{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:08:04Z","timestamp":1791346084153,"version":"4.3.1"},"reference-count":46,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":1091,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"AML","award":["R14-021; R16-018"],"award-info":[{"award-number":["R14-021; R16-018"]}]},{"name":"Walking Egg non-profit organization"},{"name":"Belgian National Reference"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","fertstert.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fertility and Sterility"],"published-print":{"date-parts":[[2019,6]]},"DOI":"10.1016\/j.fertnstert.2019.02.002","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T19:47:57Z","timestamp":1555530477000},"page":"1135-1144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":58,"title":["Infectious human papillomavirus virions in semen reduce clinical pregnancy rates in women undergoing intrauterine insemination"],"prefix":"10.1016","volume":"111","author":[{"given":"Christophe Eric","family":"Depuydt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert Ghislain Gerard","family":"Donders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludo","family":"Verstraete","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davy","family":"Vanden Broeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan Frans Andre","family":"Beert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"Salembier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene","family":"Bosmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem","family":"Ombelet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fertnstert.2019.02.002_bib1","doi-asserted-by":"crossref","first-page":"230263","DOI":"10.1155\/2014\/230263","article-title":"Male and couple fertility impairment due to HPV-DNA sperm infection: update on molecular mechanism and clinical impact\u2014systematic review","volume":"2014","author":"Gizzo","year":"2014","journal-title":"Biomed Res Int"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib2","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4103\/aja.aja_77_17","article-title":"The risk of human papillomavirus infection for male fertility abnormality: a meta-analysis","volume":"20","author":"Xiong","year":"2018","journal-title":"Asian J Androl"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib3","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.fertnstert.2015.09.018","article-title":"Spontaneous fertility and in\u00a0vitro fertilization outcome: new evidence of human papillomavirus sperm infection","volume":"105","author":"Garolla","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib4","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1002\/cam4.473","article-title":"Serial type-specific human papillomavirus (HPV) load measurement allows differentiation between regressing cervical lesions and serial virion productive transient infections","volume":"4","author":"Depuydt","year":"2015","journal-title":"Cancer Med"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib5","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1111\/j.1582-4934.2012.01631.x","article-title":"Changes in type-specific human papillomavirus load predict progression to cervical cancer","volume":"16","author":"Depuydt","year":"2012","journal-title":"J Cell Mol Med"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib6","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1002\/ijc.30238","article-title":"Linear viral load increase of a single HPV-type in women with multiple HPV infections predicts progression to cervical cancer","volume":"139","author":"Depuydt","year":"2016","journal-title":"Int J Cancer"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib7","first-page":"211","article-title":"Human papillomavirus (HPV) virion induced cancer and subfertility, two sides of the same coin","volume":"8","author":"Depuydt","year":"2016","journal-title":"Facts Views Vis Obgyn"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.fertnstert.2012.09.006","article-title":"Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients","volume":"99","author":"Garolla","year":"2013","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib9","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.fertnstert.2008.10.050","article-title":"Human papillomavirus found in sperm head of young adult males affects the progressive motility","volume":"93","author":"Foresta","year":"2010","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib10","doi-asserted-by":"crossref","first-page":"e5847","DOI":"10.1371\/journal.pone.0005847","article-title":"Adsorption of human papillomavirus 16 to live human sperm","volume":"4","author":"Perez-Andino","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib11","doi-asserted-by":"crossref","first-page":"e18095","DOI":"10.1371\/journal.pone.0018095","article-title":"Identification of multiple HPV types on spermatozoa from human sperm donors","volume":"6","author":"Kaspersen","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib12","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1258\/0956462042395122","article-title":"Detection of high-risk HPV DNA in semen and its association with the quality of semen","volume":"15","author":"Rintala","year":"2004","journal-title":"Int J STD AIDS"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib13","doi-asserted-by":"crossref","first-page":"e15036","DOI":"10.1371\/journal.pone.0015036","article-title":"Mechanism of human papillomavirus binding to human spermatozoa and fertilizing ability of infected spermatozoa","volume":"6","author":"Foresta","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib14","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s10815-006-9030-8","article-title":"Human papilloma virus DNA exposure and embryo survival is stage-specific","volume":"23","author":"Henneberg","year":"2006","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib15","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1097\/CEJ.0000000000000241","article-title":"Serial measurement of type-specific human papillomavirus load enables classification of cervical intraepithelial neoplasia lesions according to occurring human papillomavirus-induced pathway","volume":"26","author":"Verhelst","year":"2017","journal-title":"Eur J Cancer Prev"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib16","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1023\/A:1007975005433","article-title":"Human papillomavirus is more prevalent in first trimester spontaneously aborted products of conception compared to elective specimens","volume":"14","author":"Hermonat","year":"1997","journal-title":"Virus Genes"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib17","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1016\/j.fertnstert.2010.11.047","article-title":"Human papillomavirus infection in couples undergoing in\u00a0vitro fertilization procedures: impact on reproductive outcomes","volume":"95","author":"Perino","year":"2011","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib18","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1111\/j.1600-0412.2011.01189.x","article-title":"Prevalence of human papillomavirus in spontaneously aborted products of conception","volume":"90","author":"Skoczynski","year":"2011","journal-title":"Acta Obstet Gynecol Scand"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib19","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1097\/AOG.0b013e3181c2a122","article-title":"Pregnancy outcomes from the pregnancy registry of a human papillomavirus type 6\/11\/16\/18 vaccine","volume":"114","author":"Dana","year":"2009","journal-title":"Obstet Gynecol"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib20","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.fertnstert.2003.08.020","article-title":"Possible role of bacterial and viral infections in miscarriages","volume":"81","author":"Matovina","year":"2004","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1093\/humupd\/dmp048","article-title":"World Health Organization reference values for human semen characteristics","volume":"16","author":"Cooper","year":"2010","journal-title":"Hum Reprod Update"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib22","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1093\/humrep\/12.7.1458","article-title":"Intrauterine insemination after ovarian stimulation with clomiphene citrate: predictive potential of inseminating motile count and sperm morphology","volume":"12","author":"Ombelet","year":"1997","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib23","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.rbmo.2017.01.016","article-title":"Predictive value of different covariates influencing pregnancy rate following intrauterine insemination with homologous semen: a prospective cohort study","volume":"34","author":"Thijssen","year":"2017","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib24","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.rbmo.2017.03.012","article-title":"Predictive factors influencing pregnancy rates after intrauterine insemination with frozen donor semen: a prospective cohort study","volume":"34","author":"Thijssen","year":"2017","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib25","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1093\/humrep\/dex234","article-title":"The international glossary on infertility and fertility care, 2017","volume":"32","author":"Zegers-Hochschild","year":"2017","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib26","series-title":"WHO laboratory manual for the examination and processing of human semen","year":"2010"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib27","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.bbrc.2007.01.169","article-title":"DNA damage reduces Taq DNA polymerase fidelity and PCR amplification efficiency","volume":"355","author":"Sikorsky","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib28","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.fertnstert.2005.11.027","article-title":"Estimation of human sperm gene-specific deoxyribonucleic acid damage by real-time polymerase chain reaction analysis","volume":"85","author":"San","year":"2006","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib29","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.1128\/JCM.01231-12","article-title":"Clinical validation of a type-specific real time quantitative human papillomavirus PCR to the performance of hybrid capture 2 for the purpose of cervical cancer screening","volume":"50","author":"Depuydt","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib30","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1515\/cclm.2011.835","article-title":"High-throughput detection, genotyping and quantification of the human papillomavirus using real-time PCR","volume":"50","author":"Micalessi","year":"2012","journal-title":"Clin Chem Lab Med"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib31","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1056\/NEJMoa021641","article-title":"Epidemiologic classification of human papillomavirus types associated with cervical cancer","volume":"348","author":"Munoz","year":"2003","journal-title":"N Engl J Med"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib32","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1365-2303.2006.00386.x","article-title":"Improved endocervical sampling and HPV viral load detection by Cervex-Brush Combi","volume":"17","author":"Depuydt","year":"2006","journal-title":"Cytopathology"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib33","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1111\/j.1582-4934.2007.00073.x","article-title":"Comparison of MY09\/11 consensus PCR and type-specific PCRs in the detection of oncogenic HPV types","volume":"11","author":"Depuydt","year":"2007","journal-title":"J Cell Mol Med"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib34","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0169-2607(95)01703-8","article-title":"MedCalc: a new computer program for medical statistics","volume":"48","author":"Schoonjans","year":"1995","journal-title":"Comput Methods Programs Biomed"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib35","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0015-0282(99)00012-6","article-title":"Influence of ovarian stimulation on the detection of human papillomavirus DNA in cervical scrapes obtained from patients undergoing assisted reproductive techniques","volume":"71","author":"Strehler","year":"1999","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib36","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/humrep\/det453","article-title":"Prevalence of human papillomaviruses in semen: a systematic review and meta-analysis","volume":"29","author":"Laprise","year":"2014","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib37","series-title":"Human papillomavirus and related diseases\u2014from bench to bedside\u2014a clinical perspective","first-page":"305","article-title":"Human papillomavirus in donor semen in Belgium","author":"D'Hauwers","year":"2012"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib38","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1159\/000482008","article-title":"The risk of human papillomavirus infection for spontaneous abortion, spontaneous preterm birth, and pregnancy rate of assisted reproductive technologies: a systematic review and meta-Analysis","volume":"83","author":"Xiong","year":"2018","journal-title":"Gynecol Obstet Invest"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib39","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1023\/A:1020458100382","article-title":"Tenacity of exogenous human papillomavirus DNA in sperm washing","volume":"16","author":"Brossfield","year":"1999","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib40","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/S0015-0282(16)56719-3","article-title":"Human papillomavirus gene sequences in washed human sperm deoxyribonucleic acid","volume":"61","author":"Chan","year":"1994","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib41","first-page":"357","article-title":"Human papillomavirus DNA detection in sperm using polymerase chain reaction","volume":"97","author":"Olatunbosun","year":"2001","journal-title":"Obstet Gynecol"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib42","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1038\/s41598-018-19369-z","article-title":"Human papillomavirus prophylactic vaccination improves reproductive outcome in infertile patients with HPV semen infection: a retrospective study","volume":"8","author":"Garolla","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib43","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S0140-6736(13)60809-0","article-title":"Incidence and clearance of oral human papillomavirus infection in men: the HIM cohort study","volume":"382","author":"Kreimer","year":"2013","journal-title":"Lancet"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib44","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1002\/ijc.27828","article-title":"Patterns of persistent genital human papillomavirus infection among women worldwide: a literature review and meta-analysis","volume":"133","author":"Rositch","year":"2013","journal-title":"Int J Cancer"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib45","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1016\/j.fertnstert.2009.11.012","article-title":"Clinical and prognostic significance of human papillomavirus DNA in the sperm or exfoliated cells of infertile patients and subjects with risk factors","volume":"94","author":"Foresta","year":"2010","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2019.02.002_bib46","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1186\/1471-2334-13-593","article-title":"Human papillomavirus proteins are found in peripheral blood and semen Cd20+ and Cd56+ cells during HPV-16 semen infection","volume":"13","author":"Foresta","year":"2013","journal-title":"BMC Infect Dis"}],"container-title":["Fertility and Sterility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0015028219300809?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0015028219300809?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T08:22:58Z","timestamp":1758010978000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0015028219300809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["S0015028219300809"],"URL":"https:\/\/doi.org\/10.1016\/j.fertnstert.2019.02.002","relation":{},"ISSN":["0015-0282"],"issn-type":[{"value":"0015-0282","type":"print"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Infectious human papillomavirus virions in semen reduce clinical pregnancy rates in women undergoing intrauterine insemination","name":"articletitle","label":"Article Title"},{"value":"Fertility and Sterility","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fertnstert.2019.02.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a92019 American Society for Reproductive Medicine, Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}