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The aim of this study was to develop a novel assay for the screening of anti\u2010aggregation compounds with a focus on the aggregation\u2010promoting capacity of GAPDH. The assay includes a pure Q58 polyglutamine fragment, GAPDH, and a transglutaminase that links the two proteins. The feasibility of the new assay was verified using two GAPDH binders, hydroxynonenal and \u2212(\u2212)deprenyl, and the benzothiazole derivative PGL\u2010135 which exhibits anti\u2010aggregation effect. 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