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The structure shows the motor domain with an open active site and the large cleft that divides the 50kDa domain in a closed state. Compared to other rigor\u2010like myosin motor domain structures, our structure shows subtle but significant conformational changes in regions important for actin binding and mechanochemical coupling. Moreover, our crystal structure helps to rationalize the impact of myosin, heavy chain 9 (<jats:italic>MYH9<\/jats:italic>)\u2010related disease mutations Arg709Cys and Arg709His on the kinetic and functional properties of NM\u20102B and of the closely related non\u2010muscle myosin 2A (NM\u20102A).<\/jats:p>","DOI":"10.1016\/j.febslet.2014.11.007","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T16:26:22Z","timestamp":1416241582000},"page":"4754-4760","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Crystal structure of the rigor\u2010like human non\u2010muscle myosin\u20102 motor domain"],"prefix":"10.1002","volume":"588","author":[{"given":"Stefan","family":"M\u00fcnnich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma","family":"Pathan-Chhatbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar J.","family":"Manstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.094300"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100809-151502"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-9-r196"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1002-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37123-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14533"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Leukozyteneinschl\u00fcsse","volume":"96","author":"May R.","year":"1909","journal-title":"Dtsch. 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