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The egg coat plays important roles in fertilization, especially in species\u2010specific interactions with sperm to induce the sperm acrosome reaction and to form the block to polyspermy. It is suggested that the physiological functions of the egg coat are mediated and\/or regulated coordinately by peptide and carbohydrate moieties of the ZP glycoproteins that are spatially arranged in the egg coat, whereas a comprehensive understanding of the architecture of vertebrate egg\u2010coat matrix remains elusive. Here, we deduced the orientations and\/or distributions of chicken ZP glycoproteins, ZP1, ZP3 and ZPD, in the egg\u2010coat matrix by confocal immunofluorescent microscopy, and in the ZP1\u2013ZP3 complexes generated<jats:italic>in vitro<\/jats:italic> by co\u2010immunoprecipitation assays. We further confirmed interdomain interactions of the ZP glycoproteins by far\u2010Western blot analyses of the egg\u2010coat proteins and pull\u2010down assays of ZP1 in the serum, using recombinant domains of ZP glycoproteins as probes. Our results suggest that the ZP1 and ZP3 bind through their ZP\u2010C domains to form the ZP1\u2013ZP3 complexes and fibrils, which are assembled into bundles through interactions between the repeat domains of ZP1 to form the ZP1\u2013ZP3 matrix, and that the ZPD molecules self\u2010associate and bind to the ZP1\u2013ZP3 matrix through its ZP\u2010N and ZP\u2010C domains to form the egg\u2010coat matrix. Based on these results, we propose a tentative model for the architecture of the chicken egg\u2010coat matrix that might be applicable to other vertebrate ones.<\/jats:p>","DOI":"10.1016\/j.fob.2015.05.005","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T08:42:29Z","timestamp":1432716149000},"page":"454-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Identification of distinctive interdomain interactions among ZP\u2010N, ZP\u2010C and other domains of zona pellucida glycoproteins underlying association of chicken egg\u2010coat matrix"],"prefix":"10.1002","volume":"5","author":[{"given":"Hiroki","family":"Okumura","sequence":"first","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture, Meijo University, Nagoya 468-8502, 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