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The majority of studies, however, have revealed that the effect of \u03b1\u2010synuclein oligomers is only noticeable on systems composed of anionic lipids, while the more physiologically relevant zwitterionic lipids remain intact. We present experimental evidence for significant morphological changes in zwitterionic membranes containing cholesterol, induced by \u03b1\u2010synuclein oligomers. Depending on the lipid composition, model membranes are either unperturbed, disrupt, or undergo dramatic morphological changes and segregate into structurally different components, which we visualize by 2\u2010photon fluorescence microscopy and generalized polarization analysis using the fluorescent probe Laurdan. 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