{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T09:48:57Z","timestamp":1764841737663},"reference-count":52,"publisher":"Elsevier BV","issue":"2","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2013,8]]},"DOI":"10.1016\/j.fsi.2013.04.050","type":"journal-article","created":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T13:02:38Z","timestamp":1369400558000},"page":"389-398","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of alpha-2-macroglobulin from groupers"],"prefix":"10.1016","volume":"35","author":[{"given":"Wen-Hsiao","family":"Chuang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo-Kau","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping-Chung","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2013.04.050_bib1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0145-305X(01)00038-6","article-title":"Innate host defense mechanisms of fish against viruses and bacteria","volume":"25","author":"Ellis","year":"2001","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib2","first-page":"431","article-title":"Purification and characterization of alpha-2-macroglobulin from the white shrimp (Penaeus vannamei)","volume":"134","author":"Gollas-Galv\u00e1n","year":"2003","journal-title":"Comp Biochem Physiol C"},{"key":"10.1016\/j.fsi.2013.04.050_bib3","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1111\/j.1432-1033.1996.0822r.x","article-title":"Molecular cloning of Limulus alpha 2-macroglobulin","volume":"242","author":"Iwaki","year":"1993","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib4","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.fsi.2004.08.006","article-title":"Molecular cloning of alpha2- macroglobulin in sea scallop Chlamys farreri (Bivalvia, Mollusca)","volume":"18","author":"Ma","year":"2005","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib5","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1042\/bj2050091","article-title":"Evolution of \u03b12-macroglobulin. The demonstration in a variety of vertebrate species of a protein resembling human \u03b12-macroglobulin","volume":"205","author":"Starkey","year":"1982","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2013.04.050_bib6","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1042\/bj2050097","article-title":"Evolution of \u03b12-macroglobulin. The purification and characterisation of a protein homologous with human \u03b12-macroglobulin from plaice (Pleuronectes platessa L.) plasma","volume":"205","author":"Starkey","year":"1982","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2013.04.050_bib7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1042\/bj2050105","article-title":"Evolution of \u03b12-macroglobulin. The structure of a protein homologous with human \u03b12-macroglobulin from plaice (Pleuronectes platessa L.) plasma","volume":"205","author":"Starkey","year":"1982","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2013.04.050_bib8","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1042\/bj1330709","article-title":"The interaction of \u03b12-macroglobulin with proteinases, characteristics and specificity of the reaction and hypothesis concerning its molecular mechanism","volume":"133","author":"Barrett","year":"1973","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2013.04.050_bib9","doi-asserted-by":"crossref","first-page":"5700","DOI":"10.1073\/pnas.82.17.5700","article-title":"Model of alpha 2-macroglobulin structure and function","volume":"82","author":"Feldman","year":"1985","journal-title":"Proc Natl Acad Sci U.S.A"},{"key":"10.1016\/j.fsi.2013.04.050_bib10","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0882-4010(87)90093-3","article-title":"Inhibition of the Aeromonas salmonicida extracellular proteases by\u00a0\u03b12-macroglobulin in the serum of rainbow trout","volume":"3","author":"Ellis","year":"1987","journal-title":"Microb Pathog"},{"key":"10.1016\/j.fsi.2013.04.050_bib11","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0305-0491(91)90252-9","article-title":"The role of \u03b12-macroglobulin in furunculosis: a comparison of rainbow trout and brook trout","volume":"98","author":"Freedman","year":"1991","journal-title":"Comp Biochem Physiol B"},{"key":"10.1016\/j.fsi.2013.04.050_bib12","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3354\/dao029067","article-title":"In\u00a0vivo neutralization of Cryptobia salmositica metallo-protease by alpha-2-macroglobulin in the blood of rainbow trout Oncorhynchus mykiss and brook charr Salvelinus fonrizalis","volume":"29","author":"Zuo","year":"1997","journal-title":"Dis Aquat Org"},{"key":"10.1016\/j.fsi.2013.04.050_bib13","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s002510000216","article-title":"Multiple forms of \u03b12-macroglobulin from a bony fish, the common carp (Cyprinus carpio): striking sequence diversity in functional sites","volume":"51","author":"Mutsuro","year":"2000","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2013.04.050_bib14","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.cbpb.2005.06.010","article-title":"Alpha2-macroglobulin in the marine fish Sparus aurata","volume":"141","author":"Funkenstein","year":"2005","journal-title":"Comp Biochem Physiol A"},{"key":"10.1016\/j.fsi.2013.04.050_bib15","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.fsi.2005.06.006","article-title":"Purification and characterization of \u03b12-macroglobulin from grass carp Ctenopharyngodon idellus: cloning a segment of the corresponding gene","volume":"20","author":"Li","year":"2006","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib16","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.aquaculture.2008.07.053","article-title":"Purification and characterization of an alpha-2-macroglobulin protease inhibitor from plasma of the grouper Epinephelus coioides","volume":"284","author":"Chuang","year":"2008","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2013.04.050_bib17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.fsi.2010.03.010","article-title":"Molecular cloning and\u00a0characterization of alpha 2-macroglobulin (\u03b12-M) from the haemocytes ofChinese mitten crab Eriocheir sinensis","volume":"29","author":"Qin","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.fsi.2006.03.017","article-title":"Molecular cloning and characterisation of a thioester-containing \u03b12-macroglobulin (\u03b12-M) from the\u00a0haemocytes of mud crab Scylla serrata","volume":"22","author":"Vaseeharan","year":"2007","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib19","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.dci.2007.07.002","article-title":"Molecular cloning and phylogenetic analysison \u03b1 2-macroglobulin (\u03b12-M) of white shrimp Litopenaeus vannamei","volume":"32","author":"Lin","year":"2008","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib20","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.molimm.2006.08.002","article-title":"Molecular cloning and characterisation of a proteinase inhibitor, alpha 2-macroglobulin (\u03b12-M) from the haemocytes of tiger shrimp Penaeus monodon","volume":"44","author":"Lin","year":"2007","journal-title":"Mol Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib21","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.fsi.2003.09.011","article-title":"Molecular cloning and expression analysis of \u03b12-macroglobulin in the kuruma shrimp, Marsupenaeus japonicus","volume":"15","author":"Rattanachai","year":"2004","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib22","series-title":"Diseases of cultured groupers","first-page":"81","author":"Nagasawa","year":"2004"},{"key":"10.1016\/j.fsi.2013.04.050_bib23","series-title":"Economics and marketing of the live reef fish trade in Asia-Pacific","first-page":"116","article-title":"Aquaculture of groupers in Asia and the Pacific","author":"Rimmer","year":"2006"},{"key":"10.1016\/j.fsi.2013.04.050_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0044-8486(01)00692-5","article-title":"Techniques in finfish larviculture in Taiwan","volume":"200","author":"Liao","year":"2001","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2013.04.050_bib25","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1006\/fsim.1997.0092","article-title":"Serum trypsin-inhibitory activity in five species of farmed fish","volume":"7","author":"Bowden","year":"1997","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib26","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A\u00a0rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib27","series-title":"Techniques in fish immunology. Fish immunology technical communications 1","first-page":"95","article-title":"Serum anti-proteases in fish: \u03b12-macroglobulin assay","author":"Ellis","year":"1990"},{"key":"10.1016\/j.fsi.2013.04.050_bib28","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1146\/annurev.bi.62.070193.000433","article-title":"Analysis of\u00a0glycoprotein-associated oligosaccharides","volume":"62","author":"Dwek","year":"1993","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib29","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1021\/bi00053a037","article-title":"Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins","volume":"32","author":"Patel","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2013.04.050_bib30","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(81)90168-8","article-title":"Deglycosylation of glycoproteins by trifluoromethanesulfonic acid","volume":"118","author":"Edge","year":"1981","journal-title":"Anal Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib31","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1042\/bj20030673","article-title":"Deglycosylation of glycoproteins with trifluoromethanesulphonic acid: elucidation of molecular structure and function","volume":"376","author":"Edge","year":"2003","journal-title":"Biochem J"},{"key":"10.1016\/j.fsi.2013.04.050_bib32","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s00436-005-1340-8","article-title":"Identification of immune genes in grass carp Ctenopharyngodon idellus in response to infection of the parasitic copepod Sinergasilus major","volume":"96","author":"Chang","year":"2005","journal-title":"Parasitol Res"},{"year":"2003","author":"Lai","series-title":"Evolution of complement and \u03b12-macroglobulin in lower vertebrates: Isolation of the cDNA clone specific for the lamprey protein homologous to \u03b12-macroglobulin","key":"10.1016\/j.fsi.2013.04.050_bib33"},{"key":"10.1016\/j.fsi.2013.04.050_bib34","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1046\/j.1365-3083.2002.01154.x","article-title":"Linkage relationships of genes coding for alpha2-macroglobulin, C3 and C4 in the zebrafish: implications for the evolution of the complement and Mhc systems","volume":"56","author":"Samonte","year":"2002","journal-title":"Scand J Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib35","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1023\/A:1023076029496","article-title":"Molecular cloning and structural characterization of the hagfish proteinase inhibitor of the \u03b12-macroglobulin family","volume":"22","author":"Idiris","year":"2003","journal-title":"J\u00a0Protein Chem"},{"key":"10.1016\/j.fsi.2013.04.050_bib36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/0009-8981(66)90037-4","article-title":"Determination of \u03b12-macroglobulin as trypsin-protein esterase","volume":"14","author":"Ganrot","year":"1966","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.fsi.2013.04.050_bib37","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1046\/j.1432-1327.2000.01020.x","article-title":"Characterization of an \u03b1-macroglobulin-like glycoprotein isolated from the plasma of the soft tick Ornithodoros moubata","volume":"267","author":"Kop\u00e1\u010dek","year":"2000","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib38","doi-asserted-by":"crossref","first-page":"8318","DOI":"10.1016\/S0021-9258(17)39730-2","article-title":"Primary structure of human alpha-2-macroglobulin. V. The complete structure","volume":"259","author":"Sottrup-Jensen","year":"1984","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.fsi.2013.04.050_bib39","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/PL00011749","article-title":"Purification and characterization of \u03b1-1-proteinase inhibitor from carp (Cyprinus carpio) serum","volume":"1","author":"Aranishi","year":"1999","journal-title":"Mar Biotechnol"},{"key":"10.1016\/j.fsi.2013.04.050_bib40","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/oxfordjournals.jbchem.a003186","article-title":"Affinity capturing and gene assignment of soluble glycoproteins produced by the nematode Caenorhabditis elegans","volume":"132","author":"Hirabayashi","year":"2002","journal-title":"J\u00a0Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib41","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.chroma.2004.08.150","article-title":"Approach to the comprehensive analysis of glycoproteins isolated from human serum using a multi-lectin affinity column","volume":"1053","author":"Yang","year":"2004","journal-title":"J\u00a0Chromatogr A"},{"key":"10.1016\/j.fsi.2013.04.050_bib42","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1006\/fsim.1997.0135","article-title":"Immunological cross reactions between Aeromonas salmonicida and Vibrio salmonicida in Atlantic salmon (Salmo salar L.) and rabbit","volume":"8","author":"Hoel","year":"1998","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib43","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1006\/meth.1999.0856","article-title":"Electrophoretic methods for studying protein-protein interactions","volume":"19","author":"Vergnon","year":"1999","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2013.04.050_bib44","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1093\/oxfordjournals.jbchem.a129313","article-title":"Studies on alpha 2-macroglobulin in bovine plasma. I. Purification, physical properties, and chemical compositions","volume":"67","author":"Nagasawa","year":"1970","journal-title":"J\u00a0Biochem"},{"key":"10.1016\/j.fsi.2013.04.050_bib45","series-title":"The plasma proteins: structure, function and genetic control","first-page":"191","article-title":"Alpha-2-Macroglobulin and related thiol ester plasma proteins","author":"Sottrup-Jensen","year":"1987"},{"key":"10.1016\/j.fsi.2013.04.050_bib46","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1006\/fsim.2001.0408","article-title":"The complement system in teleosts","volume":"12","author":"Claire","year":"2002","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2013.04.050_bib47","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s002510050020","article-title":"Molecular cloning and linkage analysis of complement C3 and C4 genes of the Japanese medaka fish","volume":"51","author":"Kuroda","year":"2000","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2013.04.050_bib48","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1749-6632.1994.tb44316.x","article-title":"The proteinase-binding reaction of alpha 2M","volume":"737","author":"Feinman","year":"1994","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.fsi.2013.04.050_bib49","first-page":"792","article-title":"Alpha 2-macroglobulin, complement, and biologic defense: antigens, growth factors, microbial proteases, and receptor ligation","volume":"71","author":"Chu","year":"1994","journal-title":"Lab Invest"},{"key":"10.1016\/j.fsi.2013.04.050_bib50","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1016\/S0021-9258(17)42289-7","article-title":"Classification of alpha 2-macroglobulin-cytokine interactions based on affinity of noncovalent association in solution under apparent equilibrium conditions","volume":"269","author":"Crookston","year":"1994","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.fsi.2013.04.050_bib51","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1749-6632.1994.tb44318.x","article-title":"Alpha 2-macroglobulin and the alpha 2-macroglobulin receptor\/LRP. A growth regulatory axis","volume":"737","author":"Gonias","year":"1994","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.fsi.2013.04.050_bib52","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/S0020-7519(01)00169-2","article-title":"Cryptobiosis and its control in North American fishes","volume":"31","author":"Woo","year":"2001","journal-title":"Int J Parasitol"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464813005536?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464813005536?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T16:44:46Z","timestamp":1645548286000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464813005536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["S1050464813005536"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2013.04.050","relation":{},"ISSN":["1050-4648"],"issn-type":[{"type":"print","value":"1050-4648"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of alpha-2-macroglobulin from groupers","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2013.04.050","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}