{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T02:13:16Z","timestamp":1788228796396,"version":"build-2803163510"},"reference-count":33,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100020400","name":"Cornell University Clinical and Translational Science Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020400","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007273","name":"Medical College, Weill Cornell Medicine","doi-asserted-by":"publisher","award":["UL1TR002384"],"award-info":[{"award-number":["UL1TR002384"]}],"id":[{"id":"10.13039\/100007273","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","fertstert.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fertility and Sterility"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.fertnstert.2025.04.036","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T19:09:32Z","timestamp":1746212972000},"page":"460-467","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fertilization and embryo development in fresh vs. vitrified oocytes with sperm from men with nonobstructive azoospermia"],"prefix":"10.1016","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6816-658X","authenticated-orcid":false,"given":"Christopher P.","family":"Moutos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjile","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zev","family":"Rosenwaks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rony T.","family":"Elias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter N.","family":"Schlegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fertnstert.2025.04.036_bib1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/S0022-5347(17)38662-7","article-title":"Evaluation of the azoospermic patient","volume":"142","author":"Jarow","year":"1989","journal-title":"J Urol"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41572-023-00459-w","article-title":"Male infertility","volume":"9","author":"Eisenberg","year":"2023","journal-title":"Nat Rev Dis Primers"},{"issue":"Suppl 1","key":"10.1016\/j.fertnstert.2025.04.036_bib3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.6061\/clinics\/2013(Sup01)03","article-title":"The epidemiology and etiology of azoospermia","volume":"68","author":"Cocuzza","year":"2013","journal-title":"Clinics (Sao Paulo)"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib4","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.fertnstert.2020.11.016","article-title":"Diagnosis and treatment of infertility in men: AUA\/ASRM guideline part II","volume":"115","author":"Schlegel","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib5","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1097\/JU.0000000000003894","article-title":"Timing of testicular biopsy in relation to oocyte retrieval and the outcomes of intracytoplasmic sperm injection","volume":"211","author":"Ng","year":"2024","journal-title":"J Urol"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib6","doi-asserted-by":"crossref","first-page":"663287","DOI":"10.3389\/fmed.2021.663287","article-title":"An eight year experience of autologous oocyte vitrification for infertile patients owing to unavailability of sperm on oocyte retrieval day","volume":"8","author":"Fu","year":"2021","journal-title":"Front Med (Lausanne)"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib7","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1007\/s10815-018-1206-5","article-title":"Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis","volume":"35","author":"Yu","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib8","doi-asserted-by":"crossref","first-page":"e113671","DOI":"10.1371\/journal.pone.0113671","article-title":"Intracytoplasmic sperm injection (ICSI) in extreme cases of male infertility","volume":"9","author":"Palermo","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib9","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0015-0282(03)00551-X","article-title":"High survival rate of vitrified human oocytes results in clinical pregnancy","volume":"80","author":"Katayama","year":"2003","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib10","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1016\/j.fertnstert.2008.08.069","article-title":"Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification","volume":"92","author":"Cao","year":"2009","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib11","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/S1472-6483(10)60766-3","article-title":"Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries","volume":"14","author":"Antinori","year":"2007","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib12","doi-asserted-by":"crossref","first-page":"103367","DOI":"10.1016\/j.rbmo.2023.103367","article-title":"Planned oocyte cryopreservation: the state of the ART","volume":"47","author":"Cascante","year":"2023","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib13","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/humrep\/14.1.131","article-title":"Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision","volume":"14","author":"Schlegel","year":"1999","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.theriogenology.2006.09.014","article-title":"Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method","volume":"67","author":"Kuwayama","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib15","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1007\/s10815-018-1183-8","article-title":"Azoospermia and embryo morphokinetics: testicular sperm-derived embryos exhibit delays in early cell cycle events and increased arrest prior to compaction","volume":"35","author":"Desai","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib16","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.fertnstert.2021.05.087","article-title":"Testicular sperm extraction vs. ejaculated sperm use for nonazoospermic male factor infertility","volume":"116","author":"Kendall","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib17","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/humrep\/16.1.125","article-title":"Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa","volume":"16","author":"Balaban","year":"2001","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib18","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/humupd\/dmm028","article-title":"Human oocyte cryopreservation","volume":"13","author":"Gook","year":"2007","journal-title":"Hum Reprod Update"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.fertnstert.2016.10.002","article-title":"Cryopreserved oocyte versus fresh oocyte assisted reproductive technology cycles, United States, 2013","volume":"107","author":"Crawford","year":"2017","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib20","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1007\/s10815-023-03016-2","article-title":"Similar pregnancy outcomes from fresh and frozen donor oocytes transferred to gestational carriers: a SART database analysis isolating the effects of oocyte vitrification","volume":"41","author":"Kloos","year":"2024","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib21","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.fertnstert.2013.04.040","article-title":"Oocyte efficiency: does live birth rate differ when analyzing cryopreserved and fresh oocytes on a per-oocyte basis?","volume":"100","author":"Goldman","year":"2013","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib22","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1007\/s10815-023-02901-0","article-title":"Embryo ploidy in vitrified versus fresh oocytes: is there a difference?","volume":"40","author":"Tsai","year":"2023","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib23","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1093\/humrep\/deq146","article-title":"Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial","volume":"25","author":"Cobo","year":"2010","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib24","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.fertnstert.2012.04.028","article-title":"Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting","volume":"98","author":"Forman","year":"2012","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib25","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1093\/humrep\/deaa178","article-title":"Efficiency and efficacy of vitrification in 35 654 sibling oocytes from donation cycles","volume":"35","author":"Cornet-Bartolom\u00e9","year":"2020","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib26","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fertnstert.2021.02.024","article-title":"Evidence-based outcomes after oocyte cryopreservation for donor oocyte in vitro fertilization and planned oocyte cryopreservation: a guideline","volume":"116","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib27","doi-asserted-by":"crossref","first-page":"5722","DOI":"10.3390\/jcm11195722","article-title":"Opportunities and limits of conventional IVF versus ICSI: it is time to come off the fence","volume":"11","author":"Balli","year":"2022","journal-title":"J Clin Med"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib28","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1007\/s10815-018-1234-1","article-title":"Clinical outcomes following ICSI cycles using surgically recovered sperm and the impact of maternal age: 2004-2015 SART CORS registry","volume":"35","author":"Mahesan","year":"2018","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib29","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1093\/humrep\/15.6.1383","article-title":"Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia","volume":"15","author":"De Croo","year":"2000","journal-title":"Hum Reprod"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib30","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S1472-6483(10)60072-7","article-title":"Live birth from vitrified-warmed human oocytes fertilized with frozen-thawed testicular spermatozoa","volume":"19","author":"Liow","year":"2009","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib31","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1016\/j.fertnstert.2010.06.080","article-title":"Pregnancies and deliveries after injection of vitrified-warmed oocytes with cryopreserved testicular sperm","volume":"94","author":"Selman","year":"2010","journal-title":"Fertil Steril"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib32","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3109\/19396368.2012.656797","article-title":"Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa","volume":"58","author":"Zeke","year":"2012","journal-title":"Syst Biol Reprod Med"},{"key":"10.1016\/j.fertnstert.2025.04.036_bib33","doi-asserted-by":"crossref","first-page":"e0224919","DOI":"10.1371\/journal.pone.0224919","article-title":"Failed sperm retrieval from severely oligospermic or non-obstructive azoospermic patients on oocyte retrieval day: emergent oocyte cryopreservation is a feasible strategy","volume":"14","author":"Lin","year":"2019","journal-title":"PLoS One"}],"container-title":["Fertility and Sterility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001502822500250X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001502822500250X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:16:27Z","timestamp":1788225387000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S001502822500250X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S001502822500250X"],"URL":"https:\/\/doi.org\/10.1016\/j.fertnstert.2025.04.036","relation":{},"ISSN":["0015-0282"],"issn-type":[{"value":"0015-0282","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Fertilization and embryo development in fresh vs. vitrified oocytes with sperm from men with nonobstructive azoospermia","name":"articletitle","label":"Article Title"},{"value":"Fertility and Sterility","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fertnstert.2025.04.036","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a92025 American Society for Reproductive Medicine, Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}