{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:58:39Z","timestamp":1786989519896,"version":"build-2736575974"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572652"],"award-info":[{"award-number":["31572652"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Open Fund of the Key Laboratory of Fishery Drug Development","award":["201504"],"award-info":[{"award-number":["201504"]}]},{"name":"Modern Agricultural Industry and Technology System of Shandong Province","award":["SDAIT-12-06"],"award-info":[{"award-number":["SDAIT-12-06"]}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2013CQ029"],"award-info":[{"award-number":["ZR2013CQ029"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Advanced Talents Foundation of Qingdao Agricultural University","award":["6631114322"],"award-info":[{"award-number":["6631114322"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.fsi.2017.03.001","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T14:31:40Z","timestamp":1488465100000},"page":"104-110","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["TC38, a teleost TFPI-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids"],"prefix":"10.1016","volume":"64","author":[{"given":"Min","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai-hua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-feng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning-qiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.03.001_bib1","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1073\/pnas.92.8.3449","article-title":"Retro and retroenantio analogs of cecropin-melittin hybrids","volume":"92","author":"Merrifield","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2017.03.001_bib2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s00253-008-1829-z","article-title":"Antibiotic resistance genes in water environment","volume":"82","author":"Zhang","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.fsi.2017.03.001_bib3","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1016\/j.watres.2012.02.004","article-title":"Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment","volume":"46","author":"Gao","year":"2012","journal-title":"Water Res."},{"key":"10.1016\/j.fsi.2017.03.001_bib4","first-page":"30","article-title":"Factors influencing multi-drug resistant and plasmid DNA harbouring Aeromonas hydrophila isolated from Lake Manzala, Egypt","volume":"2","author":"Zaky","year":"2010","journal-title":"J.\u00a0Bacteriol. Res."},{"key":"10.1016\/j.fsi.2017.03.001_bib5","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/(SICI)1099-1387(199705)3:3<181::AID-PSC97>3.0.CO;2-K","article-title":"New caerin antibacterial peptides from the skin glands of the Australian tree frog Litoria xanthomera","volume":"3","author":"Steinborner","year":"1997","journal-title":"J.\u00a0Pept. Sci."},{"key":"10.1016\/j.fsi.2017.03.001_bib6","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1006\/bbrc.1996.0071","article-title":"A\u00a0novel antimicrobial peptide from Bufo bufo gargarizans. Biochem","volume":"218","author":"Park","year":"1996","journal-title":"Biophys. Res. Commun."},{"key":"10.1016\/j.fsi.2017.03.001_bib7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0965-1748(97)00013-1","article-title":"Novel antibacterial peptides isolated from a European bumblebee, Bombus pascuorum (Hymenoptera, Apoidea)","volume":"27","author":"Rees","year":"1997","journal-title":"Insect biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2017.03.001_bib8","doi-asserted-by":"crossref","first-page":"28375","DOI":"10.1074\/jbc.271.45.28375","article-title":"Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities","volume":"271","author":"Skerlavaj","year":"1996","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.fsi.2017.03.001_bib9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0014-5793(97)00684-4","article-title":"A\u00a0novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus","volume":"411","author":"Park","year":"1997","journal-title":"FEBS Lett."},{"key":"10.1016\/j.fsi.2017.03.001_bib10","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1432-1033.1997.00743.x","article-title":"Purification, characterisation and cDNA cloning of an antimicrobial peptide from Macadamia integrifolia","volume":"244","author":"Marcus","year":"1997","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.fsi.2017.03.001_bib11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-2672.1997.00241.x","article-title":"Pediocin PD-1, a bactericidal antimicrobial peptide from Pediococcus damnosus NCFB 1832","volume":"83","author":"Green","year":"1997","journal-title":"J.\u00a0Appl. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.001_bib12","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/415389a","article-title":"Antimicrobial peptides of multicellular organisms","volume":"415","author":"Zasloff","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2017.03.001_bib13","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1146\/annurev.mi.49.100195.001425","article-title":"Peptides as weapons against microorganisms in the chemical defense system of vertebrates","volume":"49","author":"Nicolas","year":"1995","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.001_bib14","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1146\/annurev.iy.13.040195.000425","article-title":"Peptide antibiotics and their role in innate immunity","volume":"13","author":"Boman","year":"1995","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib15","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1128\/AAC.01619-12","article-title":"Two hits are better than one: membrane-active and DNA binding-related double-action mechanism of NK-18, a novel antimicrobial peptide derived from mammalian NK-lysin, Antimicrob","volume":"57","author":"Yan","year":"2013","journal-title":"Agents Chemother."},{"key":"10.1016\/j.fsi.2017.03.001_bib16","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/nar\/gkh025","article-title":"APD: the antimicrobial peptide database","volume":"32","author":"Wang","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2017.03.001_bib17","doi-asserted-by":"crossref","first-page":"11452","DOI":"10.1021\/bi9630826","article-title":"The concentration-dependent membrane activity of cecropin A","volume":"36","author":"Silvestro","year":"1997","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2017.03.001_bib18","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/292246a0","article-title":"Sequence and specificity of two antibacterial proteins involved in insect immunity","volume":"292","author":"Steiner","year":"1991","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2017.03.001_bib19","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1002\/pro.5560030902","article-title":"Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores","volume":"3","author":"Wimley","year":"1997","journal-title":"Protein Sci."},{"key":"10.1016\/j.fsi.2017.03.001_bib20","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0005-2728(89)80344-5","article-title":"Interactions between a new class of eukaryotic antimicrobial agents and isolated rat liver mitochondria","volume":"975","author":"Westerhoff","year":"1989","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.fsi.2017.03.001_bib21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0005-2736(97)00051-5","article-title":"Interactions of an antimicrobial peptide, magainin 2, with outer and inner membranes of Gram-negative bacteria","volume":"1327","author":"Matsuzaki","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.fsi.2017.03.001_bib22","doi-asserted-by":"crossref","first-page":"e106543","DOI":"10.1371\/journal.pone.0106543","article-title":"NKLP27: a Teleost NK-Lysin Peptide that modulates immune response, induces degradation of bacterial DNA, and inhibits bacterial and viral infection","volume":"9","author":"Zhang","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2017.03.001_bib23","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1006\/bbrc.1998.8159","article-title":"Mechanism of action of the antimicrobial peptide buforin II: buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions","volume":"244","author":"Park","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fsi.2017.03.001_bib24","first-page":"200","article-title":"The intracellular mechanism of action on Escherichia coli of BF2-A\/C, two analogues of the antimicrobial peptide Buforin 2","volume":"51","author":"Hao","year":"2013","journal-title":"J.\u00a0Microbiol."},{"key":"10.1016\/j.fsi.2017.03.001_bib25","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1073\/pnas.91.8.3353","article-title":"Molecular cloning, expression, and partial characterization of a second human tissue-factor pathway inhibitor","volume":"91","author":"Sprecher","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2017.03.001_bib26","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1093\/oxfordjournals.jbchem.a124648","article-title":"cDNA cloning and mRNA expression of a serine proteinase inhibitor secreted by cancer cells: identification as placental protein 5 and tissue factor pathway inhibitor-2","volume":"116","author":"Miyagi","year":"1994","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.fsi.2017.03.001_bib27","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1160\/TH05-07-0509","article-title":"Structure, function and biology of tissue factor pathway inhibitor-2","volume":"94","author":"Chand","year":"2005","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.fsi.2017.03.001_bib28","doi-asserted-by":"crossref","first-page":"e1003803","DOI":"10.1371\/journal.ppat.1003803","article-title":"The TFPI-2 derived peptide EDC34 improves outcome of gram-negative sepsis","volume":"9","author":"Papareddy","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2017.03.001_bib29","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12866-016-0750-3","article-title":"Vertebrate TFPI-2 C-terminal peptides exert therapeutic applications against Gram-negative infections","volume":"16","author":"Kasetty","year":"2016","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.fsi.2017.03.001_bib30","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.fsi.2016.10.003","article-title":"Molecular characterization, expression analysis, and bactericidal activity of the derivative peptides of TFPI-1 and TFPI-2 in half-smooth tongue sole, Cynoglossus semilaevis","volume":"58","author":"Zhao","year":"2016","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib31","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1099\/mic.0.2008\/017343-0","article-title":"Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain","volume":"154","author":"Zhang","year":"2008","journal-title":"Microbiology"},{"key":"10.1016\/j.fsi.2017.03.001_bib32","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1111\/j.1365-2109.2011.02861.x","article-title":"Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China","volume":"43","author":"Zhang","year":"2012","journal-title":"Aquac. Res."},{"key":"10.1016\/j.fsi.2017.03.001_bib33","doi-asserted-by":"crossref","first-page":"4047","DOI":"10.1016\/j.vaccine.2009.04.023","article-title":"Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross protective vaccine","volume":"27","author":"Wang","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.001_bib34","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/jfb.12540","article-title":"A\u00a0novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi: development and characterization","volume":"86","author":"Fu","year":"2015","journal-title":"J.\u00a0Fish. Biol."},{"key":"10.1016\/j.fsi.2017.03.001_bib35","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.fsi.2012.09.010","article-title":"Construction and analysis of experimental DNA vaccines against megalocytivirus","volume":"33","author":"Zhang","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib36","article-title":"A\u00a0TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole","author":"He","year":"2016","journal-title":"Cynoglossus Semilaevis. Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib37","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1186\/s40064-016-2760-x","article-title":"Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated with fish disease","volume":"5","author":"Zhou","year":"2016","journal-title":"Springerplus"},{"key":"10.1016\/j.fsi.2017.03.001_bib38","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.fsi.2016.11.054","article-title":"Fatty acid synthase plays a positive role in shrimp immune responses against Vibrio parahaemolyticus infection","volume":"60","author":"Zuo","year":"2016","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib39","doi-asserted-by":"crossref","first-page":"e0165500","DOI":"10.1371\/journal.pone.0165500","article-title":"Vibrio vulnificus type 6 secretion system 1 contains anti-bacterial properties","volume":"11","author":"Church","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2017.03.001_bib40","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.fsi.2014.10.008","article-title":"Protein encoded by ORF 093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi","volume":"42","author":"Li","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.001_bib41","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nrmicro1098","article-title":"Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?","volume":"3","author":"Brogden","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.001_bib42","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1016\/j.bbamem.2006.02.001","article-title":"Molecular mechanism of antimicrobial peptides: the origin of cooperativity","volume":"1758","author":"Huang","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.fsi.2017.03.001_bib43","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jcis.2007.02.070","article-title":"Effect of lipid head group composition on the interaction between melittin and lipid bilayers","volume":"311","author":"Stromstedt","year":"2007","journal-title":"J.\u00a0Colloid Interface Sci."},{"key":"10.1016\/j.fsi.2017.03.001_bib44","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1529\/biophysj.105.073890","article-title":"Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin","volume":"91","author":"Ramamoorthy","year":"2006","journal-title":"Biophys. J."},{"key":"10.1016\/j.fsi.2017.03.001_bib45","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S0196-9781(03)00172-4","article-title":"In\u00a0vitro and in\u00a0vivo activity of an antibacterial peptide analog against uropathogens","volume":"24","author":"Cudic","year":"2003","journal-title":"Peptides"},{"key":"10.1016\/j.fsi.2017.03.001_bib46","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/35086601","article-title":"Antibacterial agents based on the cyclic D, L-\u03b1-peptide architecture","volume":"412","author":"Fernandez-Lopez","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2017.03.001_bib47","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.3892\/ijmm.2013.1291","article-title":"Activity of antibacterial protein from maggots against Staphylococcus aureus in\u00a0vitro and in\u00a0vivo","volume":"31","author":"Zhang","year":"2013","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.fsi.2017.03.001_bib48","doi-asserted-by":"crossref","first-page":"e40135","DOI":"10.1371\/journal.pone.0040135","article-title":"Antibacterial activity and mechanism of a scorpion venom peptide derivative in\u00a0vitro and in\u00a0vivo","volume":"7","author":"Cao","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2017.03.001_bib49","first-page":"1860","article-title":"Effects of antimicrobial peptides supplementation on growth, nonspecific immunity and disease resistance of Koi (Cyprinus carpio koi)","volume":"25","author":"Lin","year":"2013","journal-title":"Chin. J. Anim. Nutr."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301201?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301201?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,9,19]],"date-time":"2019-09-19T03:39:23Z","timestamp":1568864363000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817301201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":49,"alternative-id":["S1050464817301201"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.001","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"TC38, a teleost TFPI-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}