{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T06:08:41Z","timestamp":1793686121130,"version":"4.5.1"},"reference-count":85,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["15-CE34-0005"],"award-info":[{"award-number":["15-CE34-0005"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["16P05333"],"award-info":[{"award-number":["16P05333"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011891","name":"Universiti Malaysia Terengganu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011891","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018696","name":"R\u00e9gion Normandie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018696","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Food Microbiology"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.fm.2021.103870","type":"journal-article","created":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T02:46:52Z","timestamp":1626490012000},"page":"103870","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Evaluation of real-time qPCR-based methods to detect the DNA of the three protozoan parasites Cryptosporidium parvum, Giardia duodenalis and Toxoplasma gondii in the tissue and hemolymph of blue mussels (M. edulis)"],"prefix":"10.1016","volume":"102","author":[{"given":"Catherine","family":"Cazeaux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Lalle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Durand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Aubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Favennec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitender P.","family":"Dubey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Geffard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"La Carbona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fm.2021.103870_bib1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.fm.2014.05.012","article-title":"Detection of toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by real time PCR\/High-Resolution melting analysis (HRM)","volume":"44","author":"Aksoy","year":"2014","journal-title":"Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib2","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/S0020-7519(03)00181-4","article-title":"Molecular and bioassay-based detection of Toxoplasma gondii oocyst uptake by mussels (Mytilus galloprovincialis)","volume":"33","author":"Arkush","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib3","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1016\/j.watres.2011.10.013","article-title":"Waterborne transmission of protozoan parasites: review of worldwide outbreaks - an update 2004-2010","volume":"45","author":"Baldursson","year":"2011","journal-title":"Water Res."},{"key":"10.1016\/j.fm.2021.103870_bib4","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/jcm.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib5","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.tifs.2020.03.011","article-title":"Parasite detection in food: current status and future needs for validation","volume":"99","author":"Chalmers","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"10.1016\/j.fm.2021.103870_bib6","doi-asserted-by":"crossref","first-page":"851","DOI":"10.4269\/ajtmh.2006.75.851","article-title":"Cryptosporidium hominis: experimental challenge of healthy adults","volume":"75","author":"Chappell","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.fm.2021.103870_bib7","doi-asserted-by":"crossref","first-page":"7388","DOI":"10.1128\/AEM.00552-07","article-title":"Development of a method for detection of Giardia duodenalis cysts on lettuce and for simultaneous analysis of salad products for the presence of Giardia cysts and Cryptosporidium oocysts","volume":"73","author":"Cook","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib8","doi-asserted-by":"crossref","DOI":"10.1007\/s00436-018-5832-8","article-title":"First report of Toxoplasma gondii sporulated oocysts and Giardia duodenalis in commercial green-lipped mussels (Perna canaliculus) in New Zealand","author":"Coupe","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fm.2021.103870_bib9","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1007\/s00436-019-06357-z","article-title":"Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus)","volume":"118","author":"Coupe","year":"2019","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fm.2021.103870_bib10","doi-asserted-by":"crossref","first-page":"6910","DOI":"10.1128\/AEM.01027-07","article-title":"Maximizing recovery and detection of Cryptosporidium parvum oocysts from spiked eastern oyster (Crassostrea virginica) tissue samples","volume":"73","author":"Downey","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib11","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1186\/s13071-021-04769-4","article-title":"Outbreaks of clinical toxoplasmosis in humans: five decades of personal experience, perspectives and lessons learned","volume":"14","author":"Dubey","year":"2021","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.fm.2021.103870_bib12","series-title":"Toxoplasmosis of Animals and Humans","author":"Dubey","year":"2010"},{"key":"10.1016\/j.fm.2021.103870_bib13","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s00436-007-0573-0","article-title":"Detection of Toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts","volume":"101","author":"Dum\u00e8tre","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fm.2021.103870_bib14","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.mimet.2004.11.024","article-title":"Immunomagnetic separation of Toxoplasma gondii oocysts using a monoclonal antibody directed against the oocyst wall","volume":"61","author":"Dum\u00e8tre","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.fm.2021.103870_bib15","first-page":"6","article-title":"Comparison of automated","volume":"10","author":"Dundas","year":"2008","journal-title":"Nucleic Acid Extraction Methods with Manual Extraction"},{"key":"10.1016\/j.fm.2021.103870_bib16","doi-asserted-by":"crossref","first-page":"107809","DOI":"10.1016\/j.exppara.2019.107809","article-title":"Comparative evaluation of loop-mediated isothermal amplification (LAMP) vs qPCR for detection of Toxoplasma gondii oocysts DNA in mussels","volume":"208","author":"Durand","year":"2020","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib17","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.watres.2017.01.036","article-title":"Waterborne transmission of protozoan parasites: review of worldwide outbreaks - an update 2011-2016","volume":"114","author":"Efstratiou","year":"2017","journal-title":"Water Res."},{"key":"10.1016\/j.fm.2021.103870_bib18","doi-asserted-by":"crossref","first-page":"108904","DOI":"10.1016\/j.vetpar.2019.07.012","article-title":"A rapid and sensitive method to detect Toxoplasma gondii oocysts in soil samples","volume":"274","author":"Escotte-Binet","year":"2019","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.vetpar.2010.01.036","article-title":"Analysis of marine bivalve shellfish from the fish market in Santos city, S\u00e3o Paulo state, Brazil, for Toxoplasma gondii","volume":"170","author":"Esmerini","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib20","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1574-6968.2002.tb11318.x","article-title":"Development of a TaqMan quantitative PCR assay specific for Cryptosporidium parvum","volume":"214","author":"Fontaine","year":"2002","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.fm.2021.103870_bib21","doi-asserted-by":"crossref","first-page":"115297","DOI":"10.1016\/j.watres.2019.115297","article-title":"Use of the bivalve Dreissena polymorpha as a biomonitoring tool to reflect the protozoan load in freshwater bodies","volume":"170","author":"G\u00e9ba","year":"2020","journal-title":"Water Res."},{"key":"10.1016\/j.fm.2021.103870_bib22","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.marpolbul.2017.01.057","article-title":"First report of Tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators","volume":"117","author":"Ghozzi","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.fm.2021.103870_bib23","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.parint.2008.07.003","article-title":"Giardia and Cryptosporidium in inflowing water and harvested shellfish in a lagoon in Southern Italy","volume":"58","author":"Giangaspero","year":"2009","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.fm.2021.103870_bib24","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ijfoodmicro.2013.11.022","article-title":"Cryptosporidium parvum genotype IIa and Giardia duodenalis assemblage A in Mytilus galloprovincialis on sale at local food markets","volume":"171","author":"Giangaspero","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib25","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES2015.20.24.21160","article-title":"Waterborne outbreaks in the Nordic countries, 1998 to 2012","volume":"20","author":"Guzman-Herrador","year":"2015","journal-title":"Euro Surveill."},{"key":"10.1016\/j.fm.2021.103870_bib26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1051\/parasite\/2016013","article-title":"Giardia duodenalis genetic assemblages and hosts","volume":"23","author":"Heyworth","year":"2016","journal-title":"Parasite"},{"key":"10.1016\/j.fm.2021.103870_bib27","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fm.2016.01.002","article-title":"Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves","volume":"57","author":"Hohweyer","year":"2016","journal-title":"Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib28","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.4315\/0362-028X.JFP-13-002","article-title":"Tools and methods for detecting and characterizing giardia, cryptosporidium, and toxoplasma parasites in marine mollusks","volume":"76","author":"Hohweyer","year":"2013","journal-title":"J. Food Protect."},{"key":"10.1016\/j.fm.2021.103870_bib29","series-title":"Water Quality\u2013Isolation and Identification of Cryptosporidium Oocysts and Giardia Cysts from Water","year":"2006"},{"key":"10.1016\/j.fm.2021.103870_bib30","series-title":"Microbiology of the Food Chain - Method Validation - Part 1 - Vocabulary","year":"2016"},{"key":"10.1016\/j.fm.2021.103870_bib31","series-title":"Microbiology of the Food Chain - Method Validation - Part 2 - Protocol for the Validation of Alternative (Proprietary) Methods against a Reference Method","year":"2016"},{"key":"10.1016\/j.fm.2021.103870_bib32","series-title":"Microbiology of the Food Chain - Technical Requirements and Guidance on Establishment or Revision of a Standardized Reference Method","year":"2016"},{"key":"10.1016\/j.fm.2021.103870_bib33","series-title":"Microbiology of the Food Chain - Detection and Enumeration of Cryptosporidium and Giardia in Fresh Leafy Green Vegetables and Berry Fruits","year":"2016"},{"key":"10.1016\/j.fm.2021.103870_bib34","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1051\/parasite\/2013051","article-title":"Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases","volume":"20","author":"Jeddi","year":"2013","journal-title":"Parasite"},{"key":"10.1016\/j.fm.2021.103870_bib35","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1099\/jmm.0.2008\/001461-0","article-title":"Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays","volume":"57","author":"Jothikumar","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib36","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijfoodmicro.2019.02.011","article-title":"Comparison of Cryptosporidium oocyst recovery methods for their applicability for monitoring of consumer-ready fresh shellfish","volume":"296","author":"Kaupke","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib37","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1111\/jam.12999","article-title":"Zebra mussel as a new tool to show evidence of freshwater contamination by waterborne Toxoplasma gondii","volume":"120","author":"Kerambrun","year":"2016","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib38","article-title":"Public health risks associated with food-borne parasites","volume":"16","author":"Koutsoumanis","year":"2018","journal-title":"Scientific Opinion. EFSA Journal"},{"key":"10.1016\/j.fm.2021.103870_bib39","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fm.2017.10.001","article-title":"Loop-Mediated Isothermal Amplification-Lateral-Flow Dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad","volume":"70","author":"Lalle","year":"2018","journal-title":"Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib40","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.vetpar.2011.06.018","article-title":"Development of a sensitive method for Toxoplasma gondii oocyst extraction in soil","volume":"183","author":"L\u00e9lu","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib41","doi-asserted-by":"crossref","first-page":"5127","DOI":"10.1128\/AEM.00246-12","article-title":"Quantitative estimation of the viability of Toxoplasma gondii oocysts in soil","volume":"78","author":"L\u00e9lu","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib42","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1139\/W10-078","article-title":"Microbiological quality of blue mussels (Mytilus edulis) in Nunavik, Quebec: a pilot study","volume":"56","author":"L\u00e9vesque","year":"2010","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib43","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s11745-007-3078-9","article-title":"Seasonal variations of lipid content and composition in Perna viridis","volume":"42","author":"Li","year":"2007","journal-title":"Lipids"},{"key":"10.1016\/j.fm.2021.103870_bib44","doi-asserted-by":"crossref","first-page":"107848","DOI":"10.1016\/j.exppara.2020.107848","article-title":"Investigations from Northern Greece on mussels cultivated in areas proximal to wastewaters discharges, as a potential source for human infection with Giardia and Cryptosporidium","volume":"210","author":"Ligda","year":"2020","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib45","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ijfoodmicro.2019.03.009","article-title":"Protocol standardization for the detection of Giardia cysts and Cryptosporidium oocysts in Mediterranean mussels (Mytilus galloprovincialis)","volume":"298","author":"Ligda","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib46","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1645\/GE-1919.1","article-title":"Long-term survival of Toxoplasma gondii sporulated oocysts in seawater","volume":"95","author":"Lindsay","year":"2009","journal-title":"J. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib47","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1007\/s00436-008-1143-9","article-title":"Biomonitoring of surface and coastal water for Cryptosporidium, Giardia, and human-virulent microsporidia using molluscan shellfish","volume":"103","author":"Lucy","year":"2008","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fm.2021.103870_bib48","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.mimet.2004.10.017","article-title":"The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish","volume":"60","author":"MacRae","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.fm.2021.103870_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcp.2015.01.001","article-title":"Multiplex PCR for the detection and quantification of key zoonotic genotypes of Giardia, Cryptosporidium and Toxoplasma in wastewater and mussels","author":"Marangi","year":"2015","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.fm.2021.103870_bib50","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ijppaw.2016.01.004","article-title":"Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential","volume":"5","author":"Marin\u00e9 Oliveira","year":"2016","journal-title":"Int J Parasitol Parasites Wildl"},{"key":"10.1016\/j.fm.2021.103870_bib51","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1111\/are.13565","article-title":"Changes in proximate composition, lipid class and fatty acid profile in GreenshellTM mussels (Perna canaliculus) over an annual cycle","volume":"49","author":"Miller","year":"2018","journal-title":"Aquacult. Res."},{"key":"10.1016\/j.fm.2021.103870_bib52","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.mimet.2005.08.011","article-title":"Evaluation of methods for improved detection of Cryptosporidium spp. in mussels (Mytilus californianus)","volume":"65","author":"Miller","year":"2006","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.fm.2021.103870_bib53","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.fm.2017.08.008","article-title":"Interlaboratory validation of an improved method for detection of Cyclospora cayetanensis in produce using a real-time PCR assay","volume":"69","author":"Murphy","year":"2018","journal-title":"Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib54","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.4315\/0362-028X.JFP-16-492","article-title":"Evaluation of an improved U.S. Food and Drug administration method for the detection of Cyclospora cayetanensis in produce using real-time PCR","volume":"80","author":"Murphy","year":"2017","journal-title":"J. Food Protect."},{"key":"10.1016\/j.fm.2021.103870_bib55","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.4315\/JFP-20-121","article-title":"Multiplex quantitative PCR analysis of strawberries from bogot\u00e1, Colombia, for contamination with three parasites","volume":"83","author":"Ortiz Pineda","year":"2020","journal-title":"J. Food Protect."},{"key":"10.1016\/j.fm.2021.103870_bib56","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.marpolbul.2017.03.005","article-title":"Cryptosporidium species from common edible bivalves in Manila Bay, Philippines","volume":"119","author":"Pagoso","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.fm.2021.103870_bib57","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.watres.2013.09.017","article-title":"Bioaccumulation of human waterborne protozoa by zebra mussel (Dreissena polymorpha): interest for water biomonitoring","volume":"48","author":"Palos Ladeiro","year":"2014","journal-title":"Water Res."},{"key":"10.1016\/j.fm.2021.103870_bib58","doi-asserted-by":"crossref","DOI":"10.1007\/s11356-015-4296-y","article-title":"Assessment of Toxoplasma gondii levels in zebra mussel (Dreissena polymorpha) by real-time PCR: an organotropism study","author":"Palos Ladeiro","year":"2015","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.fm.2021.103870_bib59","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.3201\/eid2512.181565","article-title":"Patterns of transmission and sources of infection in outbreaks of human toxoplasmosis","volume":"25","author":"Pinto-Ferreira","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.fm.2021.103870_bib60","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.exppara.2010.09.007","article-title":"Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG)","volume":"127","author":"Putignani","year":"2011","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib61","series-title":"Waterborne Transmission of Giardiasis","first-page":"64","article-title":"The experimental transmission of Giardia lamblia among volunteer subjects","author":"Rendtorff","year":"1979"},{"key":"10.1016\/j.fm.2021.103870_bib62","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.fm.2021.103870_bib63","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ijfoodmicro.2007.07.058","article-title":"The potential for marine bivalve shellfish to act as transmission vehicles for outbreaks of protozoan infections in humans: a review","volume":"120","author":"Robertson","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib64","doi-asserted-by":"crossref","first-page":"959","DOI":"10.4315\/0362-028X-71.5.959","article-title":"Development and use of a pepsin digestion method for analysis of shellfish for Cryptosporidium oocysts and Giardia cysts","volume":"71","author":"Robertson","year":"2008","journal-title":"J. Food Protect.Parasites Vectors"},{"key":"10.1016\/j.fm.2021.103870_bib65","doi-asserted-by":"crossref","first-page":"107900","DOI":"10.1016\/j.exppara.2020.107900","article-title":"Why we need a European focus on foodborne parasites","volume":"214","author":"Robertson","year":"2020","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib66","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1051\/parasite\/2018009","article-title":"Assessing viability and infectivity of foodborne and waterborne stages (cysts\/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods","volume":"25","author":"Rousseau","year":"2018","journal-title":"Parasite"},{"key":"10.1016\/j.fm.2021.103870_bib67","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijpara.2017.09.004","article-title":"Foodborne cryptosporidiosis","volume":"48","author":"Ryan","year":"2018","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib68","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1111\/1750-3841.14338","article-title":"The challenges of DNA extraction in different assorted food matrices: a review","volume":"83","author":"Sajali","year":"2018","journal-title":"J. Food Sci."},{"key":"10.1016\/j.fm.2021.103870_bib69","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s00128-017-2223-2","article-title":"Cryptosporidium spp. contamination in Perna perna mussels destined for human consumption in southeastern Rio de Janeiro, Brazil","volume":"100","author":"Santos","year":"2018","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.fm.2021.103870_bib70","doi-asserted-by":"crossref","first-page":"103252","DOI":"10.1016\/j.fm.2019.103252","article-title":"Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay","volume":"84","author":"Shapiro","year":"2019","journal-title":"Food Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib71","doi-asserted-by":"crossref","first-page":"4177","DOI":"10.1111\/1462-2920.12685","article-title":"Surveillance for Toxoplasma gondii in California mussels (Mytilus californianus) reveals transmission of atypical genotypes from land to sea","volume":"17","author":"Shapiro","year":"2015","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.fm.2021.103870_bib72","first-page":"433","article-title":"Proteases in fish and shellfish: role on muscle softening and prevention","volume":"21","author":"Sriket","year":"2014","journal-title":"International Food Research Journal"},{"key":"10.1016\/j.fm.2021.103870_bib73","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1007\/s00436-015-4711-9","article-title":"The development and implementation of a method using blue mussels (Mytilus spp.) as biosentinels of Cryptosporidium spp. and Toxoplasma gondii contamination in marine aquatic environments","volume":"114","author":"Staggs","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fm.2021.103870_bib74","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1080\/10498850.2017.1346742","article-title":"Seasonal changes in quality and fatty acid composition of black mussel (Mytilus galloprovincialis)","volume":"26","author":"Stratev","year":"2017","journal-title":"J. Aquat. Food Prod. Technol."},{"key":"10.1016\/j.fm.2021.103870_bib75","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0020-7519(99)00033-8","article-title":"Long-term survival of Cryptosporidium parvum oocysts in seawater and in experimentally infected mussels (Mytilus galloprovincialis)","volume":"29","author":"Tamburrini","year":"1999","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib76","doi-asserted-by":"crossref","first-page":"535","DOI":"10.4315\/0362-028X.JFP-18-157","article-title":"Toxoplasma gondii and other zoonotic Protozoans in mediterranean mussel ( Mytilus galloprovincialis) and blue mussel ( Mytilus edulis): a food safety concern?","volume":"82","author":"Tedde","year":"2019","journal-title":"J. Food Protect."},{"key":"10.1016\/j.fm.2021.103870_bib77","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3390\/ijerph13040381","article-title":"Assessment and molecular characterization of human intestinal parasites in bivalves from orchard beach, NY, USA","volume":"13","author":"Tei","year":"2016","journal-title":"Int. J. Environ. Res. Publ. Health"},{"key":"10.1016\/j.fm.2021.103870_bib78","doi-asserted-by":"crossref","first-page":"108636","DOI":"10.1016\/j.foodres.2019.108636","article-title":"A novel multiplex real-time PCR for the detection of Echinococcus multilocularis, Toxoplasma gondii, and Cyclospora cayetanensis on berries","volume":"125","author":"Temesgen","year":"2019","journal-title":"Food Res. Int."},{"key":"10.1016\/j.fm.2021.103870_bib79","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.fm.2021.103870_bib80","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/0272-4332.00046","article-title":"Cryptosporidium dose-response studies: variation between hosts","volume":"22","author":"Teunis","year":"2002","journal-title":"Risk Anal."},{"key":"10.1016\/j.fm.2021.103870_bib81","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1001920","article-title":"World health organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis","volume":"12","author":"Torgerson","year":"2015","journal-title":"PLoS Med."},{"key":"10.1016\/j.fm.2021.103870_bib82","series-title":"Method 1623: Cryptosporidium and Giardia in Water by filtration\/IMS\/FA","year":"2005"},{"key":"10.1016\/j.fm.2021.103870_bib83","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.foodres.2015.05.010","article-title":"A reduced-cost approach for analyzing fresh produce for contamination with Cryptosporidium oocysts and\/or Giardia cysts","volume":"77","author":"Utaaker","year":"2015","journal-title":"Food Res. Int."},{"key":"10.1016\/j.fm.2021.103870_bib84","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0890-8508(03)00057-4","article-title":"Real-time PCR for the detection of Giardia lamblia","volume":"17","author":"Verweij","year":"2003","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.fm.2021.103870_bib85","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ijfoodmicro.2014.08.027","article-title":"Static tank depuration and chronic short-term experimental contamination of Eastern oysters (Crassostrea virginica) with Giardia duodenalis cysts","volume":"192","author":"Willis","year":"2015","journal-title":"Int. J. Food Microbiol."}],"container-title":["Food Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0740002021001350?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0740002021001350?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:24:17Z","timestamp":1777573457000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0740002021001350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":85,"alternative-id":["S0740002021001350"],"URL":"https:\/\/doi.org\/10.1016\/j.fm.2021.103870","relation":{},"ISSN":["0740-0020"],"issn-type":[{"value":"0740-0020","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of real-time qPCR-based methods to detect the DNA of the three protozoan parasites Cryptosporidium parvum, Giardia duodenalis and Toxoplasma gondii in the tissue and hemolymph of blue mussels (M. edulis)","name":"articletitle","label":"Article Title"},{"value":"Food Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fm.2021.103870","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103870"}}