{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:28:03Z","timestamp":1774927683100,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1009267"],"award-info":[{"award-number":["APP1009267"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fitoterapia"],"published-print":{"date-parts":[[2014,6]]},"DOI":"10.1016\/j.fitote.2014.02.016","type":"journal-article","created":{"date-parts":[[2014,3,6]],"date-time":"2014-03-06T08:45:40Z","timestamp":1394095540000},"page":"22-33","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":33,"special_numbering":"C","title":["A comparative study of extraction methods reveals preferred solvents for cystine knot peptide isolation from Momordica cochinchinensis seeds"],"prefix":"10.1016","volume":"95","author":[{"given":"Tunjung","family":"Mahatmanto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron G.","family":"Poth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua S.","family":"Mylne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Craik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fitote.2014.02.016_bb0005","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0300-9084(90)90067-Q","article-title":"Molecular recognition between serine proteases and new bioactive microproteins with a knotted structure","volume":"72","author":"Le Nguyen","year":"1990","journal-title":"Biochimie"},{"key":"10.1016\/j.fitote.2014.02.016_bb0010","doi-asserted-by":"crossref","first-page":"5965","DOI":"10.1021\/bi049711q","article-title":"Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: the importance of the cyclic cystine knot","volume":"43","author":"Colgrave","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.fitote.2014.02.016_bb0015","doi-asserted-by":"crossref","first-page":"7697","DOI":"10.1021\/jm800704e","article-title":"Engineering stabilized vascular endothelial growth factor-A antagonists: synthesis, structural characterization, and bioactivity of grafted analogues of cyclotides","volume":"51","author":"Gunasekera","year":"2008","journal-title":"J Med Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0020","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1039\/b801667d","article-title":"Chemical and biomimetic total syntheses of natural and engineered MCoTI cyclotides","volume":"6","author":"Thongyoo","year":"2008","journal-title":"Org Biomol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0025","doi-asserted-by":"crossref","first-page":"6197","DOI":"10.1021\/jm901233u","article-title":"Potent inhibitors of \u03b2-tryptase and human leukocyte elastase based on the MCoTI-II scaffold","volume":"52","author":"Thongyoo","year":"2009","journal-title":"J Med Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0030","doi-asserted-by":"crossref","first-page":"6709","DOI":"10.1182\/blood-2011-06-359141","article-title":"Engineering pro-angiogenic peptides using stable, disulfide-rich cyclic scaffolds","volume":"118","author":"Chan","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.fitote.2014.02.016_bb0035","doi-asserted-by":"crossref","first-page":"5718","DOI":"10.1002\/ange.201200984","article-title":"Orally active peptidic bradykinin B1 receptor antagonists engineered from a cyclotide scaffold for inflammatory pain treatment","volume":"124","author":"Wong","year":"2012","journal-title":"Angew Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0040","doi-asserted-by":"crossref","first-page":"40493","DOI":"10.1074\/jbc.M112.395442","article-title":"Design, synthesis, structural and functional characterization of novel melanocortin agonists based on the cyclotide kalata B1","volume":"287","author":"Eliasen","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0045","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1006\/jmbi.1999.3383","article-title":"Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif","volume":"294","author":"Craik","year":"1999","journal-title":"J Mol Biol"},{"key":"10.1016\/j.fitote.2014.02.016_bb0050","doi-asserted-by":"crossref","first-page":"D314","DOI":"10.1093\/nar\/gkm939","article-title":"KNOTTIN: the knottin or inhibitor cystine knot scaffold in 2007","volume":"36","author":"Gracy","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.fitote.2014.02.016_bb0055","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1002\/bip.21424","article-title":"Analysis and classification of circular proteins in CyBase","volume":"94","author":"Kaas","year":"2010","journal-title":"Biopolymers"},{"key":"10.1016\/j.fitote.2014.02.016_bb0060","doi-asserted-by":"crossref","first-page":"9337","DOI":"10.1021\/ja00099a064","article-title":"Circulins A and B: novel human immunodeficiency virus (HIV)-inhibitory macrocyclic peptides from the tropical tree Chassalia parvifolia","volume":"116","author":"Gustafson","year":"1994","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.fitote.2014.02.016_bb0065","doi-asserted-by":"crossref","first-page":"10614","DOI":"10.1073\/pnas.191366898","article-title":"Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis","volume":"98","author":"Jennings","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.fitote.2014.02.016_bb0070","doi-asserted-by":"crossref","first-page":"5495","DOI":"10.1016\/S0021-9258(20)79755-3","article-title":"Purification and properties of a carboxypeptidase inhibitor from potatoes","volume":"249","author":"Ryan","year":"1974","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0075","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0022-2836(82)90309-6","article-title":"Refined crystal-structure of the potato inhibitor complex of carboxypeptidase A at 2.5\u00c5 resolution","volume":"160","author":"Rees","year":"1982","journal-title":"J Mol Biol"},{"key":"10.1016\/j.fitote.2014.02.016_bb0080","doi-asserted-by":"crossref","first-page":"27033","DOI":"10.1074\/jbc.M112.370841","article-title":"Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae)","volume":"287","author":"Poth","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0085","first-page":"173","article-title":"An oxytocic principle found in Oldenlandia affinis DC","volume":"12","author":"Gran","year":"1970","journal-title":"Medd Nor Farm Selsk"},{"key":"10.1016\/j.fitote.2014.02.016_bb0090","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1105\/tpc.108.062331","article-title":"Distribution and evolution of circular miniproteins in flowering plants","volume":"20","author":"Gruber","year":"2008","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0095","doi-asserted-by":"crossref","first-page":"17598","DOI":"10.1074\/jbc.M111.338970","article-title":"Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities","volume":"287","author":"Nguyen","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0100","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/j.1399-3011.1989.tb00210.x","article-title":"Protease inhibitors from Ecballium elaterium seeds","volume":"33","author":"Favel","year":"1989","journal-title":"Int J Pept Protein Res"},{"key":"10.1016\/j.fitote.2014.02.016_bb0105","doi-asserted-by":"crossref","first-page":"5722","DOI":"10.1021\/bi9929756","article-title":"Squash trypsin inhibitors from Momordica cochinchinensis exhibit an atypical macrocyclic structure","volume":"39","author":"Hernandez","year":"2000","journal-title":"Biochemistry"},{"key":"10.1016\/j.fitote.2014.02.016_bb0110","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1021\/np900174n","article-title":"Isolation and characterization of peptides from Momordica cochinchinensis seeds","volume":"72","author":"Chan","year":"2009","journal-title":"J Nat Prod"},{"key":"10.1016\/j.fitote.2014.02.016_bb0115","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1105\/tpc.112.099085","article-title":"Cyclic peptides arising by evolutionary parallelism via asparaginyl-endopeptidase-mediated biosynthesis","volume":"24","author":"Mylne","year":"2012","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0120","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1021\/cb100388j","article-title":"Discovery of cyclotides in the Fabaceae plant family provides new insights into the cyclization, evolution, and distribution of circular proteins","volume":"6","author":"Poth","year":"2011","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.fitote.2014.02.016_bb0125","doi-asserted-by":"crossref","first-page":"24275","DOI":"10.1074\/jbc.M111.229922","article-title":"Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family","volume":"286","author":"Nguyen","year":"2011","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0130","doi-asserted-by":"crossref","first-page":"10127","DOI":"10.1073\/pnas.1103660108","article-title":"Discovery of an unusual biosynthetic origin for circular proteins in legumes","volume":"108","author":"Poth","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.fitote.2014.02.016_bb0135","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1021\/np9803878","article-title":"Seven novel macrocyclic polypeptides from Viola arvensis","volume":"62","author":"G\u00f6ransson","year":"1999","journal-title":"J Nat Prod"},{"key":"10.1016\/j.fitote.2014.02.016_bb0140","doi-asserted-by":"crossref","first-page":"46858","DOI":"10.1074\/jbc.M407421200","article-title":"Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins","volume":"279","author":"Dutton","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0145","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1105\/tpc.105.034678","article-title":"A continent of plant defense peptide diversity: cyclotides in Australian Hybanthus (Violaceae)","volume":"17","author":"Simonsen","year":"2005","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0150","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.1105\/tpc.104.021790","article-title":"Tissue-specific expression of head-to-tail cyclized miniproteins in Violaceae and structure determination of the root cyclotide Viola hederacea root cyclotide1","volume":"16","author":"Trabi","year":"2004","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0155","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1105\/tpc.106.042812","article-title":"Discovery of cyclotide-like protein sequences in graminaceous crop plants: ancestral precursors of circular proteins?","volume":"18","author":"Mulvenna","year":"2006","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0160","doi-asserted-by":"crossref","first-page":"3370","DOI":"10.1074\/jbc.M112.415356","article-title":"Discovery of linear cyclotides in monocot plant Panicum laxum of Poaceae family provides new insights into evolution and distribution of cyclotides in plants","volume":"288","author":"Nguyen","year":"2013","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0165","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1093\/jb\/117.2.432","article-title":"Trypsin and elastase inhibitors from bitter gourd (Momordica charantia LINN.) seeds: purification, amino acid sequences, and inhibitory activities of four new inhibitors","volume":"117","author":"Hamato","year":"1995","journal-title":"J Biochem (Tokyo)"},{"key":"10.1016\/j.fitote.2014.02.016_bb0170","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1105\/tpc.9.7.1055","article-title":"Seed germination and dormancy","volume":"9","author":"Bewley","year":"1997","journal-title":"Plant Cell"},{"key":"10.1016\/j.fitote.2014.02.016_bb0175","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1016\/j.biocel.2007.06.016","article-title":"The cyclic cystine knot miniprotein MCoTI-II is internalized into cells by macropinocytosis","volume":"39","author":"Greenwood","year":"2007","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1016\/j.fitote.2014.02.016_bb0180","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jconrel.2011.08.030","article-title":"Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways","volume":"155","author":"Contreras","year":"2011","journal-title":"J Control Release"},{"key":"10.1016\/j.fitote.2014.02.016_bb0185","doi-asserted-by":"crossref","first-page":"36932","DOI":"10.1074\/jbc.M111.264424","article-title":"Identification and characterization of a new family of cell-penetrating peptides: cyclic cell-penetrating peptides","volume":"286","author":"Cascales","year":"2011","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0190","series-title":"Plant proteomics: methods and protocols","first-page":"15","article-title":"Protein extraction from cereal seeds","author":"Branlard","year":"2007"},{"key":"10.1016\/j.fitote.2014.02.016_bb0195","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.ympev.2009.08.006","article-title":"A three-genome phylogeny of Momordica (Cucurbitaceae) suggests seven returns from dioecy to monoecy and recent long-distance dispersal to Asia","volume":"54","author":"Schaefer","year":"2010","journal-title":"Mol Phylogenet Evol"},{"key":"10.1016\/j.fitote.2014.02.016_bb0200","doi-asserted-by":"crossref","first-page":"22875","DOI":"10.1074\/jbc.M101666200","article-title":"Circular proteins in plants: solution structure of a novel macrocyclic trypsin inhibitor from Momordica cochinchinensis","volume":"276","author":"Felizmenio-Quimio","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0205","first-page":"174","article-title":"Oxytocic principles of Oldenlandia affinis","volume":"36","author":"Gran","year":"1973","journal-title":"Lloydia"},{"key":"10.1016\/j.fitote.2014.02.016_bb0210","first-page":"207","article-title":"Isolation of oxytocic peptides from Oldenlandia affinis by solvent-extraction of tetraphenylborate complexes and chromatography on sephadex LH-20","volume":"36","author":"Gran","year":"1973","journal-title":"Lloydia"},{"key":"10.1016\/j.fitote.2014.02.016_bb0215","doi-asserted-by":"crossref","first-page":"6904","DOI":"10.1021\/bi034043e","article-title":"A novel \u03b1-conotoxin identified by gene sequencing is active in suppressing the vascular response to selective stimulation of sensory nerves in vivo","volume":"42","author":"Sandall","year":"2003","journal-title":"Biochemistry"},{"key":"10.1016\/j.fitote.2014.02.016_bb0220","doi-asserted-by":"crossref","first-page":"23254","DOI":"10.1074\/jbc.M604550200","article-title":"The synthesis, structural characterization, and receptor specificity of the \u03b1-conotoxin Vc1.1","volume":"281","author":"Clark","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fitote.2014.02.016_bb0225","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.phytol.2012.09.001","article-title":"Optimization of cyclotide extraction parameters","volume":"5","author":"Yeshak","year":"2012","journal-title":"Phytochem Lett"}],"container-title":["Fitoterapia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0367326X14000616?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0367326X14000616?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,8,24]],"date-time":"2024-08-24T11:13:55Z","timestamp":1724498035000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0367326X14000616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":45,"alternative-id":["S0367326X14000616"],"URL":"https:\/\/doi.org\/10.1016\/j.fitote.2014.02.016","relation":{},"ISSN":["0367-326X"],"issn-type":[{"value":"0367-326X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comparative study of extraction methods reveals preferred solvents for cystine knot peptide isolation from Momordica cochinchinensis seeds","name":"articletitle","label":"Article Title"},{"value":"Fitoterapia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fitote.2014.02.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}