{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T04:03:51Z","timestamp":1750737831639,"version":"3.41.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T00:00:00Z","timestamp":1739577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009570","name":"Srinakharinwirot University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007280","name":"Srinakharinwirot University","doi-asserted-by":"publisher","award":["413\/2565"],"award-info":[{"award-number":["413\/2565"]}],"id":[{"id":"10.13039\/501100007280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Food Chemistry: Molecular Sciences"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.fochms.2025.100252","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T08:29:23Z","timestamp":1739953763000},"page":"100252","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Novel SCAR markers for accurate diagnosis of toxic Chlorophyllum molybdites and C. globosum"],"prefix":"10.1016","volume":"10","author":[{"given":"Sittiporn","family":"Parnmen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onanong","family":"Pringsulaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sutheewan","family":"Binchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khwanruan","family":"Naksuwankul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achariya","family":"Rangsiruji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fochms.2025.100252_bb0005","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/5773275","article-title":"Possibilities of using Macrolepiota procera in the production of prohealth food and in medicine","volume":"2022","author":"Adamska","year":"2022","journal-title":"International Journal of Food Science"},{"issue":"10","key":"10.1016\/j.fochms.2025.100252_bb0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2021.e08093","article-title":"Basic concepts and methodologies of DNA marker systems in plant molecular breeding","volume":"7","author":"Amiteye","year":"2021","journal-title":"Heliyon"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0015","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12866-017-0958-x","article-title":"Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)","volume":"17","author":"Badotti","year":"2017","journal-title":"BMC Microbiology"},{"issue":"4","key":"10.1016\/j.fochms.2025.100252_bb0020","first-page":"477","article-title":"Mushroom poisoning by Chlorophyllum molybdites in Kerala","volume":"54","author":"Bijeesh","year":"2017","journal-title":"Journal of Mycopathological Research"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0025","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.sjbs.2015.09.002","article-title":"Occurrence and HAT-RAPD analysis of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand","volume":"24","author":"Butboonchoo","year":"2017","journal-title":"Saudi Journal of Biological Sciences"},{"issue":"4","key":"10.1016\/j.fochms.2025.100252_bb0030","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1111\/jam.14392","article-title":"SCAR marker for Phytophthora nicotianae and a multiplex PCR assay for simultaneous detection of P. nicotianae and Candidatus Liberibacter asiaticus in citrus","volume":"127","author":"Das","year":"2019","journal-title":"Journal of Applied Microbiology"},{"issue":"5","key":"10.1016\/j.fochms.2025.100252_bb0035","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.3390\/microorganisms11051158","article-title":"Nutritional value and antioxidant, antimicrobial and cytotoxic activity of wild macrofungi","volume":"11","author":"D\u00e1vila Giraldo","year":"2023","journal-title":"Microorganisms"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0040","doi-asserted-by":"crossref","DOI":"10.55674\/cs.v15i2.250017","article-title":"Evaluation of genetic variation in longan (Dimocarpus longan Lour) by high annealing temperature random amplified polymorphic DNA (HAT\u2212RAPD)","volume":"15","author":"Dum-Ampai","year":"2023","journal-title":"Creative Science"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0045","doi-asserted-by":"crossref","first-page":"113","DOI":"10.11646\/phytotaxa.451.2.2","article-title":"Lepiotaceous fungi of West Bengal, India: The genus Chlorophyllum","volume":"451","author":"Dutta","year":"2020","journal-title":"Phytotaxa"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0050","doi-asserted-by":"crossref","first-page":"6968","DOI":"10.1038\/s41467-022-34630-w","article-title":"Muscle5: High-accuracy alignment ensembles enable unbiased assessments of sequence homology and phylogeny","volume":"13","author":"Edgar","year":"2022","journal-title":"Nature Communications"},{"key":"10.1016\/j.fochms.2025.100252_bb0055","article-title":"Rapid identification of Amanita citrinoannulata poisoning using colorimetric and real-time fluorescence and loop-mediated isothermal amplification (LAMP) based on the nuclear ITS region","volume":"4","author":"Gao","year":"2022","journal-title":"Food Chemistry: Molecular Sciences"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0060","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1111\/j.1365-294X.1993.tb00005.x","article-title":"ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts","volume":"2","author":"Gardes","year":"1993","journal-title":"Molecular Ecology"},{"issue":"8","key":"10.1016\/j.fochms.2025.100252_bb0065","doi-asserted-by":"crossref","first-page":"848","DOI":"10.3390\/jof8080848","article-title":"Structural features and immunomodulatory effects of water-extractable polysaccharides from Macrolepiota procera (Scop.)","volume":"8","author":"Georgiev","year":"2022","journal-title":"Singer. Journal of fungi"},{"issue":"4","key":"10.1016\/j.fochms.2025.100252_bb0070","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.foodres.2011.03.006","article-title":"Effects of trophism on nutritional and nutraceutical potential of wild edible mushrooms","volume":"44","author":"Grangeia","year":"2011","journal-title":"Food Research International"},{"key":"10.1016\/j.fochms.2025.100252_bb0075","article-title":"OligoCalc: An online oligonucleotide properties calculator","volume":"35(Web Server issue), W43\u2013W46","author":"Kibbe","year":"2007","journal-title":"Nucleic Acids Research"},{"issue":"23","key":"10.1016\/j.fochms.2025.100252_bb0080","first-page":"3988","article-title":"Development of pollen parent cultivar-specific SCAR markers and a multiplex SCAR-PCR system for discrimination between pollen parent and seed parent in citrus","volume":"12","author":"Kim","year":"2023","journal-title":"Plants (Basel)"},{"issue":"8","key":"10.1016\/j.fochms.2025.100252_bb0085","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/j.fitote.2010.08.002","article-title":"SCAR markers: A potential tool for authentication of herbal drugs","volume":"81","author":"Kiran","year":"2010","journal-title":"Fitoterapia"},{"issue":"20","key":"10.1016\/j.fochms.2025.100252_bb0090","doi-asserted-by":"crossref","first-page":"3240","DOI":"10.3390\/foods11203240","article-title":"Biochemical and molecular profiling of wild edible mushrooms from Huila, Angola","volume":"11","author":"Kissanga","year":"2022","journal-title":"Foods"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0095","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3136\/fstr.20.225","article-title":"Development of a human gastric digestion simulator equipped with peristalsis function for the direct observation and analysis of the food digestion process","volume":"20","author":"Kozu","year":"2014","journal-title":"Food Science and Technology Research"},{"key":"10.1016\/j.fochms.2025.100252_bb0100","first-page":"41","article-title":"DNA-based identification of gastrointestinal irritant mushrooms in the genus Chlorophyllum: A food poisoning case in Thailand","volume":"31","author":"Leudang","year":"2017","journal-title":"Journal of Health Research"},{"issue":"3","key":"10.1016\/j.fochms.2025.100252_bb0105","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1080\/00275514.2023.2177471","article-title":"The epidemiology of wild mushroom poisoning in Israel","volume":"115","author":"Lewinsohn","year":"2023","journal-title":"Mycologia"},{"issue":"4","key":"10.1016\/j.fochms.2025.100252_bb0110","doi-asserted-by":"crossref","first-page":"64","DOI":"10.46234\/ccdcw2024.014","article-title":"Mushroom poisoning outbreaks - China, 2023","volume":"6","author":"Li","year":"2024","journal-title":"China CDC weekly"},{"issue":"8","key":"10.1016\/j.fochms.2025.100252_bb0115","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1007\/s13205-020-02325-y","article-title":"SCAR marker for identification and discrimination of specific medicinal Lycium chinense Miller from Lycium species from ramp-PCR RAPD fragments","volume":"10","author":"Liu","year":"2020","journal-title":"3 Biotech"},{"key":"10.1016\/j.fochms.2025.100252_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxicon.2023.107554","article-title":"Chlorophyllum molybdites poisoning on Reunion Island","volume":"237","author":"Maillot","year":"2024","journal-title":"Toxicon"},{"key":"10.1016\/j.fochms.2025.100252_bb0125","doi-asserted-by":"crossref","first-page":"75","DOI":"10.35535\/pfsyst-2022-0009","article-title":"Introducing a new member of the genus Chlorophyllum: Chlorophyllum sapukai sp. nov., and new records from Paraguay","volume":"67","author":"Maubet","year":"2022","journal-title":"Plant and Fungal Systematics"},{"issue":"3","key":"10.1016\/j.fochms.2025.100252_bb0130","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1590\/S1516-89132007000300014","article-title":"Incidences of poisonings due to Chlorophyllum molybdites in the state of Paran\u00e1, Brazil","volume":"50","author":"Meijer","year":"2007","journal-title":"Brazilian Archives of Biology and Technology"},{"issue":"3","key":"10.1016\/j.fochms.2025.100252_bb0135","first-page":"213","article-title":"Case series of Chlorophyllum molybdites intoxication in Sicily: An \u201calien\u201d mushroom species in Europe","volume":"58","author":"Negrini","year":"2022","journal-title":"Annali dell\u2019Istituto Superiore di Sanit\u00e0"},{"key":"10.1016\/j.fochms.2025.100252_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2021.108182","article-title":"Foodborne illness caused by muscarine containing mushrooms and identification of mushroom remnants using phylogenetics and LC-MS\/MS","volume":"128","author":"Parnmen","year":"2021","journal-title":"Food Control"},{"issue":"3","key":"10.1016\/j.fochms.2025.100252_bb0145","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s43188-019-00024-2","article-title":"Phylogenetic evidence revealed Cantharocybe virosa (Agaricales, Hygrophoraceae) as a new clinical record for gastrointestinal mushroom poisoning in Thailand","volume":"36","author":"Parnmen","year":"2020","journal-title":"Toxicological Research"},{"key":"10.1016\/j.fochms.2025.100252_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2023.110239","article-title":"Discrimination of lethal Russula subnigricans from wild edible and morphologically similar mushrooms in the genus Russula using SCAR markers","volume":"158","author":"Parnmen","year":"2024","journal-title":"Food Control"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0155","first-page":"1","article-title":"Proximate analysis and antimicrobial activity of cultivated Macrolepiota dolichaula strain MFLUCC-13-0579","volume":"21","author":"Rizal","year":"2016","journal-title":"Asia-Pacific Journal of Science and Technology"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0160","first-page":"12","article-title":"Additions to the knowledge of Macrolepiota (Agaricales, Basidiomycota) in Northern Thailand","volume":"4","author":"Rizal","year":"2017","journal-title":"Bhutan Journal of Natural Resources & Development"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0165","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13002-022-00521-z","article-title":"Ethnomycological study of wild edible and medicinal mushrooms in district Jammu, J&K (UT), India","volume":"18","author":"Sharma","year":"2022","journal-title":"Journal of Ethnobiology and Ethnomedicine"},{"issue":"5","key":"10.1016\/j.fochms.2025.100252_bb0170","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1111\/j.2041-210X.2012.00215.x","article-title":"Advances in multiplex PCR: Balancing primer efficiencies and improving detection success","volume":"3","author":"Sint","year":"2012","journal-title":"Methods in Ecology and Evolution"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0175","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3109\/15563659008993488","article-title":"Chlorophyllum molybdites--Severe mushroom poisoning in a child","volume":"28","author":"Stenklyft","year":"1990","journal-title":"Journal of Toxicology. Clinical Toxicology"},{"key":"10.1016\/j.fochms.2025.100252_bb0180","article-title":"Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus","volume":"5","author":"Sugano","year":"2022","journal-title":"Food Chemistry: Molecular Sciences"},{"issue":"12","key":"10.1016\/j.fochms.2025.100252_bb0185","doi-asserted-by":"crossref","first-page":"666","DOI":"10.3390\/d13120666","article-title":"Description of Lepiotaceous fungal species of the genera Chlorophyllum, Clarkeinda, Macrolepiota, Pseudolepiota, and Xanthagaricus, from Laos and Thailand","volume":"13","author":"Sysouphanthong","year":"2021","journal-title":"Diversity"},{"issue":"7","key":"10.1016\/j.fochms.2025.100252_bb0190","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Molecular Biology and Evolution"},{"issue":"4","key":"10.1016\/j.fochms.2025.100252_bb0195","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3390\/jof10040269","article-title":"Development of SCAR markers for genetic authentication of Metarhizium acridum","volume":"10","author":"Toriello","year":"2024","journal-title":"Journal of fungi (Basel)"},{"key":"10.1016\/j.fochms.2025.100252_bb0200","series-title":"Flora Agaricina Neerlandica","first-page":"6","article-title":"Glossary","volume":"5","author":"Vellinga","year":"2001"},{"issue":"8","key":"10.1016\/j.fochms.2025.100252_bb0205","doi-asserted-by":"crossref","first-page":"4238","DOI":"10.1128\/jb.172.8.4238-4246.1990","article-title":"Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species","volume":"172","author":"Vilgalys","year":"1990","journal-title":"Journal of Bacteriology"},{"key":"10.1016\/j.fochms.2025.100252_bb0210","article-title":"Rapid and visual identification of Chlorophyllum molybdites with loop-mediated isothermal amplification method","volume":"12","author":"Wang","year":"2021","journal-title":"Frontiers in Microbiology"},{"issue":"1","key":"10.1016\/j.fochms.2025.100252_bb0215","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s13002-022-00551-7","article-title":"Ethnomycological study on wild mushrooms in Pu\u2019er Prefecture, Southwest Yunnan, China","volume":"18","author":"Wang","year":"2022","journal-title":"Journal of Ethnobiology and Ethnomedicine"},{"key":"10.1016\/j.fochms.2025.100252_bb0220","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.toxicon.2018.11.007","article-title":"Mushroom poisoning: A proposed new clinical classification","volume":"157","author":"White","year":"2019","journal-title":"Toxicon"},{"key":"10.1016\/j.fochms.2025.100252_bb0225","series-title":"PCR protocols: A guide to methods and applications","first-page":"315","article-title":"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics","author":"White","year":"1990"},{"issue":"3","key":"10.1016\/j.fochms.2025.100252_bb0230","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/jam.13647","article-title":"Loop-mediated isothermal amplification (LAMP): A versatile technique for detection of micro-organisms","volume":"124","author":"Wong","year":"2018","journal-title":"Journal of Applied Microbiology"},{"issue":"2","key":"10.1016\/j.fochms.2025.100252_bb0235","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.exppara.2012.07.007","article-title":"Opisthorchis viverrini and Haplorchis taichui: Development of a multiplex PCR assay for their detection and differentiation using specific primers derived from HAT-RAPD","volume":"132","author":"Wongsawad","year":"2012","journal-title":"Experimental Parasitology"},{"issue":"22","key":"10.1016\/j.fochms.2025.100252_bb0240","doi-asserted-by":"crossref","first-page":"6583","DOI":"10.1016\/j.bmc.2012.09.036","article-title":"Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool","volume":"20","author":"Yamada","year":"2012","journal-title":"Chlorophyllum molybdites. Bioorganic & Medicinal Chemistry"},{"key":"10.1016\/j.fochms.2025.100252_bb0245","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.669458","article-title":"Development, identification, and application of a germplasm specific SCAR marker for Dendrobium officinale Kimura et Migo","volume":"12","author":"Zheng","year":"2021","journal-title":"Frontiers in Plant Science"}],"container-title":["Food Chemistry: Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666566225000139?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666566225000139?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T13:27:14Z","timestamp":1750685234000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666566225000139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":49,"alternative-id":["S2666566225000139"],"URL":"https:\/\/doi.org\/10.1016\/j.fochms.2025.100252","relation":{},"ISSN":["2666-5662"],"issn-type":[{"type":"print","value":"2666-5662"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Novel SCAR markers for accurate diagnosis of toxic Chlorophyllum molybdites and C. globosum","name":"articletitle","label":"Article Title"},{"value":"Food Chemistry: Molecular Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fochms.2025.100252","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100252"}}