{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T16:21:51Z","timestamp":1768926111698,"version":"3.49.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Earmarked Fund","award":["CARS-46-11"],"award-info":[{"award-number":["CARS-46-11"]}]},{"name":"Wuhan Science and Technology Plan Grant, Hubei Province","award":["2014020202010138"],"award-info":[{"award-number":["2014020202010138"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2016,2]]},"DOI":"10.1016\/j.fsi.2016.01.003","type":"journal-article","created":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T08:29:20Z","timestamp":1452068960000},"page":"344-350","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"special_numbering":"C","title":["Immune response and protection in gibel carp, Carassius gibelio, after vaccination with \u03b2-propiolactone inactivated cyprinid herpesvirus 2"],"prefix":"10.1016","volume":"49","author":[{"given":"Linlin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuding","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenzhi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zemao","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingbing","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2016.01.003_bib1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0044-8486(00)00602-5","article-title":"Effect of several feeding stimulants on diet preference by juvenile gibel carp (Carassius auratus gibelio), fed diets with or without partial replacement of fish meal by meat and bone meal","volume":"198","author":"Xue","year":"2001","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2016.01.003_bib2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.vetmic.2012.10.033","article-title":"Cyprinid herpesvirus 3 infection disrupts the skin barrier of common carp (Cyprinus carpio L.)","volume":"162","author":"Adamek","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib3","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.3390\/v3112160","article-title":"Herpesviruses that infect fish","volume":"3","author":"Hanson","year":"2011","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2016.01.003_bib4","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00705-008-0278-4","article-title":"The order Herpesvirales","volume":"154","author":"Davison","year":"2009","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2016.01.003_bib5","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1099\/vir.0.80982-0","article-title":"Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae","volume":"86","author":"Waltzek","year":"2005","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.fsi.2016.01.003_bib6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1365-2761.1995.tb00296.x","article-title":"Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.)","volume":"18","author":"Jung","year":"1995","journal-title":"J.\u00a0Fish. Dis."},{"key":"10.1016\/j.fsi.2016.01.003_bib7","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3147\/jsfp.34.209","article-title":"Epizootic of herpeslike virus infection in goldfish, Carassius auratus, in Taiwan","volume":"34","author":"Chang","year":"1999","journal-title":"Fish. Pathol."},{"key":"10.1016\/j.fsi.2016.01.003_bib8","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1751-0813.2004.tb12650.x","article-title":"Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus","volume":"82","author":"Stephens","year":"2004","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.fsi.2016.01.003_bib9","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3354\/dao069137","article-title":"Detection of the herpesviral hematopoietic necrosis disease agent (Cyprinid herpesvirus 2) in moribund and healthy goldfish: validation of a quantitative PCR diagnostic method","volume":"69","author":"Goodwin","year":"2006","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.fsi.2016.01.003_bib10","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1365-2761.2007.00847.x","article-title":"Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK","volume":"30","author":"Jeffery","year":"2007","journal-title":"J.\u00a0Fish. Dis."},{"key":"10.1016\/j.fsi.2016.01.003_bib11","first-page":"164","article-title":"Mass mortality caused by Cyprinid Herpesvirus 2 (CyHV-2) in Prussian carp (Carassius gibelio) in China","volume":"32","author":"Wang","year":"2012","journal-title":"Bull. Eur. Assoc. Fish. Pathol."},{"key":"10.1016\/j.fsi.2016.01.003_bib12","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetmic.2013.05.025","article-title":"Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China","volume":"166","author":"Xu","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib13","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3354\/dao02535","article-title":"Massive mortality of Prussian carp Carassius gibelio in the upper Elbe basin associated with herpesviral hematopoietic necrosis (CyHV-2)","volume":"102","author":"Danek","year":"2012","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.fsi.2016.01.003_bib14","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.vetmic.2015.04.006","article-title":"Establishment of a novel and highly permissive cell line for the efficient replication of cyprinid herpesvirus 2 (CyHV-2)","volume":"177","author":"Ma","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib15","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.antiviral.2012.06.003","article-title":"Countermeasures against viral diseases of farmed fish","volume":"95","author":"Kibenge","year":"2012","journal-title":"Antivir. Res."},{"key":"10.1016\/j.fsi.2016.01.003_bib16","doi-asserted-by":"crossref","first-page":"899","DOI":"10.2460\/ajvr.75.10.899","article-title":"Efficacy and safety of a modified-live cyprinid herpesvirus 3 vaccine in koi (Cyprinus carpio koi) for prevention of koi herpesvirus disease","volume":"75","author":"Weber","year":"2014","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.fsi.2016.01.003_bib17","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2460\/ajvr.75.10.905","article-title":"Protective immunity of a modified-live cyprinid herpesvirus 3 vaccine in koi (Cyprinus carpio koi) 13 months after vaccination","volume":"75","author":"O'Connor","year":"2014","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.fsi.2016.01.003_bib18","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1097\/00062752-199502060-00014","article-title":"Strategies for viral inactivation","volume":"2","author":"Horowitz","year":"1995","journal-title":"Curr. Opin. Hematol."},{"key":"10.1016\/j.fsi.2016.01.003_bib19","first-page":"73","article-title":"Strategies for viral removal and inactivation","volume":"88","author":"Burstyn","year":"1996","journal-title":"Dev. Biol. Stand"},{"key":"10.1016\/j.fsi.2016.01.003_bib20","first-page":"51","article-title":"Beta-propiolactone and aziridine: their applications in organic synthesis and viral inactivation","volume":"17","author":"Lawrence","year":"1999","journal-title":"Chim. Oggi"},{"key":"10.1016\/j.fsi.2016.01.003_bib21","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/BF00277160","article-title":"Immunogenicity of a viral model vaccine after different inactivation procedures","volume":"183","author":"Bachmann","year":"1994","journal-title":"Med. Microbiol. Immunol."},{"key":"10.1016\/j.fsi.2016.01.003_bib22","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3354\/dao02627","article-title":"Growth of cyprinid herpesvirus 2 (CyHV-2) in cell culture and experimental infection of goldfish Carassius auratus","volume":"105","author":"Ito","year":"2013","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2016.01.003_bib23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetmic.2014.11.005","article-title":"Effect of booster shot and investigation of vaccination efficacy period against herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus","volume":"175","author":"Ito","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib24","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A\u00a0simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib25","first-page":"66","article-title":"Studies on the method of inactivating giant salamander iridovirus with \u03b2-propiolactone","volume":"43","author":"Sun","year":"2013","journal-title":"Freshw. Fish."},{"key":"10.1016\/j.fsi.2016.01.003_bib26","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/j.1095-8649.1973.tb04510.x","article-title":"Routine haematological methods for use with fish blood","volume":"5","author":"Blaxhall","year":"1973","journal-title":"J.\u00a0Fish. Biol."},{"key":"10.1016\/j.fsi.2016.01.003_bib27","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.vetmic.2014.10.028","article-title":"Immunological responses and protection in Chinese giant salamander Andrias davidianus immunized with inactivated iridovirus","volume":"174","author":"Liu","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2016.01.003_bib28","first-page":"275","article-title":"Changes of cell immunity index in blood of southern catfish Silurus meridionalis, immunized by formalin-killed Aeromonas hydrophila","volume":"12","author":"Shan","year":"2005","journal-title":"J.\u00a0Fish. Sci. China"},{"key":"10.1016\/j.fsi.2016.01.003_bib29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jviromet.2007.08.027","article-title":"Comparison of immune responses to different foot-and-mouth disease genetically engineered vaccines in guinea pigs","volume":"147","author":"Yao","year":"2008","journal-title":"J.\u00a0Virol. Methods"},{"key":"10.1016\/j.fsi.2016.01.003_bib30","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u25b3\u25b3Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2016.01.003_bib31","first-page":"447","article-title":"Potency testing of fish vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev. Biol. Stand"},{"key":"10.1016\/j.fsi.2016.01.003_bib32","first-page":"72","article-title":"The discovery and studies of the extensive erythrocytic phagocytosis","volume":"20","author":"Wang","year":"1996","journal-title":"J.\u00a0Fish. Sci. China"},{"key":"10.1016\/j.fsi.2016.01.003_bib33","series-title":"Fish Immunology","first-page":"29","author":"Macarthur","year":"1985"},{"key":"10.1016\/j.fsi.2016.01.003_bib34","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0044-8486(00)00418-X","article-title":"Crustacean haemocytes and haematopoiesis","volume":"191","author":"Johansson","year":"2000","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2016.01.003_bib35","doi-asserted-by":"crossref","first-page":"626","DOI":"10.3724\/SP.J.1231.2010.06655","article-title":"Changes of immune parameters in periphery blood of Spinibarbus sinensis Bleeker immunized with formalin-killed Aeromonas sobria","volume":"34","author":"Luo","year":"2010","journal-title":"J.\u00a0Fish. China"},{"key":"10.1016\/j.fsi.2016.01.003_bib36","first-page":"71","article-title":"Effects of inactivated Nocardia seriolae on the nonspecific immune indexes of Channa argus","volume":"34","author":"Xie","year":"2013","journal-title":"Prog. Fish. Sci."},{"key":"10.1016\/j.fsi.2016.01.003_bib37","first-page":"97","article-title":"Studies on the vaccination against Vibrio alginolyticus in farmed marine Sparus macrocephalus","volume":"29","author":"Wang","year":"2008","journal-title":"J.\u00a0South China Agric. Univ."},{"key":"10.1016\/j.fsi.2016.01.003_bib38","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1182\/blood.V83.8.2023.2023","article-title":"IL-11: a multifunctional growth factor derived from the hematopoietic microenvironment","volume":"83","author":"Du","year":"1994","journal-title":"Blood"},{"key":"10.1016\/j.fsi.2016.01.003_bib39","doi-asserted-by":"crossref","first-page":"3897","DOI":"10.1182\/blood.V89.11.3897","article-title":"Interleukin-11: review of molecular, cell biology, and clinical use","volume":"89","author":"Du","year":"1997","journal-title":"Blood"},{"key":"10.1016\/j.fsi.2016.01.003_bib40","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1165\/rcmb.2002-0044OC","article-title":"Interleukin-11 and interleukin-6 protect cultured human endothelial cells from H2O2\u2212 induced cell death","volume":"29","author":"Waxman","year":"2003","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.fsi.2016.01.003_bib41","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1038\/ni1113","article-title":"The complement system in regulation of adaptive immunity","volume":"5","author":"Carroll","year":"2004","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2016.01.003_bib42","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.imlet.2004.11.010","article-title":"Complement:central to innate immunity and bridging to adaptive responses","volume":"97","author":"Morgan","year":"2005","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.fsi.2016.01.003_bib43","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.fsi.2013.04.028","article-title":"Conserved structural complement component C3 in miiuy croaker Miichthys miiuy and their involvement in pathogenic bacteria induced immunity","volume":"35","author":"Sun","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2016.01.003_bib44","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/j.vaccine.2005.08.087","article-title":"Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a microarray analysis","volume":"24","author":"Byon","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2016.01.003_bib45","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1038\/nri891","article-title":"Antibodies, viruses and vaccines","volume":"2","author":"Burton","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2016.01.003_bib46","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1016\/j.vaccine.2005.01.096","article-title":"Protection of cultured Cyprinus carpio against a lethal viral disease by an attenuated virus vaccine","volume":"23","author":"Perelberg","year":"2005","journal-title":"Vaccine"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464816300031?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464816300031?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T22:09:43Z","timestamp":1572991783000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464816300031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":46,"alternative-id":["S1050464816300031"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2016.01.003","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2016,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immune response and protection in gibel carp, Carassius gibelio, after vaccination with \u03b2-propiolactone inactivated cyprinid herpesvirus 2","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2016.01.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}