{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:21:55Z","timestamp":1790083315664,"version":"4.0.1"},"reference-count":47,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31025030"],"award-info":[{"award-number":["31025030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"863 High Technology Project of the Ministry of Science and Technology","award":["2011AA10A216"],"award-info":[{"award-number":["2011AA10A216"]}]},{"name":"863 High Technology Project of the Ministry of Science and Technology","award":["2012BAD17B01"],"award-info":[{"award-number":["2012BAD17B01"]}]},{"name":"Knowledge Innovation Program of the Chinese Academy of Sciences","award":["KZCX2-EW-Q213"],"award-info":[{"award-number":["KZCX2-EW-Q213"]}]},{"name":"Knowledge Innovation Program of the Chinese Academy of Sciences","award":["KSCX2-EW-G-12B"],"award-info":[{"award-number":["KSCX2-EW-G-12B"]}]},{"name":"Taishan Scholar Program of Shandong Province"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2013,2]]},"DOI":"10.1016\/j.fsi.2012.11.039","type":"journal-article","created":{"date-parts":[[2012,12,16]],"date-time":"2012-12-16T11:17:35Z","timestamp":1355656655000},"page":"514-520","source":"Crossref","is-referenced-by-count":23,"title":["Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity"],"prefix":"10.1016","volume":"34","author":[{"given":"Pan-pan","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-hua","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-guang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-gong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2012.11.039_bib1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.tim.2004.09.002","article-title":"Bacterial flagellins: mediators of pathogenicity and host immune responses in mucosa","volume":"12","author":"Ramos","year":"2004","journal-title":"Trends Microbiol"},{"key":"10.1016\/j.fsi.2012.11.039_bib2","doi-asserted-by":"crossref","first-page":"3597","DOI":"10.1128\/JB.183.12.3597-3605.2001","article-title":"Ralstonia solanacearum needs motility for invasive virulence on tomato","volume":"183","author":"Tans-Kersten","year":"2001","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.fsi.2012.11.039_bib3","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/0092-8674(95)90505-7","article-title":"The bacterial flagellum: from genetic net-work to complex architecture","volume":"80","author":"Shapiro","year":"1995","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2012.11.039_bib4","doi-asserted-by":"crossref","first-page":"15758","DOI":"10.1016\/S0021-9258(17)36323-8","article-title":"The covalent structure of the phase-1 flagellar filament protein of Salmonella typhimurium and its comparison with other flagellins","volume":"260","author":"Joys","year":"1985","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.fsi.2012.11.039_bib5","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1099\/00221287-143-10-3071","article-title":"The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis","volume":"143","author":"Winstanley","year":"1997","journal-title":"Microbiology"},{"key":"10.1016\/j.fsi.2012.11.039_bib6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/35066504","article-title":"Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling","volume":"410","author":"Samatey","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2012.11.039_bib7","doi-asserted-by":"crossref","first-page":"40456","DOI":"10.1074\/jbc.M206851200","article-title":"Two nonadjacent regions in enteroaggregative Escherichia coli flagellin are required for activation of toll like receptor 5","volume":"277","author":"Donnelly","year":"2002","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.fsi.2012.11.039_bib8","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1038\/ni1011","article-title":"Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility","volume":"4","author":"Smith","year":"2003","journal-title":"Nat Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib9","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.smim.2006.12.004","article-title":"TLR signaling","volume":"19","author":"Kawai","year":"2007","journal-title":"Semin Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib10","series-title":"Toll-like receptors (TLRs) and innate immunity","first-page":"240","article-title":"Toll-like receptors (TLRs) and their ligands","author":"Uematsu","year":"2008"},{"key":"10.1016\/j.fsi.2012.11.039_bib11","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2012.11.039_bib12","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.4049\/jimmunol.167.4.1882","article-title":"Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression","volume":"167","author":"Gewirtz","year":"2001","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib13","doi-asserted-by":"crossref","first-page":"5165","DOI":"10.4049\/jimmunol.170.10.5165","article-title":"The Toll-Like receptor 5 stimulus bacterial flagellin induces maturation and chemokine production in human dendritic cells","volume":"170","author":"Means","year":"2003","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib14","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2009.09.021","article-title":"Toll-like receptor signaling in bony fish","volume":"134","author":"Rebl","year":"2010","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.fsi.2012.11.039_bib15","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1128\/MMBR.65.3.445-462.2001","article-title":"Polar flagellar motility of the Vibrionaceae","volume":"65","author":"McCarter","year":"2001","journal-title":"Microbiol Mol Biol"},{"key":"10.1016\/j.fsi.2012.11.039_bib16","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1128\/jb.177.6.1595-1609.1995","article-title":"Genetic and molecular characterization of the polar flagellum of Vibrio parahaemolyticus","volume":"177","author":"McCarter","year":"1995","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.fsi.2012.11.039_bib17","doi-asserted-by":"crossref","first-page":"5188","DOI":"10.1128\/jb.178.17.5188-5198.1996","article-title":"Identification and characterization of additional flagellin genes from Vibrio anguillarum","volume":"178","author":"McGee","year":"1996","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.fsi.2012.11.039_bib18","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1128\/jb.178.5.1310-1319.1996","article-title":"Flagellin A is essential for the virulence of Vibrio anguillarum","volume":"178","author":"Milton","year":"1996","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.fsi.2012.11.039_bib19","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1128\/JB.182.8.2326-2328.2000","article-title":"Role of motility in adherence to and invasion of a fish cell line by Vibrio anguillarum","volume":"182","author":"Ormonde","year":"2000","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.fsi.2012.11.039_bib20","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1128\/IAI.59.8.2727-2736.1991","article-title":"Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: analysis of motility mutants in three animal models","volume":"59","author":"Richardson","year":"1991","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2012.11.039_bib21","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1128\/IAI.72.8.4905-4910.2004","article-title":"Role of flagellum and motility in pathogenesis of Vibrio vulnificus","volume":"72","author":"Lee","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2012.11.039_bib22","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.vaccine.2011.10.058","article-title":"Intranasal administration of a flagellin-adjuvanted inactivated influenza vaccine enhances mucosal immune responses to protect mice against lethal infection","volume":"30","author":"Hong","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib23","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/IAI.74.1.694-702.2006","article-title":"A\u00a0bacterial flagellin, Vibrio vulnificus FlaB, has a strong mucosal adjuvant activity to induce protective immunity","volume":"74","author":"Lee","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2012.11.039_bib24","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1128\/IAI.05138-11","article-title":"Complete genome sequence of the marine fish pathogen Vibrio anguillarum harboring the pJM1 virulence plasmid and genomic comparison with other virulent strains of V.\u00a0anguillarum and V.\u00a0ordalii","volume":"79","author":"Naka","year":"2011","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2012.11.039_bib25","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.fsi.2010.01.013","article-title":"Cloning and analysis of a ferritin subunit from turbot (Scophthalmus maximus)","volume":"28","author":"Zheng","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib26","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1099\/mic.0.2008\/017343-0","article-title":"Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain","volume":"154","author":"Zhang","year":"2008","journal-title":"Microbiology-SGM"},{"key":"10.1016\/j.fsi.2012.11.039_bib27","doi-asserted-by":"crossref","first-page":"4047","DOI":"10.1016\/j.vaccine.2009.04.023","article-title":"Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine","volume":"27","author":"Wang","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib28","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.fsi.2010.01.001","article-title":"Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus)","volume":"28","author":"Hu","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib29","first-page":"174","article-title":"Suppressor of cytokine signaling 3 inhibits head kidney macrophage activation and cytokine expression in Scophthalmus maximus","volume":"35","author":"Zhang","year":"2011","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib30","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1016\/j.vaccine.2010.07.050","article-title":"Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain","volume":"28","author":"Sun","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0022-1759(90)90029-U","article-title":"Removal of endotoxin from protein solutions by phase separation using Triton X-114","volume":"132","author":"Aida","year":"1990","journal-title":"J\u00a0Immunol Methods"},{"key":"10.1016\/j.fsi.2012.11.039_bib32","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1016\/j.vaccine.2009.11.083","article-title":"Comparative study of the effects of aluminum adjuvants and Freund's incomplete adjuvant on the immune response to an Edwardsiella tarda major antigen","volume":"28","author":"Jiao","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib33","first-page":"447","article-title":"Potency testing of fish vaccines. In: International symposium on fish biologics: serodiagnostics and vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev Biol Stand"},{"key":"10.1016\/j.fsi.2012.11.039_bib34","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0044-8486(97)00070-7","article-title":"The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus","volume":"56","author":"Tong","year":"1997","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2012.11.039_bib35","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.fsi.2010.12.014","article-title":"Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)","volume":"30","author":"Zheng","year":"2011","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib36","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1016\/j.vaccine.2009.06.071","article-title":"Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens","volume":"27","author":"Jiao","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib37","doi-asserted-by":"crossref","first-page":"5635","DOI":"10.1016\/j.vaccine.2010.06.022","article-title":"Dissection and localization of the immunostimulating domain of Edwardsiella tarda FliC","volume":"28","author":"Jiao","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib38","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1128\/IAI.74.2.1113-1120.2006","article-title":"Flagellin is an effective adjuvant for immunization against lethal respiratory challenge with Yersinia pestis","volume":"74","author":"Honko","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.fsi.2012.11.039_bib39","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.vaccine.2007.10.062","article-title":"Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellin","volume":"26","author":"Huleatt","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib40","doi-asserted-by":"crossref","first-page":"3914","DOI":"10.4049\/jimmunol.169.7.3914","article-title":"Bacterial flagellin is an effective adjuvant for CD4+ T cells in vivo","volume":"169","author":"McSorley","year":"2002","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib41","doi-asserted-by":"crossref","first-page":"7678","DOI":"10.1016\/j.vaccine.2011.07.138","article-title":"Immune response of Atlantic salmon to recombinant flagellin","volume":"29","author":"Hynes","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib42","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1002\/eji.200838804","article-title":"Induction of adaptive immunity by flagellin does not require robust activation of innate immunity","volume":"39","author":"Sanders","year":"2009","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib43","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.4049\/jimmunol.175.6.3882","article-title":"Activation of innate immunity, inflammation, and potentiation of DNA vaccination through mammalian expression of the TLR5 agonist flagellin","volume":"175","author":"Applequist","year":"2005","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib44","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1002\/eji.200425403","article-title":"Responses to the soluble flagellar protein FliC are Th2, while those to FliC on Salmonella are Th1","volume":"34","author":"Cunningham","year":"2004","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib45","doi-asserted-by":"crossref","first-page":"3797","DOI":"10.1016\/j.vaccine.2003.12.035","article-title":"Mucosal immunization with purified flagellin from Salmonella induces systemic and mucosal immune responses in C3H\/HeJ mice","volume":"22","author":"Strindelius","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2012.11.039_bib46","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.fsi.2010.06.011","article-title":"Molecular cloning and expression study on Toll-like receptor 5 paralogs in Japanese flounder, Paralichthys olivaceus","volume":"29","author":"Hwang","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2012.11.039_bib47","doi-asserted-by":"crossref","first-page":"48588","DOI":"10.1074\/jbc.M407634200","article-title":"Sensing bacterial flagellin by membrane and soluble orthologs of Toll-like receptor 5 in rainbow trout (Oncorhynchus mykiss)","volume":"279","author":"Tsujita","year":"2004","journal-title":"J\u00a0Biol Chem"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464812004421?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464812004421?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T08:45:10Z","timestamp":1643877910000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464812004421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["S1050464812004421"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2012.11.039","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2013,2]]}}}