{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T02:42:45Z","timestamp":1784774565329,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2014,5,21]],"date-time":"2014-05-21T00:00:00Z","timestamp":1400630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2014,6,27]]},"abstract":"<jats:p>Enolases are highly conserved metalloenzymes ubiquitous to cellular metabolism. While these enzymes share a large degree of sequence and structural similarity, they have been shown to possess a wide range of moonlighting functions. Recent studies showed that an enolase from <jats:italic>Lactobacillus gasseri<\/jats:italic> impedes the ability of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> to adhere to epithelial cells. We present the crystal structure of this enolase, the first from <jats:italic>Lactobacillus<\/jats:italic>, with one of its Mg<jats:sup>2+<\/jats:sup> cofactors. Determined using molecular replacement to 2.08 \u00c5, the structure has a flexible and surface exposed catalytic loop containing lysines, and may play a role in the inhibitory function.<\/jats:p>","DOI":"10.1016\/j.febslet.2014.05.020","type":"journal-article","created":{"date-parts":[[2014,5,21]],"date-time":"2014-05-21T09:01:59Z","timestamp":1400662919000},"page":"2212-2216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Crystal structure of an efficacious gonococcal adherence inhibitor: An enolase from <i>Lactobacillus gasseri<\/i>"],"prefix":"10.1002","volume":"588","author":[{"given":"Kannan","family":"Raghunathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel R.","family":"Spurbeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy G.","family":"Arvidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis N.","family":"Arvidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.01850.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.5.709"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01200-09"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000910"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.385706"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-8-14"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.10.098"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01966-06"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03557.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1160\/TH05-05-0369","article-title":"The nine residue plasminogen-binding motif of the pneumococcal enolase is the major cofactor of plasmin-mediated degradation of extracellular matrix, dissolution of fibrin and transmigration","volume":"94","author":"Bergmann S.","year":"2005","journal-title":"Thromb. Haemost."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.06.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00085"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.028795-0"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013826"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-012-2040-7"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00197a038"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9712450"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.050"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.08.088"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088395"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028481"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/017145-0"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.003830-0"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2012.10.001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309110022748"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309110012078"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910048675"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10969-012-9129-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889812017293"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444907047580"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1107\/S090744490705024X"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444912001308"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh477"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq313"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.022"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr484"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn860"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvg104"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00013a022"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.06027.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.03.017"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02448.x"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018119"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027502"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/000901-0"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28747-0"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00893-3"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00225a013"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340160302"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2014.05.020","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2014.05.020","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T23:40:29Z","timestamp":1698450029000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2014.05.020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,21]]},"references-count":56,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2014,6,27]]}},"alternative-id":["10.1016\/j.febslet.2014.05.020"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2014.05.020","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,21]]},"assertion":[{"value":"2014-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}