{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:07:35Z","timestamp":1792613255098,"version":"4.5.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2006,4]]},"DOI":"10.1016\/j.fsi.2005.06.008","type":"journal-article","created":{"date-parts":[[2005,8,16]],"date-time":"2005-08-16T11:12:58Z","timestamp":1124190778000},"page":"493-502","source":"Crossref","is-referenced-by-count":25,"title":["A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes"],"prefix":"10.1016","volume":"20","author":[{"given":"E.","family":"Aton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Renault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Gagnaire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Thomas-Guyon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cognard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Imbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2005.06.008_bib1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1051\/alr:1996015","article-title":"Cytotoxic and antibacterial properties of Mytilus Galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph","volume":"9","author":"Hubert","year":"1996","journal-title":"Aquatic Living Resources"},{"key":"10.1016\/j.fsi.2005.06.008_bib2","unstructured":"Oubella R. Reponse Immunitaire chez les mollusques bivalves. Effets de facteurs environnementaux sur le syst\u00e8me de d\u00e9fense interne. Doctorat; 1996."},{"key":"10.1016\/j.fsi.2005.06.008_bib3","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/361315a0","article-title":"Inositol triphosphate and calcium signalling","volume":"361","author":"Berridge","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2005.06.008_bib4","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1152\/physrev.1994.74.3.595","article-title":"Molecular and cellular physiology of intracellular calcium stores","volume":"74","author":"Pozzan","year":"1994","journal-title":"Physiological Reviews"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1054\/ceca.2000.0131","article-title":"Abnormal intracellular Ca2+ homeostasis and disease","volume":"28","author":"Missiaen","year":"2000","journal-title":"Cell Calcium"},{"key":"10.1016\/j.fsi.2005.06.008_bib6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S1095-6433(99)00118-X","article-title":"Voltage-activated currents in cardiac myocytes of the blue mussel, Mytilus edulis","volume":"124","author":"Curtis","year":"1999","journal-title":"Comparative Biochemistry and Physiology Part A"},{"key":"10.1016\/j.fsi.2005.06.008_bib7","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1006\/bbrc.1999.0405","article-title":"Cyclic ADP-ribose-dependant Ca2+ release is modulated by free [Ca2+] in the scallop sarcoplasmic reticulum","volume":"257","author":"Panfoli","year":"1999","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"10.1016\/j.fsi.2005.06.008_bib8","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0248-4900(00)01069-8","article-title":"Role of dihydropyridine-sensitive calcium channels in meiosis and fertilization in the bivalve molluscs Ruditapes philippinarum and Crassostrea gigas","volume":"92","author":"Leclerc","year":"2000","journal-title":"Biology of the Cell"},{"key":"10.1016\/j.fsi.2005.06.008_bib9","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1091\/mbc.10.2.393","article-title":"The Small Mr ras-like GTPase Rap1 and the phospholipase C pathway act to regulate phagocytosis in Dictyostelium discoideum","volume":"10","author":"Seastone","year":"1999","journal-title":"Molecular Biology of the Cell"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib10","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0300-9629(96)00400-8","article-title":"Calcium homeostasis and yeast phagocytosis in hemocytes of the colonial ascidian Botryllus schlossery","volume":"118","author":"Ballarin","year":"1997","journal-title":"Comparative Biochemistry and Physiology Part A: Physiology"},{"issue":"4","key":"10.1016\/j.fsi.2005.06.008_bib11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1054\/ceca.2000.0184","article-title":"Calcium requirement for efficient phagocytosis by Dictostelium discoideum","volume":"29","author":"Yuan","year":"2001","journal-title":"Cell Calcium"},{"key":"10.1016\/j.fsi.2005.06.008_bib12","doi-asserted-by":"crossref","first-page":"C1461","DOI":"10.1152\/ajpcell.00429.2001","article-title":"Essential role of Ca2+-dependent phospholipase A2 in estradiol-induced lysosome activation","volume":"283","author":"Burlando","year":"2002","journal-title":"American Journal of Physiology \u2013 Cell Physiology"},{"issue":"19","key":"10.1016\/j.fsi.2005.06.008_bib13","doi-asserted-by":"crossref","first-page":"3557","DOI":"10.1242\/jcs.114.19.3557","article-title":"Noradrenaline and alpha-adrenergic signaling induce the hsp70 gene promoter in mollusc immune cells","volume":"114","author":"Lacoste","year":"2001","journal-title":"Journal of Cell Sciences"},{"key":"10.1016\/j.fsi.2005.06.008_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0145-305X(01)00067-2","article-title":"Stress-induced immune changes in the oyster Crassostrea gigas","volume":"26","author":"Lacoste","year":"2001","journal-title":"Developmental and Comparative Immunology"},{"key":"10.1016\/j.fsi.2005.06.008_bib15","first-page":"295","article-title":"Platelet-derived growth factor and transforming growth factor-B in invertebrate immune and neuroendocrine interactions: another sign of conservation in evolution","volume":"129","author":"Ottaviani","year":"2001","journal-title":"Comparative Biochemistry and Physiology Part C"},{"issue":"2","key":"10.1016\/j.fsi.2005.06.008_bib16","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.aquatox.2003.09.003","article-title":"Mercury- and copper-induced lysosomal membrane destabilisation depends on [Ca2+]i dependent phospholipase A2 activation","volume":"66","author":"Marchi","year":"2004","journal-title":"Aquatic Toxicology"},{"key":"10.1016\/j.fsi.2005.06.008_bib17","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/BF00354318","article-title":"Effects of Hg2+ and Cu2+ on the cytosolic Ca2+ level in molluscan blood cells evaluated by confocal microscopy and spectrofluorimetry","volume":"119","author":"Viarengo","year":"1994","journal-title":"Marine Biology"},{"key":"10.1016\/j.fsi.2005.06.008_bib18","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2174\/1381612033392305","article-title":"Study of atrazine effects on pacific oyster, Crassostrea gigas, haemocytes","volume":"9","author":"Gagnaire","year":"2003","journal-title":"Current Pharmaceutical Design"},{"key":"10.1016\/j.fsi.2005.06.008_bib19","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1242\/jeb.200.15.2123","article-title":"Evidence for cholinergic inhibitory and serotonergic excitatory neuromuscular transmission in the heart of the bivalve Mercenaria mercenaria","volume":"200","author":"Kuwasawa","year":"1997","journal-title":"Journal of Experimental Biology"},{"key":"10.1016\/j.fsi.2005.06.008_bib20","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1006\/fsim.2001.0335","article-title":"Flow cytometric assessment of haemocyte sub-population in the European flat oyster, Ostrea edulis, haemolymph","volume":"11","author":"Xue","year":"2001","journal-title":"Fish and Shellfish Immunology"},{"key":"10.1016\/j.fsi.2005.06.008_bib21","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0091-679X(08)61114-0","article-title":"Practical aspects of measuring [Ca2+] with fluorescent indicators","volume":"40","author":"Kao","year":"1994","journal-title":"Methods in Cell Biology"},{"issue":"14","key":"10.1016\/j.fsi.2005.06.008_bib22","doi-asserted-by":"crossref","first-page":"6300","DOI":"10.1016\/S0021-9258(19)84563-5","article-title":"Ryanodine activation and inhibition of the Ca2+ release channel of sarcoplasmic reticulum","volume":"261","author":"Meissner","year":"1986","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0145-305X(97)00004-9","article-title":"Activation of prophenoloxidase in the plasma and haemocytes of the marine mussel Perna viridis","volume":"21","author":"Asokan","year":"1997","journal-title":"Developmental and Comparative Immunology"},{"key":"10.1016\/j.fsi.2005.06.008_bib24","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S1095-6433(01)00447-0","article-title":"Organic and inorganic compound variations in haemolymph, epidermal tissue and cuticle over the molt cycle in Scylla serrata (Decapoda)","volume":"131","author":"Pratoomchat","year":"2002","journal-title":"Comparative Biochemistry and Physiology Part A"},{"issue":"1\u20132","key":"10.1016\/j.fsi.2005.06.008_bib25","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0044-8486(98)00439-6","article-title":"Defense mechanisms and diseases prevention in farmed marine invertebrates","volume":"172","author":"Roch","year":"1999","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2005.06.008_bib26","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S1050-4648(02)00164-X","article-title":"Structural and functional characterization of the blood cells of the bivalve mollusc, Scrobicularia plana","volume":"15","author":"Wootton","year":"2003","journal-title":"Fish and Shellfish Immunology"},{"key":"10.1016\/j.fsi.2005.06.008_bib27","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0006-8993(99)01105-1","article-title":"Ryanodine receptor modulation of in vitro associative learning in Hermissenda crassicornis","volume":"822","author":"Blackwell","year":"1999","journal-title":"Brain Research"},{"key":"10.1016\/j.fsi.2005.06.008_bib28","doi-asserted-by":"crossref","first-page":"7157","DOI":"10.1128\/jb.172.12.7157-7166.1990","article-title":"Role of Escherichia coli heat shock proteins DnaK and HtpG (C62.5) in response to nutritional deprivation","volume":"172","author":"Spence","year":"1990","journal-title":"Journal of Bacteriology"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib29","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/(SICI)1097-4644(20000401)77:1<127::AID-JCB13>3.0.CO;2-0","article-title":"Regulation of heat shock protein message in Jurkat cells cultured under serum-starved and gravity-altered conditions","volume":"77","author":"Lewis","year":"2000","journal-title":"Journal of Cell Biochemistry"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib30","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3109\/10623329909165310","article-title":"Apoptosis in vascular endothelial cells caused by serum deprivation, oxidative stress and transforming growth factor-beta","volume":"7","author":"Hogg","year":"1999","journal-title":"Endothelium"},{"key":"10.1016\/j.fsi.2005.06.008_bib31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0091-679X(08)61569-1","article-title":"Making informed choices: medium, serum, and serum-free medium. How to choose the appropriate medium and culture system for the model you wish to create","volume":"57","author":"Mather","year":"1998","journal-title":"Methods of Cell Biology"},{"issue":"3","key":"10.1016\/j.fsi.2005.06.008_bib32","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1159\/000016312","article-title":"Impact of culture conditions, culture media volumes, and glucose content on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic?","volume":"9","author":"Gstraunthaler","year":"1999","journal-title":"Cell Physiology and Biochemistry"},{"issue":"2","key":"10.1016\/j.fsi.2005.06.008_bib33","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1054\/ceca.1999.0095","article-title":"Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes","volume":"27","author":"Gee","year":"2000","journal-title":"Cell Calcium"},{"key":"10.1016\/j.fsi.2005.06.008_bib34","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved florescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"10.1016\/j.fsi.2005.06.008_bib35","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0006-291X(91)91966-G","article-title":"The effect of pH on rate constants, ion selectivity and thermodynamic properties of fluorescent calcium and magnesium indicators","volume":"177","author":"Lattanzio","year":"1991","journal-title":"Biochemistry and Biophysical Research Communications"},{"issue":"9","key":"10.1016\/j.fsi.2005.06.008_bib36","doi-asserted-by":"crossref","first-page":"4887","DOI":"10.4049\/jimmunol.167.9.4887","article-title":"Expression of the ryanodine receptor isoforms in immune cells","volume":"167","author":"Hosoi","year":"2001","journal-title":"Journal of Immunology"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464805001257?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464805001257?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T21:17:57Z","timestamp":1626383877000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464805001257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["S1050464805001257"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2005.06.008","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2006,4]]}}}