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Molecular modelling of the enzyme predicted that a Ser\u2010Ala\u2010Asp motif which is believed to be a helminth\u2010specific epitope is exposed. The enzyme is dimeric (as judged by gel filtration and cross\u2010linking), resistant to proteolysis and highly stable to thermal denaturation (melting temperature of 82.0 \u00b0C). The steady\u2010state kinetic parameters are high (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    for dihydroxyacetone phosphate is 0.51 mM;\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    for glyceraldehyde 3\u2010phosphate is 1.1 mM;\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>cat<\/jats:sub>\n                    for dihydroxyacetone phosphate is 7800 s\n                    <jats:sup>\u22121<\/jats:sup>\n                    and\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>cat<\/jats:sub>\n                    for glyceraldehyde 3\u2010phosphate is 6.9 s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1016\/j.febslet.2013.09.022","type":"journal-article","created":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T20:37:08Z","timestamp":1379968628000},"page":"3422-3427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Triose phosphate isomerase from the blood fluke\n                    <i>Schistosoma mansoni<\/i>\n                    : Biochemical characterisation of a potential drug and vaccine target"],"prefix":"10.1002","volume":"587","author":[{"given":"Veronika L.","family":"Zinsser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Farnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Dunne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Timson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69440-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328357e4cf"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3151-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.83"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2307\/4134531"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2009.09.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540601115960"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.20.7903"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.087551"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000030"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050993"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2012.12.020"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.05.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.5.1842"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.148.2.562"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(91)90191-P"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2249-4"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.05.009"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012000339"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500368"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2011.10.021"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2011.00392.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.028068-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2007.00292.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.05.017"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00231-09"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.04.080"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182005008644"},{"key":"e_1_2_8_30_1","first-page":"518","article-title":"The protective immunity of a DNA vaccine encoding Schistosoma japonicum Chinese strain triose-phosphate isomerase in infected BALB\/C mice","volume":"35","author":"Zhu Y.","year":"2004","journal-title":"Southeast Asian J. 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