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COX\u20102 is a mediator in inflammatory action, pain and some catabolic reactions in inflamed tissues. Here, we demonstrated a direct relationship between oxidative stress and Cox\u20102 expression in the bovine synovial fibroblasts. Furthermore, we elucidated a novel mechanism, in which oxidative stress induced phosphorylation of MAPKs and NF\u2010\u03baB through TAK1 activation and resulted in increased Cox\u20102 and prostaglandin E2 expression. Finally, we demonstrated that ROS\u2010induced Cox\u20102 expression was inhibited by supplementation of an antioxidant such as N\u2010acetyl cysteamine and hyaluronic acid<jats:italic>in vitro<\/jats:italic> and<jats:italic>in vivo<\/jats:italic>. From these results, we conclude that oxidative stress is an important factor for generation of Cox\u20102 in synovial fibroblasts and thus its neutralization may be an effective strategy in palliative therapy for chronic joint diseases.<\/jats:p>","DOI":"10.1016\/j.fob.2015.06.001","type":"journal-article","created":{"date-parts":[[2015,6,7]],"date-time":"2015-06-07T00:53:58Z","timestamp":1433638438000},"page":"492-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":137,"title":["Reactive oxygen species induce Cox\u20102 expression via TAK1 activation in synovial fibroblast cells"],"prefix":"10.1002","volume":"5","author":[{"given":"Yuta","family":"Onodera","sequence":"first","affiliation":[{"name":"Division of Cell Biology for Regenerative Medicine, Institute of Advanced Clinical Medicine, Kindai University Faculty of Medicine, Osaka, 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