{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:58:18Z","timestamp":1790913498025,"version":"4.1.0"},"reference-count":57,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2010,4]]},"DOI":"10.1016\/j.fsi.2009.12.027","type":"journal-article","created":{"date-parts":[[2010,1,8]],"date-time":"2010-01-08T10:36:45Z","timestamp":1262947005000},"page":"661-671","source":"Crossref","is-referenced-by-count":18,"title":["Disk abalone (Haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: Molecular cloning and immune response against bacterial infection"],"prefix":"10.1016","volume":"28","author":[{"given":"Chamilani","family":"Nikapitiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahanama","family":"De Zoysa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulhong","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngdeuk","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashani Mudika","family":"Ekanayake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilson","family":"Whang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheol Young","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Seong","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jehee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2009.12.027_bib1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.bi.49.070180.003113","article-title":"Protein inhibitors of proteinases","volume":"49","author":"Laskowski","year":"1980","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.fsi.2009.12.027_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0049-3848(94)90204-6","article-title":"The coagulation cascade: initiation, maintenance, and regulation","volume":"76","author":"Takada","year":"1994","journal-title":"Thromb Res"},{"key":"10.1016\/j.fsi.2009.12.027_bib3","doi-asserted-by":"crossref","first-page":"6819","DOI":"10.1073\/pnas.95.12.6819","article-title":"The gastrulation defective gene of Drosophila melanogaster is a member of the serine protease superfamily","volume":"95","author":"Konrad","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.fsi.2009.12.027_bib4","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1042\/bj20031825","article-title":"Evolutional families of peptidase inhibitors","volume":"378","author":"Rawlings","year":"2004","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2009.12.027_bib5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0378-1119(00)00181-5","article-title":"Sp22D: a multidomain serine protease with a putative role in insect immunity","volume":"251","author":"Gorman","year":"2000","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2009.12.027_bib6","series-title":"Old Herborn University seminar monograph II. Immunomodulation of the gastrointestinal mucosa","first-page":"59","article-title":"Mechanisms of the intestinal immunity to nematode parasites and the consequences to invasion by opportunistic bacteria","author":"Urban","year":"1998"},{"key":"10.1016\/j.fsi.2009.12.027_bib7","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1046\/j.1440-1711.1999.00834.x","article-title":"Modulation of immune function by a modified bovine whey protein concentrate","volume":"774","author":"Cross","year":"1999","journal-title":"Immunol Cell Biol"},{"key":"10.1016\/j.fsi.2009.12.027_bib8","doi-asserted-by":"crossref","first-page":"9718","DOI":"10.1016\/S0021-9258(18)47993-8","article-title":"Isolation and characterization of antistasin, an inhibitor of metastasis and coagulation","volume":"262","author":"Tuszinsky","year":"1987","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib9","first-page":"1633","article-title":"Inhibition of lung tumor colonization by leech salivary gland extracts from Haementeria ghilianii","volume":"43","author":"Gasic","year":"1983","journal-title":"Cancer Res"},{"key":"10.1016\/j.fsi.2009.12.027_bib10","first-page":"5670","article-title":"Leech salivary gland extract from Haementeria officinalis, potent inhibitor of cyclophosphamide and radiation induced artificial metastasis enhancement","volume":"44","author":"Gasic","year":"1984","journal-title":"Cancer Res"},{"key":"10.1016\/j.fsi.2009.12.027_bib11","first-page":"44","article-title":"Hementin: anti-coagulant protease from the salivary gland of the leech Haementeria ghilianii","volume":"103","author":"Malinconico","year":"1984","journal-title":"J Lab Clin Med"},{"key":"10.1016\/j.fsi.2009.12.027_bib12","doi-asserted-by":"crossref","first-page":"6369","DOI":"10.1093\/nar\/12.16.6369","article-title":"A large fragment approach to DNA synthesis: total synthesis of a gene for the protease inhibitor eglin c from the leech Hirudo medicinalis and its expression in E. coli","volume":"12","author":"Rink","year":"1984","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.fsi.2009.12.027_bib13","first-page":"1155","article-title":"Eglinc, a polypeptide derived from the medicinal leech, prevents human neutrophil elastase-induced emphysema and bronchial secretory cell metaplasia in the hamster","volume":"132","author":"Snyder","year":"1985","journal-title":"Am Rev Respir Dis"},{"key":"10.1016\/j.fsi.2009.12.027_bib14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1055\/s-0038-1661011","article-title":"Protease inhibitors in Haementeria leech species","volume":"51","author":"Murer","year":"1984","journal-title":"Thromb Haemost"},{"issue":"1","key":"10.1016\/j.fsi.2009.12.027_bib15","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0378-1119(89)90382-X","article-title":"Cloning and expression of cDNA encoding antistasin, a leech-derived protein having anti-coagulant and anti-metastatic properties","volume":"75","author":"Han","year":"1989","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2009.12.027_bib16","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/0014-5793(92)80791-E","article-title":"The primitive metazoan Hydra expresses antistasin, a serine protease inhibitor of vertebrate blood coagulation: cDNA cloning, cellular localization and developmental regulation","volume":"309","author":"Holstein","year":"1992","journal-title":"FEBS Lett"},{"key":"10.1016\/j.fsi.2009.12.027_bib17","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/S0021-9258(17)42383-0","article-title":"A limulus intracellular coagulation inhibitor with characteristics of the serpin super family. Purification, characterization, and cDNA cloning","volume":"269","author":"Miura","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib18","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1074\/jbc.270.2.558","article-title":"A limulus intracellular coagulation inhibitor type 2. Purification, characterization, cDNA cloning, and tissue localization","volume":"270","author":"Miura","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib19","series-title":"New directions in invertebrate immunology","first-page":"155","article-title":"Proteinase inhibitors in invertebrate immunity","author":"Kanost","year":"1996"},{"key":"10.1016\/j.fsi.2009.12.027_bib20","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1074\/jbc.272.2.1082","article-title":"Characterization and functional analysis of 12 naturally occurring reactive site variants of serpin-1 from Manduca sexta","volume":"272","author":"Jiang","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib21","doi-asserted-by":"crossref","first-page":"6682","DOI":"10.1073\/pnas.94.13.6682","article-title":"Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain","volume":"94","author":"Liang","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.fsi.2009.12.027_bib22","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1126\/science.285.5435.1917","article-title":"Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila","volume":"285","author":"Levashina","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.fsi.2009.12.027_bib23","doi-asserted-by":"crossref","first-page":"6330","DOI":"10.1093\/emboj\/cdf661","article-title":"A serpin mutant links toll activation to melanization in the host defence of Drosophila","volume":"21","author":"Ligoxygakis","year":"2002","journal-title":"EMBO J"},{"key":"10.1016\/j.fsi.2009.12.027_bib24","doi-asserted-by":"crossref","first-page":"14923","DOI":"10.1074\/jbc.M500531200","article-title":"Manduca sexta serpin-4 and serpin-5 inhibit the prophenoloxidase activation pathway: cDNA cloning, protein expression, and characterization","volume":"280","author":"Tong","year":"2005","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib25","first-page":"559","article-title":"Properties of fungal protease inhibitors from the integument and haemolymph of the silkworm and effect of an inhibitor on the fungal growth","volume":"95B","author":"Yoshida","year":"1990","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib26","first-page":"537","article-title":"Purification and characterization of a fungal protease specific protein inhibitor (FPI-F) in the silkworm haemolymph","volume":"104B","author":"Eguchi","year":"1993","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib27","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1046\/j.1432-1327.2000.01207.x","article-title":"Isolation and characterization of novel inducible serine protease inhibitors from larval hemolymph of the greater wax moth Galleria mellonella","volume":"267","author":"Frobius","year":"2000","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.fsi.2009.12.027_bib28","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.cbpb.2006.05.010","article-title":"A novel slow-tight binding serine protease inhibitor from eastern oyster (Crassostrea virginica) plasma inhibits perkinsin, the major extracellular protease of the oyster protozoan parasite Perkinsus marinus","volume":"145B","author":"Xue","year":"2006","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib29","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.fsi.2009.05.006","article-title":"Evidence indicating the existence of a novel family of serine protease inhibitors that may be involved in marine invertebrate immunity","volume":"27","author":"Xue","year":"2009","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2009.12.027_bib30","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0305-0491(98)10084-6","article-title":"Evidence for the presence of protease inhibitors in Eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters","volume":"121B","author":"Faisal","year":"1998","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib31","first-page":"427","article-title":"Protease inhibitors in plasma of the softshell clam Mya arenaria: identification and effects of disseminated sarcoma","volume":"123B","author":"Ehab","year":"1999","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib32","first-page":"161","article-title":"Humoral immunity: \u03b12 macroglobulin activity in the plasma of mollusks","volume":"35","author":"Armstrong","year":"1992","journal-title":"Veliger"},{"key":"10.1016\/j.fsi.2009.12.027_bib33","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.fsi.2005.05.009","article-title":"Molecular cloning, characterization and expression of a novel serine proteinase inhibitor gene in bay scallops (Argopecten irradians, Lamarck 1819)","volume":"20","author":"Zhu","year":"2006","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2009.12.027_bib34","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1577\/1548-8667(1998)010<0271:EIOWHP>2.0.CO;2","article-title":"Experimental infections of western hemisphere Penaeid shrimp with Asian white spot syndrome virus and Asian yellow head virus","volume":"10","author":"Lightner","year":"1998","journal-title":"J Aquat Anim Health"},{"key":"10.1016\/j.fsi.2009.12.027_bib35","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0044-8486(03)00488-5","article-title":"Effect of dissolved oxygen on the immune response of Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus","volume":"232","author":"Cheng","year":"2004","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2009.12.027_bib36","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.fsi.2006.06.009","article-title":"Stress and immune responses in abalone: limitations in current knowledge and investigative methods based on other models","volume":"22","author":"Hooper","year":"2007","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2009.12.027_bib37","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.cbpb.2007.12.009","article-title":"Mitochondrial thioredoxin-2 from disk abalone (Haliotis discus discus): molecular characterization, tissue expression and DNA protection activity of its recombinant protein","volume":"149B","author":"De Zoysa","year":"2008","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib38","first-page":"12","article-title":"Basic alignment search tool","volume":"27","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"10.1016\/j.fsi.2009.12.027_bib39","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0076-6879(90)83007-V","article-title":"Rapid and sensitive sequence comparison with FASTP and FASTA","volume":"183","author":"Pearson","year":"1990","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.fsi.2009.12.027_bib40","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTALW: improving the sensitivity of\u00a0progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.fsi.2009.12.027_bib41","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1093\/bib\/5.2.150","article-title":"MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment","volume":"5","author":"Kumar","year":"2004","journal-title":"Brief Bioinform"},{"key":"10.1016\/j.fsi.2009.12.027_bib42","series-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.fsi.2009.12.027_bib43","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.fsi.2009.12.027_bib44","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real time quantitative PCR and the 2\u2206\u2206CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2009.12.027_bib45","doi-asserted-by":"crossref","first-page":"16694","DOI":"10.1016\/S0021-9258(19)84761-0","article-title":"Antistasin, a leech-derived inhibitor of factor Xa. Kinetic analysis of enzyme inhibition and identification of the reactive site","volume":"264","author":"Dunwiddie","year":"1989","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib46","first-page":"1","article-title":"A review of the most important classes of serine protease inhibitors in insects and leeches","volume":"2","author":"Clynen","year":"2005","journal-title":"Med Chem Rev"},{"key":"10.1016\/j.fsi.2009.12.027_bib47","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0969-2126(97)00183-4","article-title":"A new structural class of serine protease inhibitors revealed by the structure of the hirustasin\u2013kallekrein complex","volume":"5","author":"Mittl","year":"1997","journal-title":"Structure"},{"key":"10.1016\/j.fsi.2009.12.027_bib48","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/S0965-1748(01)00034-0","article-title":"A bacteria-induced, intracellular serpin in granular hemocytes of Manduca sexta","volume":"31","author":"Gan","year":"2001","journal-title":"Insect Biochem Mol Biol"},{"issue":"2\u20133","key":"10.1016\/j.fsi.2009.12.027_bib49","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/S0014-5793(00)01224-2","article-title":"A novel Drosophila serpin that inhibits serine proteases","volume":"468","author":"Han","year":"2000","journal-title":"FEBS Lett"},{"key":"10.1016\/j.fsi.2009.12.027_bib50","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.procbio.2007.11.011","article-title":"Purification and characterization of a novel anticoagulant peptide from marine echiuroid worm, Urechis unicinctus","volume":"43","author":"Jo","year":"2008","journal-title":"Process Biochem"},{"key":"10.1016\/j.fsi.2009.12.027_bib51","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0044-8486(97)00169-5","article-title":"Protein-hydrolyzing enzymes in the digestive systems of the adult Mexican blue abalone, Haliotis fulgens (Gastropoda)","volume":"157","author":"Serviere-Zaragoza","year":"1997","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2009.12.027_bib52","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S1096-4959(02)00221-X","article-title":"Digestive enzymes in juvenile green abalone, Haliotis fulgens, fed natural food","volume":"134B","author":"Garc\u0131a-Carreno","year":"2003","journal-title":"Comp Biochem Physiol"},{"key":"10.1016\/j.fsi.2009.12.027_bib53","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0952-7915(01)00302-8","article-title":"The molecular basis of innate immunity in the horseshoe crab","volume":"14","author":"Iwanaga","year":"2002","journal-title":"Curr Opin Immunol"},{"key":"10.1016\/j.fsi.2009.12.027_bib54","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1073\/pnas.0306904101","article-title":"A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides","volume":"101","author":"Ariki","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.fsi.2009.12.027_bib55","doi-asserted-by":"crossref","first-page":"15957","DOI":"10.1016\/S0021-9258(17)33954-6","article-title":"The serpin superfamily of proteinase inhibitors: structure, function, and regulation","volume":"269","author":"Potempa","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.fsi.2009.12.027_bib56","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/0965-1748(94)90141-4","article-title":"A target protease activity of serpins in insect hemolymph","volume":"24","author":"Ashida","year":"1994","journal-title":"Insect Biochem Mol Biol"},{"key":"10.1016\/j.fsi.2009.12.027_bib57","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1017\/S0953756297005509","article-title":"The inhibition of extracellular proteinases from Aphanomyces spp. by three different proteinase inhibitors from crayfish blood","volume":"102","author":"Dieguez-Uribeondo","year":"1998","journal-title":"Mycol Res"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464809004197?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464809004197?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T01:26:12Z","timestamp":1634693172000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464809004197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["S1050464809004197"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2009.12.027","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}