{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T15:50:28Z","timestamp":1778773828503,"version":"3.51.4"},"reference-count":42,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2008,2]]},"DOI":"10.1016\/j.fsi.2007.10.016","type":"journal-article","created":{"date-parts":[[2007,11,26]],"date-time":"2007-11-26T15:24:10Z","timestamp":1196090650000},"page":"215-222","source":"Crossref","is-referenced-by-count":26,"title":["Complement-mediated killing of Vibrio species by the humoral fluids of amphioxus Branchiostoma belcheri: Implications for a dual role of O-antigens in the resistance to bactericidal activity"],"prefix":"10.1016","volume":"24","author":[{"given":"Zhimin","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shicui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changfa","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuxiang","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2007.10.016_bib1","first-page":"561","article-title":"Ueber die nahere Natur der bekterientodtenden Substanzim Blutserum","volume":"6","author":"Bucherner","year":"1889","journal-title":"Central Bakteriol Parasitol"},{"key":"10.1016\/j.fsi.2007.10.016_bib2","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/BF02188097","article-title":"Experimente uber die bakterienfeidlichen Einflusse des thierischen Korpers","volume":"4","author":"Nuttal","year":"1888","journal-title":"Z Hyg Infektionskr"},{"key":"10.1016\/j.fsi.2007.10.016_bib3","doi-asserted-by":"crossref","first-page":"R595","DOI":"10.1016\/S0960-9822(98)70384-7","article-title":"Innate immunity","volume":"8","author":"Robertson","year":"1998","journal-title":"Curr Biol"},{"key":"10.1016\/j.fsi.2007.10.016_bib4","first-page":"1","article-title":"The immunobiology of complement. Chemistry and biologic activity of the complement system","volume":"6","author":"Ruddy","year":"1974","journal-title":"Transplant Proc"},{"key":"10.1016\/j.fsi.2007.10.016_bib5","series-title":"The fish immune system: organism, pathogen and environment","first-page":"105","article-title":"The nonspecific immune system: humoral defence","author":"Yano","year":"1996"},{"key":"10.1016\/j.fsi.2007.10.016_bib6","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.2108\/zsj.20.1207","article-title":"Presence and characterization of complement-like activity in the amphioxus Branchiostoma belcheri tsingtauense","volume":"20","author":"Zhang","year":"2003","journal-title":"Zool Sci"},{"key":"10.1016\/j.fsi.2007.10.016_bib7","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1645\/0022-3395(2000)086[1223:ACSOMA]2.0.CO;2","article-title":"A comparative study of mammalian and reptilian alternative pathway of complement-mediated killing of Lyme disease spirochete (Borrelia burgdoferi)","volume":"86","author":"Kuo","year":"2000","journal-title":"J Parasitol"},{"key":"10.1016\/j.fsi.2007.10.016_bib8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3109\/10408369509084686","article-title":"Physiology and pathophysiology of complement: progress and trends","volume":"32","author":"Morgan","year":"1995","journal-title":"Crit Rev Clin Lab Sci"},{"key":"10.1016\/j.fsi.2007.10.016_bib9","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/386506a0","article-title":"A second serine protease associated with mannan-binding lectin that activates complement","volume":"386","author":"Thiel","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2007.10.016_bib10","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/0167-5699(96)10062-1","article-title":"Mannose-binding lectin: the pluripotent molecule of the innate immune system","volume":"17","author":"Turner","year":"1996","journal-title":"Immunol Today"},{"key":"10.1016\/j.fsi.2007.10.016_bib11","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1645\/0022-3395(2000)086[1232:CMKOBB]2.0.CO;2","article-title":"Complement-mediated killing of Borrelia burgdorferi by nonimmune sera from sika deer","volume":"86","author":"Nelson","year":"2000","journal-title":"J Parasitol"},{"key":"10.1016\/j.fsi.2007.10.016_bib12","doi-asserted-by":"crossref","first-page":"6577","DOI":"10.1128\/IAI.73.10.6577-6583.2005","article-title":"Reduction in capsular content and enhanced bacterial susceptibility to serum killing of Vibrio cholerae O139 associated with the 2002 cholera epidemic in Bangladesh","volume":"73","author":"Qadri","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2007.10.016_bib13","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1645\/Ge-3081RN","article-title":"Bacteriolytic activity of selected vertebrate sera for Borrelia burgdorferi sensu stricto and Borrelia bissettii","volume":"89","author":"Ullmann","year":"2003","journal-title":"J Parasitol"},{"key":"10.1016\/j.fsi.2007.10.016_bib14","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/AEM.64.2.515-519.1998","article-title":"Determination of complement-mediated killing of bacteria by viability staining and bioluminescence","volume":"64","author":"Virta","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.fsi.2007.10.016_bib15","doi-asserted-by":"crossref","first-page":"4687","DOI":"10.1074\/jbc.275.7.4687","article-title":"Molecular aspects of complement-mediated bacterial killing: periplasmic conversion of C9 from a protoxin to a toxin","volume":"275","author":"Wang","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2007.10.016_bib16","first-page":"1285","article-title":"Interaction of turkey complement with Escherichia coli isolated from turkeys","volume":"50","author":"Ellis","year":"1989","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.fsi.2007.10.016_bib17","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0145-305X(01)00038-6","article-title":"Innate host defense mechanisms of fish against viruses and bacteria","volume":"25","author":"Ellis","year":"2001","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib18","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0165-2427(94)05430-Z","article-title":"Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are affected by the alternative complement pathway","volume":"45","author":"Sunyer","year":"1995","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.fsi.2007.10.016_bib19","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.fsi.2005.08.011","article-title":"Complement consumption by Photobacterium damselae subsp. Piscicida in seabream, red porgy and seabass normal and immune serum. Effect of the capsule on the bactericidal effect","volume":"20","author":"Acosta","year":"2006","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib20","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.fsi.2005.04.004","article-title":"Recent advances on the complement system in teleost fish","volume":"20","author":"Boshra","year":"2006","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib21","series-title":"Virulence mechanisms of bacterial pathogens","first-page":"107","article-title":"Bacterial resistance to complement","author":"Taylor","year":"1988"},{"key":"10.1016\/j.fsi.2007.10.016_bib22","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1126\/science.284.5418.1313","article-title":"Phylogenetic perspectives in innate immunity","volume":"284","author":"Hoffmann","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.fsi.2007.10.016_bib23","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/35066006","article-title":"The evolution and genetics of innate immunity","volume":"2","author":"Kimbrell","year":"2001","journal-title":"Nat Rev Genet"},{"key":"10.1016\/j.fsi.2007.10.016_bib24","doi-asserted-by":"crossref","first-page":"2983","DOI":"10.4049\/jimmunol.160.6.2983","article-title":"Sea urchin coelomocytes specifically express a homologue of the complement component C3","volume":"160","author":"Al-Sharif","year":"1998","journal-title":"J Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib25","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s00251-006-0142-1","article-title":"Genomic view of the evolution of the complement system","volume":"58","author":"Nonaka","year":"2006","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2007.10.016_bib26","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0162-3109(99)00009-0","article-title":"Complement systems in invertebrates. The ancient alternative and lectin pathways","volume":"42","author":"Smith","year":"1999","journal-title":"Immunopharmacology"},{"key":"10.1016\/j.fsi.2007.10.016_bib27","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s00239-001-0068-z","article-title":"C6-like and C3-like molecules from the cephalochordate, amphioxus, suggest a cytolytic complement system in invertebrates","volume":"54","author":"Suzuki","year":"2002","journal-title":"J Mol Evol"},{"key":"10.1016\/j.fsi.2007.10.016_bib28","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/sj.emboj.7600533","article-title":"The ancient origin of the complement system","volume":"24","author":"Zhu","year":"2005","journal-title":"EMBO J"},{"key":"10.1016\/j.fsi.2007.10.016_bib29","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s002849900041","article-title":"Virulence of Vibrio alginolyticusisolated from diseased tiger prawn Penaeus monodon","volume":"32","author":"Lee","year":"1996","journal-title":"Curr Microbiol"},{"key":"10.1016\/j.fsi.2007.10.016_bib30","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1163\/156856001760209789","article-title":"Presence and induction by bacteria of d-galactoside-specific lectins in the humoral fluids of amphioxus Branchiostoma belcheri tsingtauense","volume":"9","author":"Wang","year":"2002","journal-title":"Inflammopharmacology"},{"key":"10.1016\/j.fsi.2007.10.016_bib31","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.fsi.2007.10.016_bib32","doi-asserted-by":"crossref","first-page":"387","DOI":"10.4049\/jimmunol.162.1.387","article-title":"Opsonic complement component C3 in the solitary ascidian, Halocynthia roretzi","volume":"162","author":"Nonaka","year":"1999","journal-title":"J Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib33","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.fsi.2003.11.005","article-title":"Evidence of complement-mediated killing of Discocotyle sagittata (Platyhelminthes, Monogenea) oncomiracidia","volume":"17","author":"Rubio-Godoy","year":"2004","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib34","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/JB.154.1.269-277.1983","article-title":"Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels","volume":"154","author":"Hitchcock","year":"1983","journal-title":"J Bacteriol"},{"key":"10.1016\/j.fsi.2007.10.016_bib35","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2007.10.016_bib36","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1128\/JCM.28.12.2627-2631.1990","article-title":"Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels","volume":"28","author":"Fomsgaard","year":"1990","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.fsi.2007.10.016_bib37","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1128\/IAI.67.1.294-301.1999","article-title":"Vibrio anguillarum resistance to rainbow trout (Oncorhynchus mykiss) serum: role of O-antigen structure of lipopolysaccharide","volume":"67","author":"Boesen","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2007.10.016_bib38","doi-asserted-by":"crossref","first-page":"696","DOI":"10.4049\/jimmunol.114.2_Part_1.696","article-title":"Alternate complement pathway induction of aggregation and release of 5-hydroxytryptamine and adenosine diphosphate by rabbit platelets","volume":"114","author":"Marney","year":"1975","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2007.10.016_bib39","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1432-1033.1980.tb04635.x","article-title":"Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli O111 and Salmonella typhimurium LT2","volume":"107","author":"Goldman","year":"1980","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.fsi.2007.10.016_bib40","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1128\/IAI.36.3.1069-1075.1982","article-title":"Role of surface components in serum resistance of virulent Aeromonas salmonicida","volume":"36","author":"Munn","year":"1982","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2007.10.016_bib41","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3181\/00379727-133-34472","article-title":"The sensitivity of smooth and rough Gram-negative bacteria to the immune bactericidal reaction","volume":"133","author":"Muschel","year":"1970","journal-title":"Proc Soc Exp Biol Med"},{"key":"10.1016\/j.fsi.2007.10.016_bib42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1128\/IAI.54.1.85-89.1986","article-title":"Role of capsule and O antigen in resistance of Klebsiella pneumomae to serum bactericidal activity","volume":"54","author":"Tom\u00e1s","year":"1986","journal-title":"Infect Immun"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464807001908?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464807001908?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,14]],"date-time":"2023-05-14T22:58:18Z","timestamp":1684105098000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464807001908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["S1050464807001908"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2007.10.016","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2008,2]]}}}