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Only scoulerine 9\u2010<jats:italic>O<\/jats:italic>\u2010methyltransferase has been functionally characterized. We report the isolation and characterization of a cytochrome P450 (CYP719A21) from opium poppy that converts (<jats:italic>S<\/jats:italic>)\u2010tetrahydrocolumbamine to (<jats:italic>S<\/jats:italic>)\u2010canadine. Recombinant CYP719A21 displayed strict substrate specificity and high affinity (<jats:italic>K<jats:sub>m<\/jats:sub>\n<\/jats:italic>\n= 4.63 \u00b1 0.71 \u03bcM) for (<jats:italic>S<\/jats:italic>)\u2010tetrahydrocolumbamine. Virus\u2010induced gene silencing of <jats:italic>CYP719A21<\/jats:italic> caused a significant increase in (<jats:italic>S<\/jats:italic>)\u2010tetrahydrocolumbamine accumulation and a corresponding decrease in the levels of putative downstream intermediates and noscapine in opium poppy plants.<\/jats:p>","DOI":"10.1016\/j.febslet.2013.11.037","type":"journal-article","created":{"date-parts":[[2013,12,5]],"date-time":"2013-12-05T06:45:58Z","timestamp":1386225958000},"page":"198-204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Cloning and characterization of canadine synthase involved in noscapine biosynthesis in opium poppy"],"prefix":"10.1002","volume":"588","author":[{"given":"Thu-Thuy T.","family":"Dang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter 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