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Two distinct <jats:italic>rspo2<\/jats:italic> zebrafish mutants were generated by TALEN\u2010mediated mutagenesis: a null mutant, <jats:italic>rspo2<jats:sup>null<\/jats:sup>\n<\/jats:italic>, lacking all functional domains, and a hypomorphic mutant, <jats:italic>rspo2<jats:sup>tsp<\/jats:sup>\n<\/jats:italic>, lacking the two N\u2010terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the <jats:italic>rspo2<jats:sup>tsp<\/jats:sup>\n<\/jats:italic> mutants, but were absent from the <jats:italic>rspo2<jats:sup>null<\/jats:sup>\n<\/jats:italic> mutants. Hypoplasia of the neural\/hemal arches and ribs was observed in both mutants. Thus, the two <jats:italic>rspo2<\/jats:italic> mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.<\/jats:p>","DOI":"10.1016\/j.febslet.2014.10.015","type":"journal-article","created":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T16:40:34Z","timestamp":1414860034000},"page":"4543-4550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["TALEN\u2010mediated mutagenesis in zebrafish reveals a role for <i>r\u2010spondin 2<\/i> in fin ray and vertebral development"],"prefix":"10.1002","volume":"588","author":[{"given":"Yoshiaki","family":"Tatsumi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moe","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Yokoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.07.019"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106083108"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040976"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.175"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2012.09.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3787"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.01.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-3-242"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.01.006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2007.00978.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.013359"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.08.023"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.02.066"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2006.08.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808337200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30625"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg892"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks518"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002861"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214004110"},{"key":"e_1_2_6_22_1","unstructured":"M. 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