{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:55:15Z","timestamp":1790517315679,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,1,25]],"date-time":"2014-01-25T00:00:00Z","timestamp":1390608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2014,3,3]]},"abstract":"<jats:p>Although AHCYL2 (long\u2010IRBIT) is highly homologous to IRBIT, which regulates ion\u2010transporting proteins including the electrogenic Na<jats:sup>+<\/jats:sup>\u2010HCO<jats:sub>3<\/jats:sub>\n<jats:sup>\u2212<\/jats:sup> cotransporter NBCe1\u2010B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1\u2010B in bovine parotid acinar cells using yeast two\u2010hybrid, immunofluorescence confocal microscopy and co\u2010immunoprecipitation analyses. Whole\u2010cell patch\u2010clamp experiments revealed that co\u2010expression of AHCYL2 reduces the apparent affinity for intracellular Mg<jats:sup>2+<\/jats:sup> in inhibition of NBCe1\u2010B currents specifically in a HCO<jats:sub>3<\/jats:sub>\n<jats:sup>\u2212<\/jats:sup>\u2010deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1\u2010B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT.<\/jats:p>","DOI":"10.1016\/j.febslet.2013.12.036","type":"journal-article","created":{"date-parts":[[2014,1,25]],"date-time":"2014-01-25T21:30:32Z","timestamp":1390685432000},"page":"672-677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["AHCYL2 (long\u2010IRBIT) as a potential regulator of the electrogenic Na<sup>+<\/sup>\u2010HCO<sub>3<\/sub><sup>\u2212<\/sup> cotransporter NBCe1\u2010B"],"prefix":"10.1002","volume":"588","author":[{"given":"Soichiro","family":"Yamaguchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.10.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602250103"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210119200"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43475"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00352.2011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.05.017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070361"},{"key":"e_1_2_7_9_1","first-page":"193","article-title":"IRBIT coordinates epithelial fluid and HCO3\n                  \u2212 secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct","volume":"119","author":"Yang D.","year":"2009","journal-title":"J. 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