{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:08:36Z","timestamp":1791137316121,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302208"],"award-info":[{"award-number":["31302208"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science & Technology Program of The General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China","award":["2015IK055"],"award-info":[{"award-number":["2015IK055"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2017,4]]},"DOI":"10.1016\/j.fsi.2017.01.037","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T13:00:21Z","timestamp":1485867621000},"page":"97-102","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":55,"special_numbering":"C","title":["Molecular identification and expression analysis of TLR5M and TLR5S from orange-spotted grouper ( Epinepheluscoioides )"],"prefix":"10.1016","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0694-0699","authenticated-orcid":false,"given":"Jian-Shan","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Qiong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Hua","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang-Zhi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ming","family":"Dan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Chun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.01.037_bib1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.smim.2006.12.004","article-title":"TLR signaling","volume":"19","author":"Kawai","year":"2007","journal-title":"Seminars Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib2","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.bbrc.2009.08.062","article-title":"Toll-like receptors and innate immunity","volume":"388","author":"Kumar","year":"2009","journal-title":"Biochem. Bioph. Res. Co."},{"key":"10.1016\/j.fsi.2017.01.037_bib3","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern recognition receptors and inflammation","volume":"140","author":"Takeuchi","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2017.01.037_bib4","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.cell.2006.02.015","article-title":"Pathogen recognition and innate immunity","volume":"124","author":"Akira","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2017.01.037_bib5","first-page":"5","article-title":"Toll-like receptor signaling","author":"Lim","year":"2013","journal-title":"Csh. Perspect. Biol."},{"key":"10.1016\/j.fsi.2017.01.037_bib6","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.4049\/jimmunol.167.4.1882","article-title":"Bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression","volume":"167","author":"Gewirtz","year":"2001","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib7","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2009.09.021","article-title":"Toll-like receptor signaling in bony fish","volume":"134","author":"Rebl","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2017.01.037_bib8","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fsi.2013.03.374","article-title":"Molecular cloning, phylogenetic analysis and functional characterization of soluble Toll-like receptor 5 in gilthead seabream, Sparus aurata","volume":"35","author":"Mu\u00f1oz","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib9","doi-asserted-by":"crossref","first-page":"48588","DOI":"10.1074\/jbc.M407634200","article-title":"Sensing bacterial flagellin by membrane and soluble orthologs of Toll-like receptor 5 in rainbow trout (Onchorhynchus mikiss)","volume":"279","author":"Tsujita","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.fsi.2017.01.037_bib10","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1016\/j.vaccine.2005.11.003","article-title":"Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin","volume":"24","author":"Tsujita","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.01.037_bib11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.fsi.2015.01.024","article-title":"Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection","volume":"44","author":"Jiang","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib12","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.molimm.2003.10.003","article-title":"Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish","volume":"40","author":"Meijer","year":"2004","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.dci.2014.02.019","article-title":"Identification of IRAK-4 in grouper (Epinephelus coioides) that impairs MyD88-dependent NF-\u03baB activation","volume":"45","author":"Li","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib14","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.dci.2016.03.028","article-title":"Identification and characterization of three TLR1 subfamily members from the orange-spotted grouper, Epinephelus coioides","volume":"61","author":"Li","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib15","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/jfb.12550","article-title":"Orange-spotted grouper Epinephelus coioides Tak1: molecular identification, expression analysis and functional study","volume":"86","author":"Li","year":"2015","journal-title":"J.\u00a0Fish. Biol."},{"key":"10.1016\/j.fsi.2017.01.037_bib16","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.fsi.2006.04.011","article-title":"Protective immunity in grouper (Epinephelus coioides) following exposure to or injection with Cryptocaryon irritans","volume":"22","author":"Luo","year":"2007","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib17","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/1471-2164-8-124","article-title":"Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors","volume":"8","author":"Matsushima","year":"2007","journal-title":"BMC Genomics"},{"key":"10.1016\/j.fsi.2017.01.037_bib18","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u25b3\u25b3Ct Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2017.01.037_bib19","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s00251-013-0694-9","article-title":"Comprehensive survey and genomic characterization of Toll-like receptors (TLRs) in channel catfish, Ictalurus punctatus: identification of novel fish TLRs","volume":"34","author":"Quiniou","year":"2013","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2017.01.037_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-10-235","article-title":"A\u00a0nested leucine rich repeat (LRR) domain: the precursor of LRRs is a ten or eleven residue motif","volume":"10","author":"Matsushima","year":"2010","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.fsi.2017.01.037_bib21","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2017.01.037_bib22","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1073\/pnas.95.2.588","article-title":"A\u00a0family of human receptors structurally related to Drosophila\u2009Toll","volume":"95","author":"Rock","year":"1998","journal-title":"P.\u00a0Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2017.01.037_bib23","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1006\/geno.2000.6115","article-title":"Cloning and characterization of the Murine Toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF\/Ei Mice","volume":"64","author":"Sebastiani","year":"2000","journal-title":"Genomics"},{"key":"10.1016\/j.fsi.2017.01.037_bib24","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.fsi.2011.10.031","article-title":"Inductive expression of toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp, mrigal (Cirrhinus mrigala)","volume":"32","author":"Basu","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib25","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1089\/dna.2013.2051","article-title":"Characterization and expression analysis of common carp Cyprinus carpio TLR5M","volume":"32","author":"Duan","year":"2013","journal-title":"DNA.Cell. Biol."},{"key":"10.1016\/j.fsi.2017.01.037_bib26","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.fsi.2010.06.011","article-title":"Molecular cloning and expression study on Toll-like receptor 5 paralogs in Japanese flounder, Paralichthys olivaceus","volume":"29","author":"Hwang","year":"2010","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib27","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.fsi.2007.06.002","article-title":"Divergent Toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21","volume":"23","author":"Baoprasertkul","year":"2007","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib28","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1016\/j.dci.2011.11.009","article-title":"Mucosal immunoglobulins and B cells of teleost fish","volume":"35","author":"Salinas","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.01.037_bib29","doi-asserted-by":"crossref","first-page":"486","DOI":"10.17221\/3294-VETMED","article-title":"Innate and adaptive immunity in teleost fish: a review","volume":"56","author":"Uribe","year":"2011","journal-title":"Vet. Med-czech"},{"key":"10.1016\/j.fsi.2017.01.037_bib30","first-page":"88","article-title":"Immunology in the liver\u2013from homeostasis to disease. Nature reviews","volume":"13","author":"Heymann","year":"2016","journal-title":"Gastroenterology hepatology"},{"key":"10.1016\/j.fsi.2017.01.037_bib31","first-page":"344","article-title":"Cellular and molecular functions of hepatic stellate cells in inflammatory responses and liver immunology","volume":"3","author":"Weiskirchen","year":"2014","journal-title":"Hepatobiliary Surg. Nutr."},{"key":"10.1016\/j.fsi.2017.01.037_bib32","first-page":"727","article-title":"Fish host range of seven isolates of Cryptocaryon irritans (Ciliophora)","volume":"46","author":"Burgess","year":"1995","journal-title":"J.\u00a0Fish. Biol."},{"key":"10.1016\/j.fsi.2017.01.037_bib33","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1023\/A:1018360323287","article-title":"Cryptocaryon irritans Brown 1951, the cause of \u2018white spot disease\u2019 in marine fish: an update","volume":"1","author":"Colorni","year":"1997","journal-title":"Aquar. Sci. Conserv."},{"key":"10.1016\/j.fsi.2017.01.037_bib34","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1007\/s00436-008-0904-9","article-title":"Some characteristics of host-parasite relationship for Cryptocaryon irritans isolated from South China","volume":"102","author":"Luo","year":"2008","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.01.037_bib35","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.fsi.2010.01.006","article-title":"A\u00a0novel protein isolated from the serum of rabbitfish (Siganus oramin) is lethal to Cryptocaryon irritans","volume":"29","author":"Wang","year":"2010","journal-title":"Fish. Shellfish Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817300554?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817300554?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T00:14:25Z","timestamp":1568765665000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817300554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4]]},"references-count":35,"alternative-id":["S1050464817300554"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.01.037","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular identification and expression analysis of TLR5M and TLR5S from orange-spotted grouper (Epinepheluscoioides)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.01.037","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}