{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T16:35:13Z","timestamp":1781368513176,"version":"3.54.1"},"reference-count":48,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100007273","name":"Comisi\u00f3n Interministerial de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["AGL2010-21219\/ACU"],"award-info":[{"award-number":["AGL2010-21219\/ACU"]}],"id":[{"id":"10.13039\/501100007273","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007273","name":"Comisi\u00f3n Interministerial de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["AGL2011-29927-C02-01"],"award-info":[{"award-number":["AGL2011-29927-C02-01"]}],"id":[{"id":"10.13039\/501100007273","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"crossref","award":["R2014\/046"],"award-info":[{"award-number":["R2014\/046"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Aquagenomics","award":["INCITE07\/PX1203039ES"],"award-info":[{"award-number":["INCITE07\/PX1203039ES"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2015,6]]},"DOI":"10.1016\/j.fsi.2015.03.034","type":"journal-article","created":{"date-parts":[[2015,4,6]],"date-time":"2015-04-06T06:31:10Z","timestamp":1428301870000},"page":"652-661","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs"],"prefix":"10.1016","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0763-2548","authenticated-orcid":false,"given":"I.","family":"Folgueira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Noia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Blanco-Abad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Mallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Leiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lamas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2015.03.034_bib1","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1038\/nm.3409","article-title":"Key roles of adjuvants in modern vaccines","volume":"19","author":"Reed","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.fsi.2015.03.034_bib2","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1016\/j.fsi.2013.02.029","article-title":"Adjuvants and immunostimulants in fish vaccines: current knowledge and future perspectives","volume":"35","author":"Tafalla","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib3","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.fsi.2003.10.002","article-title":"Sequential study of antigen persistence and concomitant inflammatory reactions relative to side-effects and growth of Atlantic salmon (Salmo salar L.) following intraperitoneal injection with oil-adjuvanted vaccines","volume":"16","author":"Mutoloki","year":"2004","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1365-2761.2004.00583.x","article-title":"Vaccine-associated granulomatous inflammation and melanin accumulation in Atlantic salmon, Salmo salar L., white muscle","volume":"28","author":"Koppang","year":"2005","journal-title":"J.\u00a0Fish Dis."},{"key":"10.1016\/j.fsi.2015.03.034_bib5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.fsi.2011.11.003","article-title":"A\u00a0sequential study of incomplete Freund's adjuvant-induced peritonitis in Atlantic cod","volume":"32","author":"Gjessing","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib6","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.fsi.2014.03.020","article-title":"Inflammatory responses and side effects generated by several adjuvant-containing vaccines in turbot","volume":"38","author":"Noia","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib7","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.vaccine.2011.10.105","article-title":"PLGA\/PLA micro- and nanoparticle formulations serve as antigen depots and induce elevated humoral responses after immunization of Atlantic salmon (Salmo salar L.)","volume":"30","author":"Fredriksen","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2015.03.034_bib8","doi-asserted-by":"crossref","first-page":"5278","DOI":"10.1016\/j.vaccine.2012.05.028","article-title":"Antigen adsorbed surface modified poly-\u025b-caprolactone microspheres stimulates both adaptive and innate immune response in fish","volume":"30","author":"Behera","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2015.03.034_bib9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.fsi.2012.03.028","article-title":"A\u00a0vaccine based on biodegradable microspheres induces protective immunity against scuticociliatosis without producing side effects in turbot","volume":"33","author":"Le\u00f3n-Rodr\u00edguez","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib10","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/nrmicro1681","article-title":"The perfect mix: recent progress in adjuvant research","volume":"5","author":"Guy","year":"2007","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2015.03.034_bib11","doi-asserted-by":"crossref","first-page":"64","DOI":"10.4103\/2230-973X.82384","article-title":"Recent trends in vaccine delivery systems: a review","volume":"1","author":"Saroja","year":"2011","journal-title":"Int. J. Pharm. Investig."},{"key":"10.1016\/j.fsi.2015.03.034_bib12","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1002\/jbm.10019","article-title":"Uptake of poly(D,L-lactic-coglycolic acid) microspheres by antigen-presenting cells in\u00a0vivo","volume":"60","author":"Newman","year":"2002","journal-title":"J.\u00a0Biomed Mater Res."},{"key":"10.1016\/j.fsi.2015.03.034_bib13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/bj20031253","article-title":"Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis","volume":"377","author":"Rejman","year":"2004","journal-title":"Biochem. J."},{"key":"10.1016\/j.fsi.2015.03.034_bib14","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1038\/ni1389","article-title":"B\u00a0lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities","volume":"7","author":"Li","year":"2006","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib15","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1189\/jlb.0711372","article-title":"Pivotal advance: peritoneal cavity B-1 B cells have phagocytic and microbicidal capacities and present phagocytosed antigen to CD4+ T cells","volume":"91","author":"Parra","year":"2012","journal-title":"J.\u00a0Leukoc. Biol."},{"key":"10.1016\/j.fsi.2015.03.034_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fsi.2004.09.001","article-title":"Side effects in sea bass (Dicentrarchus labrax L.) due to intraperitoneal vaccination against vibriosis and pasteurellosis","volume":"19","author":"Afonso","year":"2005","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s00441-004-1063-7","article-title":"Early innate immune response and redistribution of inflammatory cells in the bony fish gilthead seabream experimentally infected with Vibrio anguillarum","volume":"320","author":"Chaves-Pozo","year":"2005","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.fsi.2015.03.034_bib18","first-page":"101","article-title":"Localization of immune complexes and heat-aggregated immunoglobulin in the carp Cyprinus carpio L","volume":"47","author":"Secombes","year":"1982","journal-title":"Immunology"},{"key":"10.1016\/j.fsi.2015.03.034_bib19","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1365-2761.1995.tb00295.x","article-title":"Antigen retention and enzyme reactivity in the spleen of Atlantic salmon, Salmo salar L., following administration of injectable furunculosis vaccines","volume":"18","author":"Press CMcL","year":"1995","journal-title":"J.\u00a0Fish Dis."},{"key":"10.1016\/j.fsi.2015.03.034_bib20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1006\/fsim.2001.0390","article-title":"Antigen uptake and immunoglobulin production in Atlantic cod (Gadus morhua L.) after intraperitoneal injection of Vibrio anguillarum","volume":"13","author":"Arnesen","year":"2002","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib21","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S1050-4648(02)00184-5","article-title":"Evensen \u00d8. Distribution and retention of antigens of Aeromonas salmonicida in Atlantic salmon (Salmo salar L.) vaccinated with a DeltaaroA mutant or formalin-inactivated bacteria in oil-adjuvant","volume":"15","author":"Grove","year":"2003","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/(SICI)1097-0185(199602)244:2<235::AID-AR11>3.0.CO;2-Q","article-title":"Omental milky spots in the local immune response in the peritoneal cavity in rats","volume":"244","author":"Van Vugt","year":"1996","journal-title":"Anat. Rec."},{"key":"10.1016\/j.fsi.2015.03.034_bib23","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.4049\/jimmunol.0900409","article-title":"Lymphocytes in the peritoneum home to the omentum and are activated by resident dendritic cells","volume":"183","author":"Carlow","year":"2009","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib24","first-page":"114","article-title":"Lymph flow and lymphatic drainage of inflammatory cells from the peritoneal cavity in a casein-peritonitis model in sheep","volume":"27","author":"Yuan","year":"1994","journal-title":"Lymphology"},{"key":"10.1016\/j.fsi.2015.03.034_bib25","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/oxfordjournals.jbchem.a129077","article-title":"Reaction of fluorescein-isothiocyanate with proteins and amino acids. I. Covalent and non-covalent binding of fluorescein-isothiocyanate and fluorescein to proteins","volume":"65","author":"Maeda","year":"1969","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.fsi.2015.03.034_bib26","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.chemphyslip.2012.03.007","article-title":"Uptake of a fluorescent methyl-\u03b2-cyclodextrin via clathrin-dependent endocytosis","volume":"165","author":"Plazzo","year":"2012","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.fsi.2015.03.034_bib27","series-title":"Histological and Histochemical Methods","author":"Kiernan","year":"1981"},{"key":"10.1016\/j.fsi.2015.03.034_bib28","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/0008-8749(89)90367-5","article-title":"Kinetics of changes in peritoneal cell populations following acute inflammation","volume":"118","author":"Melnicoff","year":"1989","journal-title":"Cell. Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib29","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1006\/fsim.1997.0089","article-title":"The leucocyte population of the unstimulated peritoneal cavity of rainbow trout (Oncorhynchus mykiss)","volume":"7","author":"Afonso","year":"1997","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib30","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.fsi.2006.05.005","article-title":"Early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells","volume":"22","author":"Cuesta","year":"2007","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib31","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.dci.2009.05.009","article-title":"Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish","volume":"33","author":"Moss","year":"2009","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib32","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.fsi.2013.07.032","article-title":"Novel insights into the peritoneal inflammation of rainbow trout (Oncorhynchus mykiss)","volume":"35","author":"Koryt\u00e1\u0159","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib33","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1002\/jmor.10256","article-title":"Development of the islets, exocrine pancreas, and related ducts in the Nile tilapia, Oreochromis niloticus (Pisces: Cichlidae)","volume":"261","author":"Morrison","year":"2004","journal-title":"J.\u00a0Morphol."},{"key":"10.1016\/j.fsi.2015.03.034_bib34","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1242\/dev.077701","article-title":"Lyve1 expression reveals novel lymphatic vessels and new mechanisms for lymphatic vessel development in zebrafish","volume":"139","author":"Okuda","year":"2012","journal-title":"Development"},{"key":"10.1016\/j.fsi.2015.03.034_bib35","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.4049\/jimmunol.157.6.2577","article-title":"In\u00a0vivo fate of the inflammatory macrophage during the resolution of inflammation: inflammatory macrophages do not die locally, but emigrate to the draining lymph nodes","volume":"157","author":"Bellingan","year":"1996","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib36","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.immuni.2009.04.005","article-title":"Lymphoid organs for peritoneal cavity immune response: milky spots","volume":"30","author":"Mebius","year":"2009","journal-title":"Immunity"},{"key":"10.1016\/j.fsi.2015.03.034_bib37","first-page":"5400","article-title":"Activation of omental milky spots and milky spot macrophages by intraperitoneal administration of a streptococcal preparation, OK-432","volume":"52","author":"Shimotsuma","year":"1992","journal-title":"Cancer Res."},{"key":"10.1016\/j.fsi.2015.03.034_bib38","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.4049\/jimmunol.180.4.2196","article-title":"Differential molecular and anatomical basis for B cell migration into the peritoneal cavity and omental milky spots","volume":"180","author":"Berberich","year":"2008","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib39","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0145-305X(91)90007-L","article-title":"The use of fluorescent microspheres in the study of piscine macrophage aggregate kinetics","volume":"15","author":"Ziengenfuss","year":"1991","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib40","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.bbrc.2008.10.011","article-title":"Neutrophils and monocytes transport tumor cell antigens from the peritoneal cavity to secondary lymphoid tissues","volume":"377","author":"Terasawa","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fsi.2015.03.034_bib41","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1016\/j.vaccine.2010.12.090","article-title":"Vaccine adjuvants alum and MF59 induce rapid recruitment of neutrophils and monocytes that participate in antigen transport to draining lymph nodes","volume":"29","author":"Calabro","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2015.03.034_bib42","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1084\/jem.20011794","article-title":"Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation","volume":"196","author":"Bellingan","year":"2002","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.fsi.2015.03.034_bib43","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/smim.2000.0253","article-title":"Localization dose and time of antigens determine immune reactivity","volume":"12","author":"Zinkernagel","year":"2000","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.fsi.2015.03.034_bib44","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1586\/erv.10.89","article-title":"Nano-microparticles as immune adjuvants: correlating particle sizes and the resultant immune responses","volume":"9","author":"Oyewumi","year":"2010","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.fsi.2015.03.034_bib45","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1208\/s12248-012-9418-6","article-title":"Biodegradable particles as vaccine delivery systems: size matters","volume":"15","author":"Joshi","year":"2013","journal-title":"AAPS J."},{"key":"10.1016\/j.fsi.2015.03.034_bib46","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.ijpharm.2005.05.028","article-title":"Influence of particle size, antigen load, dose and additional adjuvant on the immune response from antigen loaded PLA microparticles","volume":"301","author":"Katare","year":"2005","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.fsi.2015.03.034_bib47","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0264-410X(98)00323-5","article-title":"Biodegradable microspheres containing influenza A vaccine: immune response in mice","volume":"17","author":"Hilbert","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2015.03.034_bib48","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.fsi.2013.06.030","article-title":"Transgene and immune gene expression following intramuscular injection of Atlantic salmon (Salmo salar L.) with DNA-releasing PLGA nano- and microparticles","volume":"35","author":"H\u00f8lvold","year":"2013","journal-title":"Fish Shellfish Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464815001436?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464815001436?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T06:48:44Z","timestamp":1691563724000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464815001436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["S1050464815001436"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2015.03.034","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2015,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2015.03.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}