{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T14:50:04Z","timestamp":1783522204446,"version":"3.55.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Funds of China","doi-asserted-by":"crossref","award":["31372550"],"award-info":[{"award-number":["31372550"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National High Technology Research and Development Program of China","award":["2014AA093604"],"award-info":[{"award-number":["2014AA093604"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2014,10]]},"DOI":"10.1016\/j.fsi.2014.07.011","type":"journal-article","created":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:16:34Z","timestamp":1406247394000},"page":"354-361","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Molecular cloning and characterization of secretory and membrane-bound IgM of turbot"],"prefix":"10.1016","volume":"40","author":[{"given":"Yuan","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haizhen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangbo","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanxing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2014.07.011_bib1","first-page":"91","article-title":"The immune system of cyprinid fish. Kinetics and temperature dependence of antibody-producing cells in carp (Cyprinus carpio)","volume":"41","author":"Rijkers","year":"1980","journal-title":"Immunology"},{"key":"10.1016\/j.fsi.2014.07.011_bib2","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1006\/fsim.1998.0149","article-title":"Isolation of cDNA encoding the constant region of the immunoglobulin heavy chain from common carp (Cyprinus carpio L)","volume":"8","author":"Nakao","year":"1998","journal-title":"Fish shellfish Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib3","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1007\/s00251-004-0693-y","article-title":"Fugu immunoglobulin D: a highly unusual gene with unprecedented duplications in its constant region","volume":"56","author":"Saha","year":"2004","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2014.07.011_bib4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S1050-4648(02)00139-0","article-title":"Cloning and characterisation of a cDNA encoding Japanese flounder Paralichthys olivaceus IgD","volume":"15","author":"Hirono","year":"2003","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib5","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.4049\/jimmunol.169.5.2488","article-title":"The IgH locus of the channel catfish, Ictalurus punctatus, contains multiple constant region gene sequences: different genes encode heavy chains of membrane and secreted IgD","volume":"169","author":"Bengten","year":"2002","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib6","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.4049\/jimmunol.1101938","article-title":"Discovery and characterization of secretory IgD in rainbow trout: secretory IgD is produced through a novel splicing mechanism","volume":"188","author":"F1","year":"2012","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib7","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.dci.2009.08.011","article-title":"Presence of a unique IgT on the IGH locus in Gasterosteus aculeatus and the very recent generation of a repertoire of VH genes","volume":"34","author":"Deza","year":"2009","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib8","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.molimm.2009.10.010","article-title":"Identification and characterization of a novel immunoglobulin Z isotype in zebrafishes: implications for a distinct B cell receptor in lower vertebrates","volume":"47","author":"Hu","year":"2010","journal-title":"Mol Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib9","series-title":"Handbook of vertebrate immunology","first-page":"1","article-title":"Immunology of fishes","author":"Rombout","year":"1998"},{"key":"10.1016\/j.fsi.2014.07.011_bib10","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.cbpb.2005.10.003","article-title":"Antibody structural variation in rainbow trout fluids","volume":"143","author":"Bromage","year":"2006","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"10.1016\/j.fsi.2014.07.011_bib11","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1007\/s00251-004-0689-7","article-title":"Genes of the constant region of functional immunoglobulin heavy chain of Japanese flounder Paralychthys olivaceus","volume":"56","author":"Srisapoome","year":"2004","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2014.07.011_bib12","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1007\/s00251-005-0015-z","article-title":"Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.)","volume":"57","author":"R1","year":"2005","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2014.07.011_bib13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.aquaculture.2008.11.023","article-title":"Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuuatsi lymphoid tissues and their transcription changes after Flavobacterium columnare stimulation","volume":"288","author":"Tian","year":"2009","journal-title":"Aquaculture"},{"issue":"9","key":"10.1016\/j.fsi.2014.07.011_bib14","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/ni.1913","article-title":"IgT, a primitive immunoglobulin class specialized in mucosal immunity","volume":"11","author":"Zhang","year":"2010","journal-title":"Nat Immunol"},{"issue":"7","key":"10.1016\/j.fsi.2014.07.011_bib15","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S0145-305X(02)00012-5","article-title":"Characterisation of immunoglobulin light chain cDNA of the Atlantic salmon, Salmo salar L., evidence for three IgL isotypes","volume":"26","author":"Solem","year":"2002","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0145-305X(90)90008-3","article-title":"Cellular requirements for lymphokine secretion by rainbow trout Salmo gairdneri leucocytes","volume":"30","author":"Graham","year":"1990","journal-title":"Dev Comp Immunol"},{"issue":"4","key":"10.1016\/j.fsi.2014.07.011_bib17","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10126-010-9279-x","article-title":"Immunological control of fish diseases","volume":"12","author":"Magnadottir","year":"2010","journal-title":"Mar Biotechnol"},{"key":"10.1016\/j.fsi.2014.07.011_bib18","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0044-8486(97)00114-2","article-title":"The use of immunostimulation in marine larviculture: possibilities and challenges","volume":"155","author":"Vadstein","year":"1997","journal-title":"Aquaculture"},{"issue":"6","key":"10.1016\/j.fsi.2014.07.011_bib19","doi-asserted-by":"crossref","first-page":"e20970","DOI":"10.1371\/journal.pone.0020970","article-title":"Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri","volume":"6","author":"\u00c1lves","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.fsi.2014.07.011_bib20","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/s10126-009-9203-4","article-title":"The effects of immunostimulation through dietary manipulation in the rainbow trout evaluation of mucosal immunity","volume":"12","author":"Do\u00f1ate","year":"2010","journal-title":"Mar Biotechnol"},{"issue":"5","key":"10.1016\/j.fsi.2014.07.011_bib21","doi-asserted-by":"crossref","first-page":"e35369","DOI":"10.1371\/journal.pone.0035369","article-title":"High-throughput sequence analysis of turbot (Scophthalmus maximus) transcriptome using 454-pyrosequencing for the discovery of antiviral immune genes","volume":"7","author":"Pereiro","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2014.07.011_bib22","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1186\/1471-2164-14-180","article-title":"A\u00a0combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (Scophthalmus maximus)","volume":"14","author":"Ribas","year":"2013","journal-title":"BMC Genomics"},{"key":"10.1016\/j.fsi.2014.07.011_bib23","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fsi.2012.03.031","article-title":"Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate","volume":"33","author":"Zhang","year":"2012","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib24","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Thomas","year":"2008","journal-title":"Nat Protoc"},{"key":"10.1016\/j.fsi.2014.07.011_bib25","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1006\/fsim.2001.0336","article-title":"Flounder (Paralichthys olivaceus) cDNA encoding a secreted immunoglobulin M heavy chain","volume":"11","author":"Lee","year":"2001","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib26","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1046\/j.1365-3083.1999.00583.x","article-title":"Molecular cloning and phylogenetic analysis of the Atlantic salmon immunoglobulin D gene","volume":"50","author":"Hordvik","year":"1999","journal-title":"Scand J Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib27","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.4049\/jimmunol.169.5.2488","article-title":"The IgH locus of the channel catfish, Ictalurus punctatus, contains multiple constant region gene sequences: different genes encode heavy chains of membrane and secreted IgD","volume":"169","author":"Bengten","year":"2002","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib28","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1038\/296410a0","article-title":"Structure of genes for membrane and secreted murine IgD heavy chains","volume":"296","author":"Cheng","year":"1982","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2014.07.011_bib29","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.4049\/jimmunol.171.3.1312","article-title":"The porcine Ig delta gene: unique chimeric splicing of the first constant region domain in its heavy chain transcripts","volume":"171","author":"Zhao","year":"2003","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib30","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1126\/science.3922054","article-title":"Human immunoglobulin D: genomic sequence of the delta heavy chain","volume":"228","author":"White","year":"1985","journal-title":"Science"},{"key":"10.1016\/j.fsi.2014.07.011_bib31","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.dci.2005.06.007","article-title":"Antibody repertoire development in teleosts\u2013a review with emphasis on salmonids and Gadus morhua L","volume":"30","author":"Solem","year":"2006","journal-title":"Dev Comp Immunol"},{"issue":"1","key":"10.1016\/j.fsi.2014.07.011_bib32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1600-065X.1998.tb01263.x","article-title":"Functional and molecular characterization of teleost leukocytes","volume":"166","author":"Miller","year":"1998","journal-title":"Immunol Rev"},{"issue":"3","key":"10.1016\/j.fsi.2014.07.011_bib33","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1084\/jem.135.3.660","article-title":"Receptors on immunocompetent cells. V. cellular correlates of \u201cmaturation\u201d of the immune response","volume":"135","author":"Davie","year":"1972","journal-title":"J\u00a0Exp Med"},{"key":"10.1016\/j.fsi.2014.07.011_bib34","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.vetimm.2008.09.022","article-title":"Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)","volume":"127","author":"Feng","year":"2009","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.fsi.2014.07.011_bib35","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.vetimm.2005.08.029","article-title":"Characterization of serum immunoglobulin M of grouper and cDNAcloning of its heavy chain","volume":"109","author":"Cheng","year":"2006","journal-title":"Vet Immunol Immunopathol"},{"issue":"4","key":"10.1016\/j.fsi.2014.07.011_bib36","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.fsi.2012.06.011","article-title":"Characterization of RAG1 and IgM (mu chain) marking development of the immune system in red-spotted grouper (Epinephelus akaara)","volume":"33","author":"Mao","year":"2012","journal-title":"Fish Shellfish Immunol"},{"issue":"5","key":"10.1016\/j.fsi.2014.07.011_bib37","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.fsi.2005.03.010","article-title":"Ontogeny of humoral immune parameters in fish","volume":"19","author":"Magnadottir","year":"2005","journal-title":"Fish Shellfish Immunol"},{"issue":"1","key":"10.1016\/j.fsi.2014.07.011_bib38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetimm.2010.07.024","article-title":"Molecular cloning of IgT from Atlantic salmon, and analysis of the relative expression of \u03c4, \u03bc and \u03b4 in different tissues","volume":"139","author":"Tadiso","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"issue":"1","key":"10.1016\/j.fsi.2014.07.011_bib39","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.vetimm.2008.12.026","article-title":"Response of mucosal and systemic sIgM-positive cells in turbot (Scophthalmus maximus L.) immunization with Edwardsiella tarda","volume":"129","author":"Feng","year":"2009","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.fsi.2014.07.011_bib40","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.fsi.2005.03.007","article-title":"Phylogeny and ontogeny of fish leucocytes","volume":"19","author":"Rombout","year":"2005","journal-title":"Fish Shellfish Immunol"},{"issue":"19","key":"10.1016\/j.fsi.2014.07.011_bib41","doi-asserted-by":"crossref","first-page":"6919","DOI":"10.1073\/pnas.0500027102","article-title":"Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: implications for a distinctive B cell developmental pathway in teleost fish","volume":"102","author":"Hansen","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"10.1016\/j.fsi.2014.07.011_bib42","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.fsi.2004.03.005","article-title":"Monoclona antibodies recognising serum immunoglobulins and surface immunoglobulin-positive cells of puffer fish, torafugu (Takifugu rubripes)","volume":"17","author":"Miyadai","year":"2004","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.fsi.2014.07.011_bib43","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1038\/ni1389","article-title":"B\u00a0lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities","volume":"7","author":"Li","year":"2006","journal-title":"Nat Immunol"},{"issue":"1","key":"10.1016\/j.fsi.2014.07.011_bib44","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.fsi.2009.10.021","article-title":"Phagocytosis by B-cells and neutrophils in Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.)","volume":"28","author":"\u00d8verland","year":"2010","journal-title":"Fish Shellfish Immunol"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464814002526?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464814002526?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T05:10:47Z","timestamp":1565673047000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464814002526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["S1050464814002526"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2014.07.011","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2014,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning and characterization of secretory and membrane-bound IgM of turbot","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2014.07.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}