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The majority of these effects are mediated by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> generated during the catalytic conversion of <jats:sc>l<\/jats:sc>\u2010amino acids. The substrate analog <jats:sc>l<\/jats:sc>\u2010propargylglycine (LPG) irreversibly inhibited the enzyme from <jats:italic>Crotalus adamanteus<\/jats:italic> and <jats:italic>Crotalus atrox<\/jats:italic> in a dose\u2010 and time\u2010dependent manner. Inactivation was irreversible which was significantly protected by the substrate <jats:sc>l<\/jats:sc>\u2010phenylalanine. A Kitz\u2013Wilson replot of the inhibition kinetics suggested formation of reversible enzyme\u2013LPG complex, which occurred prior to modification and inactivation of the enzyme. UV\u2013visible and fluorescence spectra of the enzyme and the cofactor strongly suggested formation of covalent adduct between LPG and an active site residue of the enzyme. A molecular modeling study revealed that the FAD\u2010binding, substrate\u2010binding and the helical domains are conserved in SV\u2010LAAOs and both His223 and Arg322 are the important active site residues that are likely to get modified by LPG. Chymotrypsin digest of the LPG inactivated enzyme followed by RP\u2010HPLC and MALDI mass analysis identified His223 as the site of modification. The findings reported here contribute towards complete inactivation of SV\u2010LAAO as a part of snake envenomation management.<\/jats:p>","DOI":"10.1016\/j.fob.2013.01.010","type":"journal-article","created":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T17:46:38Z","timestamp":1359999998000},"page":"135-143","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Irreversible inactivation of snake venom <scp>l<\/scp>\u2010amino acid oxidase by covalent modification during catalysis of <scp>l<\/scp>\u2010propargylglycine"],"prefix":"10.1002","volume":"3","author":[{"given":"Jyotirmoy","family":"Mitra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasish","family":"Bhattacharyya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,4]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"1992 CRC Press Boca Raton FL B. 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