{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:23:15Z","timestamp":1789698195570,"version":"4.0.1"},"reference-count":43,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2011,7,29]],"date-time":"2011-07-29T00:00:00Z","timestamp":1311897600000},"content-version":"vor","delay-in-days":178,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fungal Genetics and Biology"],"published-print":{"date-parts":[[2011,2]]},"DOI":"10.1016\/j.fgb.2010.07.011","type":"journal-article","created":{"date-parts":[[2010,7,30]],"date-time":"2010-07-30T04:19:49Z","timestamp":1280463589000},"page":"192-199","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":47,"title":["The GATA-type transcriptional activator Gat1 regulates nitrogen uptake and metabolism in the human pathogen Cryptococcus neoformans"],"prefix":"10.1016","volume":"48","author":[{"given":"L\u00edvia","family":"Kmetzsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charley Christian","family":"Staats","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda L.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9bora L.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luna S.","family":"Joffe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rog\u00e9rio F.","family":"Louren\u00e7o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suely L.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Nimrichter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio L.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Schrank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilene Henning","family":"Vainstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fgb.2010.07.011_b0005","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/45287","article-title":"The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors","volume":"402","author":"Beck","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.fgb.2010.07.011_b0010","doi-asserted-by":"crossref","first-page":"35727","DOI":"10.1074\/jbc.M004235200","article-title":"Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases","volume":"275","author":"Bertram","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fgb.2010.07.011_b0015","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1101\/gad.13.24.3271","article-title":"The TOR signaling cascade regulates gene expression in response to nutrients","volume":"13","author":"Cardenas","year":"1999","journal-title":"Genes Dev."},{"key":"10.1016\/j.fgb.2010.07.011_b0020","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/j.1365-2958.2007.05676.x","article-title":"Sre1p, a regulator of oxygen sensing and sterol homeostasis, is required for virulence in Cryptococcus neoformans","volume":"64","author":"Chang","year":"2007","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fgb.2010.07.011_b0025","doi-asserted-by":"crossref","first-page":"5609","DOI":"10.1128\/jb.179.17.5609-5613.1997","article-title":"Nitrogen GATA factors participate in transcriptional regulation of vacuolar protease genes in Saccharomyces cerevisiae","volume":"179","author":"Coffman","year":"1997","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fgb.2010.07.011_b0030","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1128\/MCB.16.3.847","article-title":"Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae","volume":"16","author":"Coffman","year":"1996","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.fgb.2010.07.011_b0035","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1128\/IAI.68.2.443-448.2000","article-title":"Urease as a virulence factor in experimental cryptococcosis","volume":"68","author":"Cox","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fgb.2010.07.011_b0040","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1128\/AAC.45.11.3162-3170.2001","article-title":"Rapamycin and less immunosuppressive analogs are toxic to Candida albicans and Cryptococcus neoformans via FKBP12-dependent inhibition of TOR","volume":"45","author":"Cruz","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2010.07.011_b0045","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/BF00484090","article-title":"Nitrogen regulation of amino acid catabolism in Neurospora crassa","volume":"16","author":"Facklam","year":"1978","journal-title":"Biochem. Genet."},{"key":"10.1016\/j.fgb.2010.07.011_b0050","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.fgb.2009.03.003","article-title":"Structural and functional properties of the Trichosporon asahii glucuronoxylomannan","volume":"46","author":"Fonseca","year":"2009","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2010.07.011_b0055","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1128\/EC.2.5.1036-1045.2003","article-title":"Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. Gattii: implications for an outbreak on Vancouver Island, Canada","volume":"2","author":"Fraser","year":"2003","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0060","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.fgb.2007.11.001","article-title":"DelsGate, a robust and rapid gene deletion construction method","volume":"45","author":"Garcia-Pedrajas","year":"2008","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2010.07.011_b0065","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1128\/EC.3.2.385-392.2004","article-title":"Cryptococcus neoformans CAP59 (or Cap59p) is involved in the extracellular trafficking of capsular glucuronoxylomannan","volume":"3","author":"Garcia-Rivera","year":"2004","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0070","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1128\/IAI.00334-09","article-title":"Elucidating the pathogenesis of spores from the human fungal pathogen Cryptococcus neoformans","volume":"77","author":"Giles","year":"2009","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fgb.2010.07.011_b0075","doi-asserted-by":"crossref","first-page":"14866","DOI":"10.1073\/pnas.96.26.14866","article-title":"Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins","volume":"96","author":"Hardwick","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fgb.2010.07.011_b0080","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1007\/s004380050767","article-title":"The role of the Aspergillus fumigatus areA gene in invasive pulmonary aspergillosis","volume":"258","author":"Hensel","year":"1998","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.fgb.2010.07.011_b0085","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1385\/MB:12:1:35","article-title":"Nitrogen catabolite repression in Saccharomyces cerevisiae","volume":"12","author":"Hofman-Bang","year":"1999","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.fgb.2010.07.011_b0090","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1128\/EC.3.2.420-429.2004","article-title":"Cryptococcus neoformans virulence gene discovery through insertional mutagenesis","volume":"3","author":"Idnurm","year":"2004","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0095","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1093\/emboj\/18.10.2782","article-title":"Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast","volume":"18","author":"Jiang","year":"1999","journal-title":"EMBO J."},{"key":"10.1016\/j.fgb.2010.07.011_b0100","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1099\/mic.0.2008\/019729-0","article-title":"Threonine biosynthetic genes are essential in Cryptococcus neoformans","volume":"154","author":"Kingsbury","year":"2008","journal-title":"Microbiology"},{"key":"10.1016\/j.fgb.2010.07.011_b0105","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1099\/mic.0.26928-0","article-title":"Cryptococcus neoformans Ilv2p confers resistance to sulfometuron methyl and is required for survival at 37\u00b0C and in vivo","volume":"150","author":"Kingsbury","year":"2004","journal-title":"Microbiology"},{"key":"10.1016\/j.fgb.2010.07.011_b0110","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fgb.2010.07.011_b0115","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1111\/j.1365-2958.2007.05844.x","article-title":"Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans","volume":"65","author":"Lee","year":"2007","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fgb.2010.07.011_b0120","doi-asserted-by":"crossref","first-page":"14455","DOI":"10.1073\/pnas.97.26.14455","article-title":"Identification of the MATa mating-type locus of Cryptococcus neoformans reveals a serotype A MATa strain thought to have been extinct","volume":"97","author":"Lengeler","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fgb.2010.07.011_b0125","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.fgb.2007.08.006","article-title":"GLN3 encodes a global regulator of nitrogen metabolism and virulence of C. Albicans","volume":"45","author":"Liao","year":"2008","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2010.07.011_b0130","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1046\/j.1365-2958.2003.03747.x","article-title":"Nitrogen metabolism and virulence of Candida albicans require the GATA-type transcriptional activator encoded by GAT1","volume":"50","author":"Limjindaporn","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fgb.2010.07.011_b0135","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fgb.2010.07.011_b0140","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1119(02)00558-9","article-title":"Nitrogen regulation in Saccharomyces cerevisiae","volume":"290","author":"Magasanik","year":"2002","journal-title":"Gene"},{"key":"10.1016\/j.fgb.2010.07.011_b0145","first-page":"17","article-title":"Genetic regulation of nitrogen metabolism in the fungi","volume":"61","author":"Marzluf","year":"1997","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.fgb.2010.07.011_b0150","doi-asserted-by":"crossref","first-page":"e40","DOI":"10.1371\/journal.ppat.0010040","article-title":"Coping with multiple virulence factors: which is most important?","volume":"1","author":"McClelland","year":"2005","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fgb.2010.07.011_b0155","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1128\/EC.3.6.1601-1608.2004","article-title":"UGD1, encoding the Cryptococcus neoformans UDP-glucose dehydrogenase, is essential for growth at 37\u00b0C and for capsule biosynthesis","volume":"3","author":"Moyrand","year":"2004","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0160","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1111\/j.1365-2958.2008.06588.x","article-title":"Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans","volume":"71","author":"Panepinto","year":"2009","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fgb.2010.07.011_b0165","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1016\/j.febslet.2007.07.006","article-title":"Identification and characterization of an aspartyl protease from Cryptococcus neoformans","volume":"581","author":"Pinti","year":"2007","journal-title":"FEBS Lett."},{"key":"10.1016\/j.fgb.2010.07.011_b0170","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1128\/EC.00318-06","article-title":"Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport","volume":"6","author":"Rodrigues","year":"2007","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0175","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/EC.00079-07","article-title":"Amt2 permease is required to induce ammonium-responsive invasive growth and mating in Cryptococcus neoformans","volume":"7","author":"Rutherford","year":"2008","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.fgb.2010.07.011_b0180","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1111\/j.1567-1364.2006.00060.x","article-title":"Identification of direct and indirect targets of the Gln3 and Gat1 activators by transcriptional profiling in response to nitrogen availability in the short and long term","volume":"6","author":"Scherens","year":"2006","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.fgb.2010.07.011_b0185","doi-asserted-by":"crossref","first-page":"47724","DOI":"10.1074\/jbc.M307223200","article-title":"An alpha-1,3-mannosyltransferase of Cryptococcus neoformans","volume":"278","author":"Sommer","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fgb.2010.07.011_b0190","doi-asserted-by":"crossref","first-page":"9450","DOI":"10.1073\/pnas.92.21.9450","article-title":"Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes","volume":"92","author":"Stanbrough","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fgb.2010.07.011_b0195","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fgb.2010.07.011_b0200","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/S0955-0674(97)80078-6","article-title":"TOR signalling and control of cell growth","volume":"9","author":"Thomas","year":"1997","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.fgb.2010.07.011_b0205","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1128\/jb.175.5.1405-1411.1993","article-title":"Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA","volume":"175","author":"Toffaletti","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fgb.2010.07.011_b0210","doi-asserted-by":"crossref","first-page":"7327","DOI":"10.1073\/pnas.93.14.7327","article-title":"Dimorphism and haploid fruiting in Cryptococcus neoformans: association with the alpha-mating type","volume":"93","author":"Wickes","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fgb.2010.07.011_b0215","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0065-2164(09)01204-0","article-title":"The capsule of the fungal pathogen Cryptococcus neoformans","volume":"68","author":"Zaragoza","year":"2009","journal-title":"Adv. Appl. Microbiol."}],"container-title":["Fungal Genetics and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184510001465?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184510001465?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:39:44Z","timestamp":1761068384000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1087184510001465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["S1087184510001465"],"URL":"https:\/\/doi.org\/10.1016\/j.fgb.2010.07.011","relation":{},"ISSN":["1087-1845"],"issn-type":[{"value":"1087-1845","type":"print"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The GATA-type transcriptional activator Gat1 regulates nitrogen uptake and metabolism in the human pathogen Cryptococcus neoformans","name":"articletitle","label":"Article Title"},{"value":"Fungal Genetics and Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fgb.2010.07.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}