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We aimed to identify a homologous sensor in <jats:italic>Brucella abortus<\/jats:italic>. A predicted protein with transmembrane and conserved histidine kinase domains was identified as the Fix\u2010like <jats:italic>Brucella<\/jats:italic> sensor, FlbS. Although it lacks the PAS domain, recombinant FlbS binds haem in vitro. An internal in\u2010frame deletion in <jats:italic>flbS<\/jats:italic> severely decreased <jats:italic>B. abortus<\/jats:italic> survival inside professional and non\u2010professional phagocytes. This phenotype was reverted by genetic complementation. These results indicate the critical role of this haemoprotein in the intracellular lifestyle of <jats:italic>Brucella<\/jats:italic>.<\/jats:p>","DOI":"10.1016\/j.febslet.2013.07.047","type":"journal-article","created":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T04:03:40Z","timestamp":1376453020000},"page":"3102-3107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["FixL\u2010like sensor FlbS of <i>Brucella abortus<\/i> binds haem and is necessary for survival within eukaryotic cells"],"prefix":"10.1002","volume":"587","author":[{"given":"Mara S.","family":"Roset","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta A.","family":"Almir\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800266"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.8.2454-2462.2001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01185-06"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1615-1619.2006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1973-1976.2006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08095.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1297-1303.2000"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/152165401753544232"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2005.02.016"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)00240-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2008.09.018"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07005.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04316.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7347"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6225-6230.2001"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01514-07"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.9.3013-3020.1997"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28213-0"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.4.1103-1109.1993"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503022102"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.9.3469"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6557-6567.1990"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2013.07.047","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2013.07.047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T04:33:50Z","timestamp":1698640430000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2013.07.047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,13]]},"references-count":25,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2013,9,17]]}},"alternative-id":["10.1016\/j.febslet.2013.07.047"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2013.07.047","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,13]]},"assertion":[{"value":"2013-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}