{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T10:26:18Z","timestamp":1773829578378,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2015,9,28]],"date-time":"2015-09-28T00:00:00Z","timestamp":1443398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"German Research Foundation (DFG)","award":["SFB960"],"award-info":[{"award-number":["SFB960"]}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2015,10,24]]},"abstract":"<jats:p>The<jats:italic>Arabidopsis<\/jats:italic> multidomain protein SPT5L\/KTF1 (which has similarity to the transcript elongation factor SPT5) associates with RNA polymerase V (RNAPV) and is an accessory factor in RNA\u2010directed DNA methylation. The zinc\u2010finger protein SPT4 was found to interact with SPT5L (and SPT5) both in vivo and in vitro. Here, we show that plants depleted of SPT4 relative to wild type display reduced DNA methylation and the locus specificity is shared with SPT5L, suggesting a cooperation of SPT4 and SPT5L. Unlike observed for SPT5, no reduced protein level of SPT5L is determined in SPT4\u2010deficient plants. These experiments demonstrate that in addition to the RNA polymerase II\u2010associated SPT4\/SPT5 that is generally conserved in eukaryotes, flowering plants have SPT4\/SPT5L that is involved in RNAPV\u2010mediated transcriptional silencing.<\/jats:p>","DOI":"10.1016\/j.febslet.2015.09.017","type":"journal-article","created":{"date-parts":[[2015,9,30]],"date-time":"2015-09-30T04:19:41Z","timestamp":1443586781000},"page":"3254-3257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The zinc\u2010finger protein SPT4 interacts with SPT5L\/KTF1 and modulates transcriptional silencing 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