{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T02:42:39Z","timestamp":1772678559429,"version":"3.50.1"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Natural Science Foundation of China and Henan Province","award":["U1604104"],"award-info":[{"award-number":["U1604104"]}]},{"name":"Program for Innovative Research Team (in Science and Technology) in University of Henan Province","award":["15IRTSTHN018"],"award-info":[{"award-number":["15IRTSTHN018"]}]},{"name":"Key Scientific Research Project in University of Henan Province","award":["17B240004"],"award-info":[{"award-number":["17B240004"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.fsi.2017.03.004","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T23:21:48Z","timestamp":1488496908000},"page":"1-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Molecular characterization and expression analysis of toll-like receptors 5 and 22 from natural triploid Carassius auratus"],"prefix":"10.1016","volume":"64","author":[{"given":"Jie","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghui","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianliang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.03.004_bib1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.imlet.2013.11.013","article-title":"Toll-like receptors (TLRs) in aquatic animals: signaling pathways, expressions and immune responses","volume":"158","author":"Rauta","year":"2014","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.fsi.2017.03.004_bib2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.dci.2012.04.001","article-title":"Advances in research of fish immune-relevant genes: a comparative overview of innate and adaptive immunity in teleosts","volume":"39","author":"Zhu","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib3","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.fsi.2014.09.022","article-title":"Toll-like receptor recognition of bacteria in fish: ligand specificity and signal pathways","volume":"41","author":"Zhang","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib4","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1002\/jez.b.22559","article-title":"A\u00a0tetrapod-like repertoire of innate immune receptors and effectors for coelacanths","volume":"322B","author":"Boudinot","year":"2014","journal-title":"J.\u00a0Exp. Zool. Mol. Dev. Evol.)"},{"key":"10.1016\/j.fsi.2017.03.004_bib5","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.fsi.2015.06.017","article-title":"Structural characterization and evolutionary analysis of fish-specific TLR27","volume":"45","author":"Wang","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib6","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern recognition receptors and inflammation","volume":"140","author":"Takeuchi","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2017.03.004_bib7","doi-asserted-by":"crossref","first-page":"26705","DOI":"10.1038\/srep26705","article-title":"Ectodomain architecture affects sequence and functional evolution of vertebrate toll-like receptors","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2017.03.004_bib8","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2017.03.004_bib9","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1038\/ni1011","article-title":"Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility","volume":"4","author":"Smith","year":"2003","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.dci.2013.08.010","article-title":"Ligand specificities of Toll-like receptors in fish: indications from infection studies","volume":"43","author":"Pietretti","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib11","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.fsi.2011.10.031","article-title":"Inductive expression of toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp, mrigal (Cirrhinus mrigala)","volume":"32","author":"Basu","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib12","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1007\/s00251-002-0519-8","article-title":"Prediction of the prototype of the human Toll-like receptor gene family from the pufferfish, Fugu rubripes, genome","volume":"54","author":"Oshiumi","year":"2003","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2017.03.004_bib13","doi-asserted-by":"crossref","first-page":"48588","DOI":"10.1074\/jbc.M407634200","article-title":"Sensing bacterial flagellin by membrane and soluble orthologs of Toll-like receptor 5 in rainbow trout (Onchorhynchus mikiss)","volume":"279","author":"Tsujita","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.fsi.2017.03.004_bib14","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.fsi.2010.06.011","article-title":"Molecular cloning and expression study on Toll-like receptor 5 paralogs in Japanese flounder, Paralichthys olivaceus","volume":"29","author":"Hwang","year":"2010","journal-title":"Fish. Shell\ufb01sh Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib15","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.molimm.2010.12.010","article-title":"TLR agonists extend the functional lifespan of professional phagocytic granulocytes in the bony fish gilthead seabream and direct precursor differentiation towards the production of granulocytes","volume":"48","author":"Sepulcre","year":"2011","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib16","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s00251-013-0694-9","article-title":"Comprehensive survey and genomic characterization of Toll-like receptors (TLRs) in channel catfish, Ictalurus punctatus: identification of novel fish TLRs","volume":"65","author":"Quiniou","year":"2013","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2017.03.004_bib17","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.molimm.2006.03.001","article-title":"Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection: striking similarities and obvious differences with mammals","volume":"44","author":"Lin","year":"2007","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib18","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1089\/dna.2013.2051","article-title":"Characterization and expression analysis of common carp Cyprinus carpio TLR5M","volume":"32","author":"Duan","year":"2013","journal-title":"DNA Cell Biol."},{"key":"10.1016\/j.fsi.2017.03.004_bib19","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.fsi.2015.06.023","article-title":"Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii","volume":"46","author":"Tong","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib20","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.fsi.2015.01.024","article-title":"Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection","volume":"44","author":"Jiang","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib21","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.4049\/jimmunol.181.5.3474","article-title":"Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses","volume":"181","author":"Matsuo","year":"2008","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib22","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.dci.2011.03.006","article-title":"Toll-like receptors in bony fish: from genomics to function","volume":"35","author":"Palti","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib23","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.fsi.2012.05.034","article-title":"Orange-spotted grouper (Epinephelus coioides) toll-like receptor 22: molecular characterization, expression pattern and pertinent signaling pathways","volume":"33","author":"Ding","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.dci.2013.12.003","article-title":"Toll-like receptor 22 of gilthead seabream, Sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation","volume":"44","author":"Mu\u00f1oz","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib25","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.fsi.2015.03.001","article-title":"Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus","volume":"44","author":"Hu","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib26","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00251-007-0193-y","article-title":"Phylogenetic and expression analysis of amphibian Xenopus Toll-like receptors","volume":"59","author":"Ishii","year":"2007","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2017.03.004_bib27","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0145-305X(03)00041-7","article-title":"A\u00a0toll-like receptor (TLR) gene that is up-regulated in activated goldfish macrophages","volume":"27","author":"Stafford","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib28","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.molimm.2003.10.001","article-title":"Toll-like receptor gene family and TIR-domain adapters in Danio rerio","volume":"40","author":"Jault","year":"2004","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib29","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s12010-014-1058-0","article-title":"Toll-like receptor 22 in Labeo rohita: molecular cloning, characterization, 3D modeling, and expression analysis following ligands stimulation and bacterial infection","volume":"174","author":"Samanta","year":"2014","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.fsi.2017.03.004_bib30","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.dci.2014.06.016","article-title":"First evidence of comparative responses of Toll-like receptor 22 (TLR22) to relatively resistant and susceptible Indian farmed carps to Argulus siamensis infection","volume":"47","author":"Panda","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib31","first-page":"605","article-title":"The complete mitogenome of natural triploid Carassius auratus in Qihe River, Mitochondr","volume":"27","author":"Zhou","year":"2016","journal-title":"DNA Part A"},{"key":"10.1016\/j.fsi.2017.03.004_bib32","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.fsi.2014.03.001","article-title":"Different expression profiles of Interleukin 11 (IL-11), Intelectin (ITLN) and Purine nucleoside phosphorylase 5a (PNP 5a) in crucian carp (Carassius auratus gibelio) in response to Cyprinid herpesvirus 2 and Aeromonas hydrophila","volume":"38","author":"Podok","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib33","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/jfd.12214","article-title":"Skin immune response in the zebrafish, Danio rerio (Hamilton), to Aeromonas hydrophila infection: a transcriptional profiling approach","volume":"38","author":"L\u00fc","year":"2015","journal-title":"J.\u00a0Fish. Dis."},{"key":"10.1016\/j.fsi.2017.03.004_bib34","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1007\/s11033-014-3033-9","article-title":"Characterization of immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model","volume":"41","author":"Yadav","year":"2014","journal-title":"Mol. Boil. Rep."},{"key":"10.1016\/j.fsi.2017.03.004_bib35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vetimm.2015.08.009","article-title":"Expression patterns of Toll-like receptors in natural triploid Carassius auratus after infection with Aeromonas hydrophila","volume":"168","author":"Zhang","year":"2015","journal-title":"Vet. Immunol. Immunop"},{"key":"10.1016\/j.fsi.2017.03.004_bib36","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.fsi.2013.07.027","article-title":"Molecular characterization and expression analysis of Toll-like receptor 21 cDNA from Paralichthys olivaceus","volume":"35","author":"Gao","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib37","series-title":"Molecular Cloning","author":"Sambrook","year":"2006"},{"key":"10.1016\/j.fsi.2017.03.004_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-8-124","article-title":"Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors","volume":"8","author":"Matsushima","year":"2007","journal-title":"BMC genomics"},{"key":"10.1016\/j.fsi.2017.03.004_bib39","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2017.03.004_bib40","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/S1471-4906(03)00242-4","article-title":"Leucine-rich repeats and pathogen recognition in Toll-like receptors","volume":"24","author":"Bell","year":"2003","journal-title":"Trends Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib41","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1074\/jbc.275.7.4670","article-title":"Identification of two major sites in the type I interleukin-1 receptor cytoplasmic region responsible for coupling to pro-inflammatory signaling pathways","volume":"275","author":"Slack","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.fsi.2017.03.004_bib42","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.fsi.2011.02.014","article-title":"Ontogeny and tissue-specific expression of innate immune related genes in rohu, Labeo rohita (Hamilton)","volume":"30","author":"Nayak","year":"2011","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib43","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.coi.2010.01.006","article-title":"Host-microbe interactions in the developing zebrafish","volume":"22","author":"Kanther","year":"2010","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib44","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.fsi.2009.02.005","article-title":"Isolation and characterization of Toll-like receptor 9 in half-smooth tongue sole Cynoglossus semilaevis","volume":"26","author":"Yu","year":"2009","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib45","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/j.fsi.2012.02.024","article-title":"Cloning and characterization of the grass carp (Ctenopharyngodon idella) Toll-like receptor 22 gene, a fish-specific gene","volume":"32","author":"Lv","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib46","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.fsi.2010.10.014","article-title":"Molecular characterization of a toll-like receptor 22 homologue in large yellow croaker (Pseudosciaena crocea) and promoter activity analysis of its 5\u2019-flanking sequence","volume":"30","author":"Xiao","year":"2011","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib47","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.dci.2011.08.015","article-title":"Identification, mRNA expression and genomic structure of TLR22 and its association with GCRV susceptibility\/resistance in grass carp (Ctenopharyngodon idella)","volume":"36","author":"Su","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib48","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.fsi.2016.07.012","article-title":"Molecular cloning, characterization and expression analysis of Toll-like receptor 5M gene in Japanese sea perch (Lateolabrax japonicas) after bacterial infection","volume":"56","author":"Wang","year":"2016","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib49","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.dci.2016.05.019","article-title":"Characterization of Toll-like receptor gene expression in goldfish (Carassius auratus) during Dactylogyrus intermedius infection","volume":"56","author":"Tu","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.004_bib50","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1126\/science.1215584","article-title":"Structural basis of TLR5-flagellin recognition and signaling","volume":"335","author":"Yoon","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.fsi.2017.03.004_bib51","first-page":"1","article-title":"MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella","volume":"6","author":"Xu","year":"2016","journal-title":"Sci. Rep."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301237?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301237?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T09:16:06Z","timestamp":1601716566000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817301237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":51,"alternative-id":["S1050464817301237"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.004","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization and expression analysis of toll-like receptors 5 and 22 from natural triploid Carassius auratus","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}