally the Fe(CN)<jats:sub>2<\/jats:sub>CO cofactor.<\/jats:p>","DOI":"10.1016\/j.febslet.2012.09.019","type":"journal-article","created":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T18:58:19Z","timestamp":1348599499000},"page":"3882-3887","source":"Crossref","is-referenced-by-count":39,"title":["[NiFe]\u2010hydrogenase maturation: Isolation of a HypC\u2013HypD complex carrying diatomic CO and CN<sup>\u2212<\/sup> ligands"],"prefix":"10.1002","volume":"586","author":[{"given":"Basem","family":"Soboh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven T.","family":"Stripp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Muhr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Granich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Braussemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Herzberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Heberle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Gary Sawers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,25]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"455","article-title":"Classification and phylogeny of hydrogenases, FEMS","volume":"25","author":"Vignais P.M.","year":"2001","journal-title":"Microbiol. Rev."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.030508.152103"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/373580a0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3331"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(06)51001-X"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-006-9048-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080972"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204601200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00276307"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.12.013"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.06.028"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.06.027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.09.023"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.387134"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.09.040"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.05.039"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.06.045"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9720078"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808488200"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01070-7"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(76)90119-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90385-2"},{"key":"e_1_2_6_24_1","unstructured":"J. Sambrook D.W. Russell 2001 Cold Spring Harbor Laboratory Press Plainview NY"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1977.tb00905.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0726-5"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.06.096"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)58067-9"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1969.14.3.0454"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0629p.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0924-2031(92)87018-B"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.12.016"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.08.037"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2012.09.019","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2012.09.019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2012.09.019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:45:30Z","timestamp":1698666330000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2012.09.019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,25]]},"references-count":34,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2012,11,2]]}},"alternative-id":["10.1016\/j.febslet.2012.09.019"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2012.09.019","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,25]]}}}