{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:47:13Z","timestamp":1790621233632,"version":"4.1.0"},"reference-count":57,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fungal Genetics and Biology"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.fgb.2020.103365","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T11:53:27Z","timestamp":1583322807000},"page":"103365","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans"],"prefix":"10.1016","volume":"138","author":[{"given":"Garrick W.K.","family":"Spencer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheena M.H.","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paige E.","family":"Erpf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha S.I.","family":"Wizrah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor G.","family":"Dyba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas D.","family":"Condon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Fraser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fgb.2020.103365_b0005","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1128\/EC.05148-11","article-title":"The C2 domain protein Cts1 functions in the calcineurin signaling circuit during high-temperature stress responses in Cryptococcus neoformans","volume":"10","author":"Aboobakar","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.fgb.2020.103365_b0010","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1042\/BJ20060874","article-title":"Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging","volume":"400","author":"Ai","year":"2006","journal-title":"Biochem. J."},{"key":"10.1016\/j.fgb.2020.103365_b0015","doi-asserted-by":"crossref","first-page":"e0122916","DOI":"10.1371\/journal.pone.0122916","article-title":"A genomic safe haven for mutant complementation in Cryptococcus neoformans","volume":"10","author":"Arras","year":"2015","journal-title":"PLoS ONE"},{"key":"10.1016\/j.fgb.2020.103365_b0020","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0163049","article-title":"Chemical inhibitors of non-homologous end joining increase targeted construct integration in Cryptococcus neoformans","volume":"11","author":"Arras","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.fgb.2020.103365_b0025","doi-asserted-by":"crossref","first-page":"17918","DOI":"10.1038\/s41598-017-18106-2","article-title":"Convergent microevolution of Cryptococcus neoformans hypervirulence in the laboratory and the clinic","volume":"7","author":"Arras","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fgb.2020.103365_b0030","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1038\/nmeth.4045","article-title":"Fluorescent indicators for simultaneous reporting of all four cell cycle phases","volume":"13","author":"Bajar","year":"2016","journal-title":"Nat. Meth."},{"key":"10.1016\/j.fgb.2020.103365_b0035","doi-asserted-by":"crossref","first-page":"20889","DOI":"10.1038\/srep20889","article-title":"Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting","volume":"6","author":"Bajar","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fgb.2020.103365_b0040","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/laban0907-40","article-title":"Strategies to minimize background autofluorescence in live mice during noninvasive fluorescence optical imaging","volume":"36","author":"Bhaumik","year":"2007","journal-title":"Lab Anim. (NY)"},{"key":"10.1016\/j.fgb.2020.103365_b0045","doi-asserted-by":"crossref","first-page":"8979","DOI":"10.1093\/nar\/gks694","article-title":"Regulatory consequences of gene translocation in bacteria","volume":"40","author":"Block","year":"2012","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.fgb.2020.103365_b0050","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/fox037","article-title":"Designing microorganisms for heterologous biosynthesis of cannabinoids","volume":"17","author":"Carvalho","year":"2017","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.fgb.2020.103365_b0055","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.8303295","article-title":"Green fluorescent protein as a marker for gene expression","volume":"263","author":"Chalfie","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.fgb.2020.103365_b0060","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/JB.181.18.5636-5643.1999","article-title":"Isolation, characterization, and localization of a capsule-associated gene, CAP10, of Cryptococcus neoformans","volume":"181","author":"Chang","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fgb.2020.103365_b0065","doi-asserted-by":"crossref","first-page":"3682","DOI":"10.1128\/AAC.47.12.3682-3687.2003","article-title":"Improved green fluorescent protein reporter gene-based microplate screening for antituberculosis compounds by utilizing an acetamidase promoter","volume":"47","author":"Changsen","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.fgb.2020.103365_b0070","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1128\/IAI.68.2.443-448.2000","article-title":"Urease as a virulence factor in experimental cryptococcosis","volume":"68","author":"Cox","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fgb.2020.103365_b0075","series-title":"Methods in Cell Biology","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0091-679X(08)61946-9","article-title":"Chapter 2: Understanding Structure\u2014Function Relationships in the Aequorea victoria Green Fluorescent Protein","author":"Cubitt","year":"1998"},{"key":"10.1016\/j.fgb.2020.103365_b0080","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1128\/IAI.67.4.1812-1820.1999","article-title":"Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein","volume":"67","author":"del Poeta","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fgb.2020.103365_b0085","doi-asserted-by":"crossref","first-page":"103241","DOI":"10.1016\/j.fgb.2019.103241","article-title":"amdS as a dominant recyclable marker in Cryptococcus neoformans","author":"Erpf","year":"2019","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2020.103365_b0090","unstructured":"Foundation, T. N., 2008. The Nobel Prize in Chemsitry 2008, vol. 2017."},{"key":"10.1016\/j.fgb.2020.103365_b0095","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/ncomms1738","article-title":"Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%","volume":"3","author":"Goedhart","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fgb.2020.103365_b0100","doi-asserted-by":"crossref","first-page":"29188","DOI":"10.1074\/jbc.M102815200","article-title":"Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications","volume":"276","author":"Griesbeck","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fgb.2020.103365_b0105","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/373663b0","article-title":"Improved green fluorescence","volume":"373","author":"Heim","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.fgb.2020.103365_b0110","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1038\/emboj.2009.68","article-title":"A constitutively active GPCR governs morphogenic transitions in Cryptococcus neoformans","volume":"28","author":"Hsueh","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.fgb.2020.103365_b0115","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1002\/prot.22024","article-title":"Dark proteins: effect of inclusion body formation on quantification of protein expression","volume":"72","author":"Iafolla","year":"2008","journal-title":"Proteins"},{"key":"10.1016\/j.fgb.2020.103365_b0120","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1128\/EC.00214-06","article-title":"Peroxisome function regulates growth on glucose in the Basidiomycete fungus Cryptococcus neoformans","volume":"6","author":"Idnurm","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.fgb.2020.103365_b0125","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1099\/mic.0.039222-0","article-title":"Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi","volume":"156","author":"Idnurm","year":"2010","journal-title":"Microbiology"},{"key":"10.1016\/j.fgb.2020.103365_b0130","doi-asserted-by":"crossref","first-page":"e1004261","DOI":"10.1371\/journal.pgen.1004261","article-title":"Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation","volume":"10","author":"Janbon","year":"2014","journal-title":"PLoS Genet."},{"key":"10.1016\/j.fgb.2020.103365_b0135","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.fgb.2012.02.001","article-title":"Two cation transporters Ena1 and Nha1 cooperatively modulate ion homeostasis, antifungal drug resistance, and virulence of Cryptococcus neoformans via the HOG pathway","volume":"49","author":"Jung","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2020.103365_b0140","doi-asserted-by":"crossref","first-page":"35932","DOI":"10.1038\/srep35932","article-title":"Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity","volume":"6","author":"Kaishima","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fgb.2020.103365_b0145","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nmeth.2171","article-title":"Improving FRET dynamic range with bright green and red fluorescent proteins","volume":"9","author":"Lam","year":"2012","journal-title":"Nat. Meth."},{"key":"10.1016\/j.fgb.2020.103365_b0150","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/s41592-019-0352-8","article-title":"FPbase: a community-editable fluorescent protein database","volume":"16","author":"Lambert","year":"2019","journal-title":"Nat. Meth."},{"key":"10.1016\/j.fgb.2020.103365_b0155","article-title":"Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae","volume":"8","author":"Lee","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.fgb.2020.103365_b0160","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1128\/EC.00066-12","article-title":"Function of Cryptococcus neoformans KAR7 (SEC66) in karyogamy during unisexual and opposite-sex mating","volume":"11","author":"Lee","year":"2012","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.fgb.2020.103365_b0165","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1006\/bbrc.1999.0954","article-title":"Is green fluorescent protein toxic to the living cells?","volume":"260","author":"Liu","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fgb.2020.103365_b0170","doi-asserted-by":"crossref","first-page":"11999","DOI":"10.1038\/s41598-017-12212-x","article-title":"Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2","volume":"7","author":"Mastop","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fgb.2020.103365_b0175","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.bios.2013.04.046","article-title":"A multiplexed microfluidic platform for rapid antibiotic susceptibility testing","volume":"49","author":"Mohan","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.fgb.2020.103365_b0180","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1021\/bi00709a028","article-title":"Intermolecular energy transfer in the bioluminescent system of Aequorea","volume":"13","author":"Morise","year":"1974","journal-title":"Biochemistry"},{"key":"10.1016\/j.fgb.2020.103365_b0185","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1471-2148-7-119","article-title":"Positive selection for unpreferred codon usage in eukaryotic genomes","volume":"7","author":"Neafsey","year":"2007","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.fgb.2020.103365_b0190","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nbt1172","article-title":"Engineering and characterization of a superfolder green fluorescent protein","volume":"24","author":"Pedelacq","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fgb.2020.103365_b0195","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/S1473-3099(17)30243-8","article-title":"Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis","volume":"17","author":"Rajasingham","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fgb.2020.103365_b0200","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Meth."},{"key":"10.1016\/j.fgb.2020.103365_b0205","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/nbt1037","article-title":"Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein","volume":"22","author":"Shaner","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fgb.2020.103365_b0210","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nmeth.2413","article-title":"A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum","volume":"10","author":"Shaner","year":"2013","journal-title":"Nat. Meth."},{"key":"10.1016\/j.fgb.2020.103365_b0215","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.0022-2720.2005.01441.x","article-title":"The discovery of aequorin and green fluorescent protein","volume":"217","author":"Shimomura","year":"2005","journal-title":"J. Microsc."},{"key":"10.1016\/j.fgb.2020.103365_b0220","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/jcp.1030590302","article-title":"Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan","volume":"59","author":"Shimomura","year":"1962","journal-title":"Aequorea. J Cell Comp Physiol."},{"key":"10.1016\/j.fgb.2020.103365_b0225","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/yea.3065","article-title":"Plasmids for C-terminal tagging in Saccharomyces cerevisiae that contain improved GFP proteins, Envy and Ivy","volume":"32","author":"Slubowski","year":"2015","journal-title":"Yeast"},{"key":"10.1016\/j.fgb.2020.103365_b0230","doi-asserted-by":"crossref","first-page":"357","DOI":"10.4014\/jmb.1609.09036","article-title":"Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans","volume":"27","author":"So","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.fgb.2020.103365_b0235","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0022-2836(75)80083-0","article-title":"Detection of specific sequences among DNA fragments separated by gel electrophoresis","volume":"98","author":"Southern","year":"1975","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.fgb.2020.103365_b0240","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.fgb.2017.08.008","article-title":"A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome","volume":"108","author":"Upadhya","year":"2017","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.fgb.2020.103365_b0245","doi-asserted-by":"crossref","unstructured":"Vanherp, L., et al., 2019. Sensitive bioluminescence imaging of fungal dissemination to the brain in mouse models of cryptococcosis. dmm.039123.","DOI":"10.1242\/dmm.039123"},{"key":"10.1016\/j.fgb.2020.103365_b0250","doi-asserted-by":"crossref","first-page":"e15968","DOI":"10.1371\/journal.pone.0015968","article-title":"Automated analysis of cryptococcal macrophage parasitism using GFP-tagged cryptococci","volume":"5","author":"Voelz","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.fgb.2020.103365_b0255","article-title":"Studies of antibiotic resistance of beta-lactamase bacteria under different nutrition limitations at the single-cell level","volume":"10","author":"Wang","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.fgb.2020.103365_b0260","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00285-12","article-title":"Role of CTR4 in the Virulence of Cryptococcus neoformans","volume":"3","author":"Waterman","year":"2012","journal-title":"MBio"},{"key":"10.1016\/j.fgb.2020.103365_b0265","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1091\/mbc.e05-07-0699","article-title":"G protein-coupled receptor Gpr4 senses amino acids and activates the cAMP-PKA pathway in Cryptococcus neoformans","volume":"17","author":"Xue","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.fgb.2020.103365_b0270","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/yea.2895","article-title":"Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast","volume":"29","author":"Young","year":"2012","journal-title":"Yeast"},{"key":"10.1016\/j.fgb.2020.103365_b0275","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1128\/EC.00327-09","article-title":"A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans","volume":"9","author":"Zhang","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.fgb.2020.103365_b0280","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1006\/bbrc.1996.1573","article-title":"An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells","volume":"227","author":"Zhang","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fgb.2020.103365_b0285","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1128\/IAI.69.9.5589-5596.2001","article-title":"Laccase of Cryptococcus neoformans Is a Cell Wall-Associated Virulence Factor","volume":"69","author":"Zhu","year":"2001","journal-title":"Infect. Immun."}],"container-title":["Fungal Genetics and Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184520300566?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1087184520300566?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:43:48Z","timestamp":1761068628000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1087184520300566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":57,"alternative-id":["S1087184520300566"],"URL":"https:\/\/doi.org\/10.1016\/j.fgb.2020.103365","relation":{},"ISSN":["1087-1845"],"issn-type":[{"value":"1087-1845","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans","name":"articletitle","label":"Article Title"},{"value":"Fungal Genetics and Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fgb.2020.103365","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2020 Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103365"}}