{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:52:17Z","timestamp":1773301937014,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,7,20]],"date-time":"2012-07-20T00:00:00Z","timestamp":1342742400000},"content-version":"vor","delay-in-days":201,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":1339,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":1339,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>Rat small intestinal goblet cell mucins reacting with monoclonal antibody HCM31 increase significantly during regeneration from experimental mucosal damage and at the period of expulsion of parasitic nematode, <jats:italic>Nippostrongylus brasiliensis<\/jats:italic> (<jats:italic>N.b<\/jats:italic>). The reduction in reactivity of HCM31 with mucin upon neuraminidase treatment, suggested that HCM31 recognizes sialylated oligosaccharide on mucin. HCM31\u2010reactive sialomucins are therefore considered to play an important role in the physiological and pathological changes in the gastrointestinal mucosa. To determine the epitope for HCM31, oligosaccharide\u2013alditols reacted with HCM31 were obtained from the small intestinal mucins of <jats:italic>N.b<\/jats:italic>\u2010infected rats and purified by ion\u2010exchange chromatography followed by normal\u2010phase HPLC. Two HCM31\u2010reactive oligosaccharide\u2010alditols were obtained. Analyses using tandem mass spectrometry and NMR spectroscopy showed that these oligosaccharides were core 4 mucin\u2010type oligosaccharides having a common tetrasaccharide sequence, NeuAc\u03b12\u20103(GalNAc\u03b21\u20104)Gal\u03b21\u20104GlcNAc\u03b2\u2010 (Sd<jats:sup>a<\/jats:sup> blood group antigen). These structures were not found in the small intestinal mucin oligosaccharides from uninfected rats. This epitope specificity of HCM31 was also confirmed using previously established anti\u2010GM2 and anti\u2010Sd<jats:sup>a<\/jats:sup> antibodies. Taken together, these results strongly suggest that HCM31 specifically recognizes mucin\u2010type oligosaccharides with the Sd<jats:sup>a<\/jats:sup> tetrasaccharide sequence. Immunohistochemical examination of human gastrointestinal tracts showed that HCM31 site\u2010specifically stained the goblet cells in normal sigmoid colon and normal rectum, but the goblet cells stained with HCM31 were reduced in the corresponding cancer tissues. HCM31 seems to be useful for diagnosis of colonic cancer and for examining the function of secretory\u2010type mucin with Sd<jats:sup>a<\/jats:sup> antigen.<\/jats:p>","DOI":"10.1016\/j.fob.2012.07.006","type":"journal-article","created":{"date-parts":[[2012,7,20]],"date-time":"2012-07-20T20:35:19Z","timestamp":1342816519000},"page":"223-233","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["The monoclonal antibody HCM31 specifically recognises the Sd<sup>a<\/sup> tetrasaccharide in goblet cell mucin"],"prefix":"10.1002","volume":"2","author":[{"given":"Daigo","family":"Tsubokawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinobu","family":"Goso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rei","family":"Kawashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Ota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taeko","family":"Dohi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki I.","family":"Kawamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Ichikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Ishihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1251"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90379-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90380-1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(99)00186-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01886504"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040605"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1993.12.609"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00702350"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180409"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099250"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI59087"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.090501.080131"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.23.63"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520701579662"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-009-0007-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.08.008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9474-8_1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(92)90168-T"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0639"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2007.08.019"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3500805"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3580657"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.119"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39013-1"},{"key":"e_1_2_8_26_1","first-page":"6466","article-title":"Expression of UDP-GalNAc:NeuAc alpha 2,3Gal beta-R beta 1,4(GalNAc to Gal) N-acetylgalactosaminyltransferase involved in the synthesis of Sda antigen in human large intestine and colorectal carcinomas","volume":"49","author":"Malagolini N","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05731.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.8.4504"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(93)90037-5"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)93649-5"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006983125913"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(93)90213-O"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290070712"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960715)10:9<1027::AID-RCM634>3.0.CO;2-Y"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(03)01012-7"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01082444"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01049915"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.fob.2012.07.006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.fob.2012.07.006","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.fob.2012.07.006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T17:41:07Z","timestamp":1694972467000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.fob.2012.07.006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1016\/j.fob.2012.07.006"],"URL":"https:\/\/doi.org\/10.1016\/j.fob.2012.07.006","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The monoclonal antibody HCM31 specifically recognises the Sda tetrasaccharide in goblet cell mucin","name":"articletitle","label":"Article Title"},{"value":"FEBS Open Bio","name":"journaltitle","label":"Journal Title"},{"value":"http:\/\/dx.doi.org\/10.1016\/j.fob.2012.07.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Federation of European Biochemical Societies. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}