{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:23:54Z","timestamp":1790137434191,"version":"4.0.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/K017365\/1"],"award-info":[{"award-number":["BB\/K017365\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["097377"],"award-info":[{"award-number":["097377"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N006364\/1"],"award-info":[{"award-number":["MR\/N006364\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/M026663\/1"],"award-info":[{"award-number":["MR\/M026663\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000882","name":"University of Aberdeen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000882","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000360","name":"Scottish Funding Council","doi-asserted-by":"publisher","award":["BB\/M010996\/1"],"award-info":[{"award-number":["BB\/M010996\/1"]}],"id":[{"id":"10.13039\/501100000360","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fungal Genetics and Biology"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.fgb.2019.05.005","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T09:46:44Z","timestamp":1558604804000},"page":"98-106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"special_numbering":"C","title":["Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism"],"prefix":"10.1016","volume":"130","author":[{"given":"Eloise","family":"Ballard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem J.G.","family":"Melchers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seshu","family":"Tammireddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip D.","family":"Whitfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel A.","family":"Brakhage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alistair J.P.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Verweij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adilia","family":"Warris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fgb.2019.05.005_b0005","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s40694-018-0057-2","article-title":"Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus","volume":"5","author":"Al Abdallah","year":"2018","journal-title":"Fungal Biol. Biotechnol."},{"key":"10.1016\/j.fgb.2019.05.005_b0010","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00446-17","article-title":"A simple and universal system for gene manipulation in Aspergillus fumigatus: in vitro-assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates","volume":"2","author":"Al Abdallah","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.fgb.2019.05.005_b0015","doi-asserted-by":"crossref","first-page":"5113","DOI":"10.1128\/AAC.00517-11","article-title":"Interrogation of related clinical pan-azole-resistant Aspergillus fumigatus strains: G138C, Y431C, and G434C single nucleotide polymorphisms in cyp51A, upregulation of cyp51A, and integration and activation of transposon Atf1 in the cyp51A promoter","volume":"55","author":"Albarrag","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0020","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1006\/fgbi.1997.0980","article-title":"Autonomous Plasmid Replication in Aspergillus nidulans: AMA1 and MATE Elements","volume":"21","author":"Aleksenko","year":"1997","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2019.05.005_b0025","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.fgb.2019.05.005_b0030","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/JCM.37.10.3332-3337.1999","article-title":"Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans","volume":"37","author":"Arthington-Skaggs","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.fgb.2019.05.005_b0035","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0378-1119(85)90187-8","article-title":"Development of a high frequency transforming vector for Aspergillus nidulans","volume":"36","author":"Ballance","year":"1985","journal-title":"Gene"},{"key":"10.1016\/j.fgb.2019.05.005_b0040","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/0006-291X(83)91828-4","article-title":"Transformation of Aspergillus nidulans by the orotidine-5\u2019- phosphate decarboxylase gene of Neurospora crassa","volume":"112","author":"Ballance","year":"1983","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fgb.2019.05.005_b0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fgb.2018.02.003","article-title":"In-host microevolution of Aspergillus fumigatus: a phenotypic and genotypic analysis","author":"Ballard","year":"2018","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2019.05.005_b0050","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1002\/yea.1235","article-title":"Svf1 inhibits reactive oxygen species generation and promotes survival under conditions of oxidative stress in Saccharomyces cerevisiae","volume":"22","author":"Brace","year":"2005","journal-title":"Yeast"},{"key":"10.1016\/j.fgb.2019.05.005_b0055","doi-asserted-by":"crossref","first-page":"e0158724","DOI":"10.1371\/journal.pone.0158724","article-title":"Mitochondrial complex I Is a global regulator of secondary metabolism, virulence and azole sensitivity in fungi","author":"Bromley","year":"2016","journal-title":"Plos ONE"},{"key":"10.1016\/j.fgb.2019.05.005_b0060","doi-asserted-by":"crossref","first-page":"e50034","DOI":"10.1371\/journal.pone.0050034","article-title":"Discovery of a hapE mutation that causes azole resistance in Aspergillus fumigatus through whole genome sequencing and sexual crossing","volume":"7","author":"Camps","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.fgb.2019.05.005_b0065","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/jac\/dkh507","article-title":"Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma","volume":"55","author":"Chen","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0070","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1056\/NEJM197403142901104","article-title":"Defective superoxide production by granulocytes from patients with chronic granulomatous disease","volume":"290","author":"Curnutte","year":"1974","journal-title":"New Engl. J. Med."},{"key":"10.1016\/j.fgb.2019.05.005_b0075","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s00294-006-0073-2","article-title":"Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole","volume":"50","author":"da Silva Ferreira","year":"2006","journal-title":"Curr. Genet."},{"key":"10.1016\/j.fgb.2019.05.005_b0080","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.2217\/fmb.11.118","article-title":"Azole resistance in Aspergillus: a growing public health menace","volume":"6","author":"Denning","year":"2011","journal-title":"Future Microbiol."},{"key":"10.1016\/j.fgb.2019.05.005_b0085","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1128\/AAC.47.3.1120-1124.2003","article-title":"A Point Mutation in the 14\u03b1-Sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus","volume":"47","author":"Diaz-Guerra","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0090","first-page":"982","article-title":"Reference method for broth dilution antifungal susceptibility testing of filamentous fungi","volume":"14","author":"EUCAST","year":"2015","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.fgb.2019.05.005_b0095","doi-asserted-by":"crossref","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipids from animal tissues","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fgb.2019.05.005_b0100","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1093\/jac\/dkt075","article-title":"The cdr1B efflux transporter is associated with non-cyp51a-mediated itraconazole resistance in Aspergillus fumigatus","volume":"68","author":"Fraczek","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0105","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1128\/AAC.49.5.2119-2121.2005","article-title":"Differences in interactions between azole drugs related to modifications in the 14-\u03b1 sterol demethylase gene (cyp51A) of Aspergillus fumigatus","volume":"49","author":"Garcia-Effron","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0110","first-page":"1","article-title":"Sterol biosynthesis and azole tolerance is governed by the opposing actions of SrbA and the CCAAT binding complex","volume":"12","author":"Gsaller","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fgb.2019.05.005_b0115","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1107\/S1744309112051597","article-title":"Crystallization and preliminary X-ray crystallographic analysis of sterol transcription factor Upc2 from Saccharomyces cerevisiae","volume":"69","author":"Ha","year":"2013","journal-title":"Acta Crystallogr. Sect. F: Struct. Biol. Crystall. Commun."},{"key":"10.1016\/j.fgb.2019.05.005_b0120","first-page":"247","article-title":"The mechanism of nystatin action","volume":"378","author":"Harman","year":"1957","journal-title":"Irish J. Med. Sci."},{"key":"10.1016\/j.fgb.2019.05.005_b0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijantimicag.2006.08.017","article-title":"Multi-azole resistance in Aspergillus fumigatus","author":"Howard","year":"2006","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.fgb.2019.05.005_b0130","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1086\/524071","article-title":"Resistance to antifungal agents: mechanisms and clinical impact","volume":"46","author":"Kanafani","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.fgb.2019.05.005_b0135","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modelling, prediction, and analysis","volume":"10","author":"Kelly","year":"2015","journal-title":"Nat. Protocols"},{"key":"10.1016\/j.fgb.2019.05.005_b0140","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0014-5793(96)01360-9","article-title":"Resistance to fluconazole and cross-resistance to amphotericin B in Candida albicans from AIDS patients caused by defective sterol \u03945,6-desaturation","volume":"400","author":"Kelly","year":"1997","journal-title":"FEBS Lett."},{"key":"10.1016\/j.fgb.2019.05.005_b0145","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1128\/AAC.46.4.1046-1052.2002","article-title":"In vitro low-level resistance to azoles in Candida albicans is associated with changes in membrane lipid fluidity and asymmetry","volume":"46","author":"Kohli","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0150","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0378-1097(00)00071-9","article-title":"Phospholipid and sterol analysis of plasma membranes of azole-resistant Candida albicans strains","volume":"185","author":"L\u00f6ffler","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.fgb.2019.05.005_b0155","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S1471-4914(02)02280-3","article-title":"Molecular basis of resistance to azole antifungals","volume":"8","author":"Lupetti","year":"2002","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.fgb.2019.05.005_b0160","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1128\/AAC.47.2.577-581.2003","article-title":"Mutations in Aspergillus fumigatus resulting in reduced susceptibility to posaconazole appear to be restricted to a single amino acid in the cytochrome P450 14\u03b1-demethylase","volume":"47","author":"Mann","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0165","doi-asserted-by":"crossref","first-page":"D222","DOI":"10.1093\/nar\/gku1221","article-title":"CDD: NCBI\u2019s conserved domain database","volume":"43","author":"Marchler-Bauer","year":"2015","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.fgb.2019.05.005_b0170","doi-asserted-by":"crossref","first-page":"3402","DOI":"10.1128\/JCM.39.9.3402-3408.2001","article-title":"Colorimetric assay for antifungal susceptibility testing of Aspergillus species","volume":"39","author":"Meletiadis","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.fgb.2019.05.005_b0175","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1128\/AAC.48.7.2747-2750.2004","article-title":"Substitutions at methionine 220 in the 14\u03b1-sterol demethylase (cyp51A) of Aspergillus fumigatus are responsible for resistance in vitro to azole antifungal drugs","volume":"48","author":"Mellado","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0180","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/AAC.01092-06","article-title":"A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations","volume":"51","author":"Mellado","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0185","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1590\/S1517-83822008000200004","article-title":"Membrane fluidity and lipid composition of fluconazole resistant and susceptible strains of Candida albicans isolated from diabetic patients","volume":"39","author":"Mishra","year":"2008","journal-title":"Brazilian J. Microbiol."},{"key":"10.1016\/j.fgb.2019.05.005_b0190","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/molecules23051218","article-title":"Sterol composition of clinically relevant Mucorales and changes resulting from posaconazole treatment","volume":"23","author":"M\u00fcller","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.fgb.2019.05.005_b0195","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1128\/AAC.47.5.1719-1726.2003","article-title":"Multiple resistance mechanisms among Aspergillus fumigatus mutants with high-level resistance to itraconazole","volume":"47","author":"Nascimento","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0200","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0133085","article-title":"A CRISPR-Cas9 system for genetic engineering of filamentous fungi","volume":"10","author":"N\u00f8dvig","year":"2015","journal-title":"PLoS ONE"},{"key":"10.1016\/j.fgb.2019.05.005_b0205","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1080\/21505594.2017.1328343","article-title":"The Aspergillus fumigatus farnesyltransferase \u03b2-subunit, RamA, mediates growth, virulence, and antifungal susceptibility","volume":"8","author":"Norton","year":"2017","journal-title":"Virulence"},{"key":"10.1016\/j.fgb.2019.05.005_b0210","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"Ran","year":"2013","journal-title":"Nat. Protocols"},{"key":"10.1016\/j.fgb.2019.05.005_b0215","doi-asserted-by":"crossref","first-page":"e01755","DOI":"10.1128\/mBio.01755-18","article-title":"The multifunctional fungal ergosterol","volume":"9","author":"Rodrigues","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.fgb.2019.05.005_b0220","doi-asserted-by":"crossref","first-page":"e00437","DOI":"10.1128\/mBio.00437-19","article-title":"Mutations in hmg1, challenging the paradigm of clinical triazole resistance in Aspergillus fumigatus","volume":"10","author":"Rybak","year":"2019","journal-title":"mBio"},{"key":"10.1016\/j.fgb.2019.05.005_b0225","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S1043-6618(05)80047-7","article-title":"Pharmacology and mechanism of action of the new HMG-CoA reductase inhibitors","volume":"22","author":"Sirtori","year":"1990","journal-title":"Pharmacal. Res."},{"key":"10.1016\/j.fgb.2019.05.005_b0230","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S1087-1845(02)00016-6","article-title":"Increased expression of a novel Aspergillus fumigatus ABC transporter gene, atrF, in the presence of itraconazole in an itraconazole resistant clinical isolate","volume":"36","author":"Slaven","year":"2002","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2019.05.005_b0235","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1128\/AAC.01599-09","article-title":"Azole resistance profile of amino acid changes in Aspergillus fumigatus CYP51A based on protein homology modeling","volume":"54","author":"Snelders","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0240","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1371\/journal.pmed.0050219","article-title":"Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism","volume":"5","author":"Snelders","year":"2008","journal-title":"PLoS Med."},{"key":"10.1016\/j.fgb.2019.05.005_b0245","doi-asserted-by":"crossref","first-page":"e00894","DOI":"10.1128\/AAC.00894-18","article-title":"CRISPR\/Cas9 genome editing to demonstrate the contribution of Cyp51A Gly138Ser to azole resistance in Aspergillus fumigatus","volume":"62","author":"Umeyama","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2019.05.005_b0250","doi-asserted-by":"crossref","first-page":"e251","DOI":"10.1016\/S1473-3099(16)30138-4","article-title":"In-host adaptation and acquired triazole resistance in Aspergillus fumigatus: a dilemma for clinical management","volume":"16","author":"Verweij","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fgb.2019.05.005_b0255","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1021\/acssynbio.6b00203","article-title":"Functional reconstitution of a fungal natural product gene cluster by advanced genome editing","volume":"6","author":"Weber","year":"2016","journal-title":"ACS Synthet. Biol."},{"key":"10.1016\/j.fgb.2019.05.005_b0260","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s11046-009-9249-x","article-title":"Clinical itraconazole-resistant strains of Aspergillus fumigatus, isolated serially from a lung aspergilloma patient with pulmonary tuberculosis, can be detected with real-time PCR method","volume":"169","author":"Xu","year":"2010","journal-title":"Mycopathologia"},{"key":"10.1016\/j.fgb.2019.05.005_b0265","doi-asserted-by":"crossref","first-page":"6129","DOI":"10.1038\/ncomms7129","article-title":"Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2","volume":"6","author":"Yang","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fgb.2019.05.005_b0270","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mBio.00791-17","article-title":"A novel environmental azole resistance mutation in Aspergillus fumigatus and a possible role of sexual reproduction in its emergence","volume":"8","author":"Zhang","year":"2017","journal-title":"mBio"}],"container-title":["Fungal Genetics and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184519300751?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184519300751?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:33:59Z","timestamp":1778589239000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1087184519300751"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":54,"alternative-id":["S1087184519300751"],"URL":"https:\/\/doi.org\/10.1016\/j.fgb.2019.05.005","relation":{},"ISSN":["1087-1845"],"issn-type":[{"value":"1087-1845","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism","name":"articletitle","label":"Article Title"},{"value":"Fungal Genetics and Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fgb.2019.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}