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We have here used phage display, bacterial two\u2010hybrid and fluorescence\u2010based interaction assays to identify an N\u2010terminal domain of the inner membrane protein VirB6 as a site of interaction with the envelope\u2010spanning VirB10 protein. Our results are consistent with the notion that VirB6 acts in concert with VirB10 as well as with VirB8 during secretion system assembly and function.<\/jats:p>","DOI":"10.1016\/j.febslet.2015.05.051","type":"journal-article","created":{"date-parts":[[2015,6,13]],"date-time":"2015-06-13T00:51:25Z","timestamp":1434156685000},"page":"1883-1889","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["VirB6 and VirB10 from the <i>Brucella<\/i> type IV secretion system interact via the N\u2010terminal periplasmic domain of VirB6"],"prefix":"10.1002","volume":"589","author":[{"given":"Ana Maria","family":"Villamil Giraldo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charline","family":"Mary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durgajini","family":"Sivanesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2218"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2014.02.006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08588"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166101"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13081"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095211"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300298v"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.06.052"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.882-893.2006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405843101"},{"key":"e_1_2_6_12_1","unstructured":"T.A. 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