{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:30:53Z","timestamp":1785252653341,"version":"3.55.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"National Key Technology R&D Program","award":["2012BAD25B02"],"award-info":[{"award-number":["2012BAD25B02"]}]},{"name":"Science and Technology Planning Project of Jiangxi Province","award":["20152ACF60021"],"award-info":[{"award-number":["20152ACF60021"]}]},{"DOI":"10.13039\/501100005906","name":"Chinese Academy of Fishery Sciences","doi-asserted-by":"crossref","award":["2016ZD0503"],"award-info":[{"award-number":["2016ZD0503"]}],"id":[{"id":"10.13039\/501100005906","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.fsi.2016.04.133","type":"journal-article","created":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T11:50:37Z","timestamp":1462103437000},"page":"473-480","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":64,"special_numbering":"C","title":["Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of grass carp reovirus"],"prefix":"10.1016","volume":"54","author":[{"given":"Weiwei","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchen","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunbin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjian","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoujun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2016.04.133_bib1","first-page":"670437","article-title":"Insights into the antiviral immunity against grass carp (Ctenopharyngodon idella) reovirus (GCRV) in grass carp","volume":"2015","author":"Rao","year":"2015","journal-title":"J.\u00a0Immunol. Res."},{"issue":"5","key":"10.1016\/j.fsi.2016.04.133_bib2","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/j.fsi.2012.09.012","article-title":"Molecular cloning, characterization and expression of cathepsin D from grass carp (Ctenopharyngodon idella)","volume":"33","author":"Dong","year":"2012","journal-title":"Fish Shellfish Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2016.04.133_bib3","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.fsi.2013.05.029","article-title":"Status and future perspectives of vaccines for industrialised fin-fish farming","volume":"35","author":"Brudeseth","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"22","key":"10.1016\/j.fsi.2016.04.133_bib4","doi-asserted-by":"crossref","first-page":"12466","DOI":"10.1128\/JVI.02333-12","article-title":"Complete genome sequence of a reovirus isolated from grass carp, indicating different genotypes of GCRV in China","volume":"86","author":"Wang","year":"2012","journal-title":"J.\u00a0Virol."},{"issue":"3","key":"10.1016\/j.fsi.2016.04.133_bib5","first-page":"315","article-title":"Growth characteristics and high titer culture of grass carp hemorrhage virus (GCHV)-873 in\u00a0vitro","volume":"4","author":"Fang","year":"1989","journal-title":"Virol. Sin."},{"key":"10.1016\/j.fsi.2016.04.133_bib6","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/0959-8030(94)90036-1","article-title":"Viral infections of aquatic animals with special reference to Asian aquaculture","volume":"4","author":"Ahne","year":"1994","journal-title":"Annu. Rev. Fish Dis."},{"issue":"1","key":"10.1016\/j.fsi.2016.04.133_bib7","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jviromet.2010.06.009","article-title":"An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus","volume":"169","author":"Zhang","year":"2010","journal-title":"J.\u00a0Virol. Methods"},{"issue":"3\u20134","key":"10.1016\/j.fsi.2016.04.133_bib8","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.vetmic.2011.07.032","article-title":"Bdellovibrios, potential biocontrol bacteria against pathogenic Aeromonas hydrophila","volume":"154","author":"Cao","year":"2012","journal-title":"Vet. Microbiol."},{"issue":"2","key":"10.1016\/j.fsi.2016.04.133_bib9","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.fsi.2013.04.022","article-title":"Development of a novel candidate subunit vaccine against Grass carp reovirus Guangdong strain (GCRV-GD108)","volume":"35","author":"Tian","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"25","key":"10.1016\/j.fsi.2016.04.133_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13337-013-0186-4","article-title":"Viral vaccines for farmed finfish","volume":"1","author":"Dhar","year":"2014","journal-title":"Virus Dis."},{"issue":"2","key":"10.1016\/j.fsi.2016.04.133_bib11","first-page":"111","article-title":"The preparation and immune effect of attenuated live vaccine obtained through cell culture for hemorrhage of grass carp","volume":"18","author":"Xu","year":"1994","journal-title":"J.\u00a0Fish. Sci. China"},{"issue":"6","key":"10.1016\/j.fsi.2016.04.133_bib13","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1016\/j.fsi.2013.09.031","article-title":"A\u00a0history of fish vaccination: science-based disease prevention in aquaculture","volume":"35","author":"Gudding","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"12","key":"10.1016\/j.fsi.2016.04.133_bib14","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.4014\/jmb.1505.05041","article-title":"Yeast surface display of capsid protein VP7 of grass carp reovirus: fundamental investigation for the development of vaccine against hemorrhagic disease","volume":"25","author":"Luo","year":"2015","journal-title":"J.\u00a0Microbiol. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2016.04.133_bib15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.virusres.2011.10.014","article-title":"Complete genomic sequence of a reovirus isolated from grass carp in China","volume":"163","author":"Ye","year":"2012","journal-title":"Virus Res."},{"issue":"4","key":"10.1016\/j.fsi.2016.04.133_bib16","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s00705-008-0048-3","article-title":"Characterization of infectious particles of grass carp reovirus by treatment with proteases","volume":"153","author":"Fang","year":"2008","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2016.04.133_bib17","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1186\/1743-422X-11-178","article-title":"Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration","volume":"11","author":"Liang","year":"2014","journal-title":"Virol. J."},{"issue":"10","key":"10.1016\/j.fsi.2016.04.133_bib18","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1111\/j.1365-2761.2008.00960.x","article-title":"Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines","volume":"31","author":"Anderson","year":"2008","journal-title":"J.\u00a0Fish Dis."},{"issue":"4","key":"10.1016\/j.fsi.2016.04.133_bib19","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00705-013-1864-7","article-title":"Detection of grass carp reovirus (GCRV) with monoclonal antibodies","volume":"159","author":"Hongli","year":"2014","journal-title":"Arch. Virol."},{"issue":"2","key":"10.1016\/j.fsi.2016.04.133_bib20","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.fsi.2014.11.026","article-title":"Protective immunity of grass carp immunized with DNA vaccine encoding the vp7 gene of grass carp reovirus using carbon nanotubes as a carrier molecule","volume":"42","author":"Zhu","year":"2015","journal-title":"Fish Shellfish Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2016.04.133_bib21","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.imlet.2009.07.001","article-title":"Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice","volume":"125","author":"Li","year":"2009","journal-title":"Immunol. Lett."},{"issue":"4","key":"10.1016\/j.fsi.2016.04.133_bib22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2016.04.133_bib23","first-page":"447","article-title":"Potency testing of fish vaccines. International symposium on fish biologics: serodiagnostics and vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev. Biol. Stand."},{"key":"10.1016\/j.fsi.2016.04.133_bib24","series-title":"FAO Fishery and Aquaculture","year":"2012"},{"issue":"51","key":"10.1016\/j.fsi.2016.04.133_bib25","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1016\/j.vaccine.2014.08.039","article-title":"Efficacy of a formalin-inactivated vaccine against Streptococcus iniae infection in the farmed grouper Epinephelus coioides by intraperitoneal immunization","volume":"32","author":"Huang","year":"2014","journal-title":"Vaccine"},{"issue":"2","key":"10.1016\/j.fsi.2016.04.133_bib26","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.fsi.2014.05.015","article-title":"Immune responses to live and inactivated Nocardia seriolae and protective effect of recombinant interferon gamma (rIFN\u03b3) against nocardiosis in ginbuna crucian carp, Carassius auratus langsdorfii","volume":"39","author":"Nayak","year":"2014","journal-title":"Fish Shellfish Immunol."},{"issue":"35","key":"10.1016\/j.fsi.2016.04.133_bib27","doi-asserted-by":"crossref","first-page":"4261","DOI":"10.1016\/j.vaccine.2015.03.077","article-title":"An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures","volume":"33","author":"Pereira","year":"2015","journal-title":"Vaccine"},{"issue":"6","key":"10.1016\/j.fsi.2016.04.133_bib28","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1586\/erv.12.38","article-title":"Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges","volume":"11","author":"Delrue","year":"2012","journal-title":"Expert Rev. Vaccines"},{"issue":"5","key":"10.1016\/j.fsi.2016.04.133_bib29","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/sj.jim.2900391","article-title":"Production methods for rabies vaccine","volume":"18","author":"Perez","year":"1997","journal-title":"J.\u00a0Ind. Microbiol."},{"issue":"3","key":"10.1016\/j.fsi.2016.04.133_bib30","doi-asserted-by":"crossref","first-page":"291","DOI":"10.2174\/157489106778777673","article-title":"Advances in the development of inactivated virus vaccines","volume":"1","author":"Stauffer","year":"2006","journal-title":"Recent Pat. Antiinfect. Drug Discov."},{"issue":"4","key":"10.1016\/j.fsi.2016.04.133_bib31","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0264-410X(90)90083-X","article-title":"Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine","volume":"8","author":"Bahnemann","year":"1990","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2016.04.133_bib32","first-page":"129","article-title":"Dengue vaccine candidates in development","volume":"338","author":"Durbin","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2016.04.133_bib33","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.molimm.2007.06.356","article-title":"A\u00a0new isotype of immunoglobulin heavy chain in the urodele amphibian Pleurodeles waltl predominantly expressed in larvae","volume":"45","author":"Schaerlinger","year":"2008","journal-title":"Mol. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2016.04.133_bib34","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.fsi.2009.06.009","article-title":"Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)","volume":"27","author":"Su","year":"2009","journal-title":"Fish Shellfish Immunol."},{"issue":"6\u20137","key":"10.1016\/j.fsi.2016.04.133_bib35","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.biochi.2007.04.015","article-title":"Interferon-induced Mx proteins in antiviral host defense","volume":"89","author":"Haller","year":"2007","journal-title":"Biochimie"},{"issue":"3","key":"10.1016\/j.fsi.2016.04.133_bib36","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.fsi.2013.01.003","article-title":"Trunk kidney of grass carp (Ctenopharyngodon idella) mediates immune responses against GCRV and viral\/bacterial PAMPs in\u00a0vivo and in\u00a0vitro","volume":"34","author":"Chen","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2016.04.133_bib37","first-page":"177","article-title":"Inactivated vaccine for hemorrhage of grass carp up-regulates the expressions of major immune-related genes in spleen of grass carp","volume":"31","author":"Yi","year":"2015","journal-title":"Chin. J. Cell. Mol. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464816302844?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464816302844?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T10:57:23Z","timestamp":1536749843000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464816302844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":36,"alternative-id":["S1050464816302844"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2016.04.133","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of grass carp reovirus","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2016.04.133","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}