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Pacodine and palaudine are tri\u2010<jats:italic>O<\/jats:italic>\u2010methylated analogs of papaverine, which contains four<jats:italic>O<\/jats:italic>\u2010linked methyl groups. However, the biosynthetic origin of pacodine and palaudine has not been established. Three members of the 2\u2010oxoglutarate\/Fe<jats:sup>2+<\/jats:sup>\u2010dependent dioxygenases (2ODDs) family in opium poppy display widespread<jats:italic>O<\/jats:italic>\u2010dealkylation activity on several benzylisoquinoline alkaloids with diverse structural scaffolds, and two are responsible for the antepenultimate and ultimate steps in morphine biosynthesis. We report a novel 2ODD from opium poppy catalyzing the efficient substrate\u2010 and regio\u2010specific 7\u2010<jats:italic>O<\/jats:italic>\u2010demethylation of papaverine yielding pacodine. The occurrence of papaverine 7\u2010<jats:italic>O<\/jats:italic>\u2010demethylase (P7ODM) expands the enzymatic scope of the 2ODD family in opium poppy and suggests an unexpected biosynthetic route to pacodine.<\/jats:p>","DOI":"10.1016\/j.febslet.2015.07.042","type":"journal-article","created":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T22:17:33Z","timestamp":1439072253000},"page":"2701-2706","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Papaverine 7\u2010<i>O<\/i>\u2010demethylase, a novel 2\u2010oxoglutarate\/Fe<sup>2+<\/sup>\u2010dependent dioxygenase from opium poppy"],"prefix":"10.1002","volume":"589","author":[{"given":"Scott C.","family":"Farrow","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Facchini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,8]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Bern\u00e1th J. 1998. 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