{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:56:14Z","timestamp":1780419374745,"version":"3.54.1"},"reference-count":47,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":4470,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","fertstert.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fertility and Sterility"],"published-print":{"date-parts":[[2010,3]]},"DOI":"10.1016\/j.fertnstert.2009.02.061","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T05:06:22Z","timestamp":1238648782000},"page":"1415-1420","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Changing P2X receptor localization on maturing sperm in the epididymides of mice, hamsters, rats, and humans: a preliminary study"],"prefix":"10.1016","volume":"93","author":[{"given":"Frederick C.L.","family":"Banks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Calvert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Burnstock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fertnstert.2009.02.061_bib1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1095\/biolreprod15.5.586","article-title":"Daily spermatazoal production epididymal spermatozoal reserves and transit time of spermatozoa through the epididymis of the Rhesus monkey","volume":"15","author":"Amann","year":"1976","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib2","first-page":"229","article-title":"Structure and function of the epididymis","volume":"14","author":"Consentino","year":"1986","journal-title":"Urol Res"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib3","first-page":"73","article-title":"Plasma membrane structure and remodelling during sperm maturation in the epididymis","volume":"53","author":"Jones","year":"1998","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib4","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1046\/j.1365-2605.1999.00151.x","article-title":"To store or mature spermatozoa? The primary role of the epididymis","volume":"22","author":"Jones","year":"1999","journal-title":"Int J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib5","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1095\/biolreprod55.5.956","article-title":"Characterization and identification of proteins secreted in the various regions of the adult boar epididymis","volume":"55","author":"Syntin","year":"1996","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/jemt.10312","article-title":"Contribution of epididymal secretory proteins for spermatozoa maturation","volume":"61","author":"Dacheux","year":"2003","journal-title":"Microsc Res Tech"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib7","series-title":"Human anatomy and physiology","year":"1992"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1463-6395.1959.tb00389.x","article-title":"Epididymal structure and function. A historic and critical review","volume":"40","author":"Manely","year":"1959","journal-title":"Acta Zool"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib9","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0074-7696(04)40002-3","article-title":"Cellular distribution and functions of P2 receptor subtypes in different systems","volume":"240","author":"Burnstock","year":"2004","journal-title":"Int Rev Cytol"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib10","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1152\/physrev.00043.2006","article-title":"Physiology and pathophysiology of purinergic neurotransmission","volume":"87","author":"Burnstock","year":"2007","journal-title":"Physiol Rev"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib11","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/cm.970210305","article-title":"Reversible intracellular ATP changes in intact rat spermatozoa and effects on flagellar sperm movement","volume":"21","author":"Jeulin","year":"1992","journal-title":"Cell Motil Cytoskel"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib12","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S0015-0282(16)54974-7","article-title":"Adenosine triphosphate in semen and other sperm characteristics: their relevance for fertility prediction in men with normal sperm concentration","volume":"57","year":"1992","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib13","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1002\/j.1939-4640.1999.tb02517.x","article-title":"Rabbit spermatozoa: a model system for studying ATP homeostasis and motility","volume":"20","author":"Minelli","year":"1999","journal-title":"J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib14","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1095\/biolreprod.102.007419","article-title":"Adenosine triphosphate induces Ca2+ signal in epithelial cells of the mouse caput epididymis through activation of P2X and P2Y purinergic receptors","volume":"68","author":"Shariatmadari","year":"2003","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib15","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1111\/j.1476-5381.1988.tb11770.x","article-title":"Control of anion and fluid secretion by apical P2-purinoceptors in the rat epididymis","volume":"95","author":"Wong","year":"1988","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib16","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1093\/humrep\/14.3.694","article-title":"Sperm treatment with extracellular ATP increases fertilization rates in in-vitro fertilization for male factor infertility","volume":"14","author":"Rossato","year":"1999","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1159\/000047905","article-title":"Localisation of nucleotide P2X receptor subtypes during spermatogenesis in the adult rat testis","volume":"169","author":"Glass","year":"2001","journal-title":"Cells Tissue Organs"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib18","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0006-8993(92)90294-J","article-title":"Ultrastructural localisation of nitric oxide synthase immunoreactivity in guinea-pig enteric neurons","volume":"577","author":"Llewellyn-Smith","year":"1992","journal-title":"Brain Res"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0165-0270(93)90138-H","article-title":"The tungstate-stabilized tetramethylbenzidine reaction for light- and electron-microscopic immunocytochemistry and for revealing biocytin-filled neurons","volume":"46","author":"Llewellyn-Smith","year":"1993","journal-title":"J Neurosci Methods"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib20","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1530\/jrf.0.0810127","article-title":"Specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon","volume":"81","author":"Mortimer","year":"1987","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib21","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1095\/biolreprod.104.031252","article-title":"Sperm survival versus degradation in the mammalian epididymis: a hypothesis","volume":"71","author":"Jones","year":"2004","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1095\/biolreprod49.2.274","article-title":"Changes in movement characteristics of human spermatozoa along the length of the epididymis","volume":"49","author":"Yeung","year":"1993","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib23","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/j.1749-6632.1987.tb25107.x","article-title":"Motility and surface transformations of human spermatozoa during epididymal transit","volume":"513","author":"Dacheux","year":"1992","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1530\/jrf.0.0600291","article-title":"Evidence supporting the existence of sperm maturation in the human epididymis","volume":"60","author":"Hinrichsen","year":"1980","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib25","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0015-0282(16)49528-2","article-title":"The role of the human epididymis in sperm maturation and sperm storage as reflected in the consequences of epididymovasostomy","volume":"46","author":"Schoysman","year":"1986","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib26","first-page":"99","article-title":"Role of epididymal secretory proteins in sperm maturation with particular reference to the boar","volume":"53","author":"Dacheux","year":"1998","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib27","doi-asserted-by":"crossref","first-page":"19443","DOI":"10.1016\/S0021-9258(18)41795-4","article-title":"Extracellular ATP is a trigger for the acrosome reaction in human spermatozoa","volume":"267","author":"Foresta","year":"1992","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib28","doi-asserted-by":"crossref","first-page":"C1709","DOI":"10.1152\/ajpcell.1996.270.6.C1709","article-title":"Mechanism of human sperm activation by extracellular ATP","volume":"270","author":"Foresta","year":"1996","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0020-711X(83)90017-4","article-title":"ATP contents of human seminal plasma and isolated sperm at time intervals after ejaculation","volume":"15","author":"Singer","year":"1983","journal-title":"Int J Biochem"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib30","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/S0015-0282(16)49042-4","article-title":"The value of adenosine triphosphate (ATP) measurements in assessing the fertilizing ability of human spermatozoa","volume":"44","author":"Irvine","year":"1985","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib31","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/j.1365-2605.1989.tb01323.x","article-title":"Absence of correlation between the levels of ATP and other seminal compounds in semen and the results of human in-vitro fertilisation","volume":"12","author":"Mieusset","year":"1989","journal-title":"Int J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib32","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/j.1939-4640.1992.tb00320.x","volume":"13","author":"Vigue","year":"1992","journal-title":"J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/smns.1996.0023","article-title":"Neuronal synthesis, storage and release of ATP","volume":"8","author":"Sperl\u00e1gh","year":"1996","journal-title":"Semin Neurosci"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib34","first-page":"12","article-title":"ATP as a signaling molecule: the exocrine focus","volume":"18","author":"Novak","year":"2003","journal-title":"News Physiol Sci"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib35","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1111\/j.1365-2605.1987.tb00366.x","article-title":"Reassessment of the accuracy of traditional sperm characteristics and adenosine triphosphate (ATP) in estimating the fertilizing potential of human semen in vivo","volume":"10","author":"Comhaire","year":"1987","journal-title":"Int J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib36","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1071\/RD9900115","article-title":"Abnormal fluid transport by the epididymis as a cause of obstructive azoospermia","volume":"2","author":"Wong","year":"1990","journal-title":"Reprod Fertil Dev"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib37","series-title":"Epithelial secretion of water and electrolytes","first-page":"333","article-title":"Electrolyte and fluid transport in the epididymis","author":"Wong","year":"1990"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib38","first-page":"487","article-title":"The role of extracellular ATP in the male reproductive tract","volume":"59","author":"Zhou","year":"2007","journal-title":"Sheng Li Xue Bao"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1022857608849","article-title":"Extracellular purines from cells of seminiferous tubules","volume":"245","author":"Gelain","year":"2003","journal-title":"Mol Cell Biochem"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib40","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/j.1939-4640.1996.tb01760.x","article-title":"Changes in luminal fluid protein composition in the rat cauda epididymidis following partial sympathetic denervation","volume":"17","author":"Ricker","year":"1996","journal-title":"J Androl"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib41","series-title":"Handbook of experimental pharmacology","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/978-3-642-56921-0_6","article-title":"Purinergic signalling in the lower urinary tract","author":"Burnstock","year":"2001"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib42","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.fertnstert.2005.09.024","article-title":"The purinergic component of human vas deferens contraction","volume":"85","author":"Banks","year":"2006","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib43","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1476-5381.1991.tb12207.x","article-title":"Sympathetic co-transmission to the cauda epididymis of the rat: characterization of postjunctional adrenoceptors and purinoceptors","volume":"102","author":"Ventura","year":"1991","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib44","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/47495","article-title":"Reduced vas deferens contraction and male infertility in mice lacking P2X1 receptors","volume":"403","author":"Mulryan","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib45","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1095\/biolreprod32.2.399","article-title":"Surface changes in chimpanzee sperm during epididymal transit","volume":"32","author":"Young","year":"1985","journal-title":"Biol Reprod"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib46","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.anireprosci.2004.05.011","article-title":"Post-testicular sperm environment and fertility","volume":"82-83","author":"Gatti","year":"2004","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.fertnstert.2009.02.061_bib47","first-page":"R199","article-title":"Sperm motility-inhibiting factor in rat epididymis","volume":"242","author":"Turner","year":"1982","journal-title":"Am J Physiol"}],"container-title":["Fertility and Sterility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0015028209004889?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0015028209004889?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T07:38:09Z","timestamp":1758008289000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0015028209004889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":47,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["S0015028209004889"],"URL":"https:\/\/doi.org\/10.1016\/j.fertnstert.2009.02.061","relation":{},"ISSN":["0015-0282"],"issn-type":[{"value":"0015-0282","type":"print"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Changing P2X receptor localization on maturing sperm in the epididymides of mice, hamsters, rats, and humans: a preliminary study","name":"articletitle","label":"Article Title"},{"value":"Fertility and Sterility","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fertnstert.2009.02.061","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}