{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:21:32Z","timestamp":1785864092518,"version":"3.56.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016T90956"],"award-info":[{"award-number":["2016T90956"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Shaanxi Province, China","award":["2016JQ3016"],"award-info":[{"award-number":["2016JQ3016"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.fsi.2017.03.021","type":"journal-article","created":{"date-parts":[[2017,3,12]],"date-time":"2017-03-12T09:30:49Z","timestamp":1489311049000},"page":"414-425","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"special_numbering":"C","title":["Protective immunity of grass carp induced by DNA vaccine encoding capsid protein gene (vp7) of grass carp reovirus using bacterial ghost as delivery vehicles"],"prefix":"10.1016","volume":"64","author":[{"given":"Kai","family":"Hao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Zhou","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Bo","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"En-Qi","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao-Xue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.03.021_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/670437","article-title":"Insights into the antiviral immunity against grass carp (Ctenopharyngodon idella) reovirus (GCRV) in grass carp","volume":"2015","author":"Rao","year":"2015","journal-title":"J.\u00a0Immunol. Res."},{"key":"10.1016\/j.fsi.2017.03.021_bib2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.fsi.2014.11.026","article-title":"Protective immunity of grass carp immunized with DNA vaccine encoding the vp7 gene of grass carp reovirus using carbon nanotubes as a carrier molecule","volume":"42","author":"Zhu","year":"2015","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib3","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.fsi.2014.09.014","article-title":"Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus","volume":"41","author":"Zhu","year":"2014","journal-title":"Fish. shellfish immuno"},{"key":"10.1016\/j.fsi.2017.03.021_bib4","doi-asserted-by":"crossref","first-page":"6858","DOI":"10.4049\/jimmunol.172.11.6858","article-title":"Bacterial ghosts are an efficient delivery system for DNA vaccines","volume":"172","author":"Ebensen","year":"2004","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib5","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s11259-008-9040-3","article-title":"DNA vaccines and their applications in veterinary practice: current perspectives","volume":"32","author":"Dhama","year":"2008","journal-title":"Vet. Res. Commun."},{"key":"10.1016\/j.fsi.2017.03.021_bib6","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.bioelechem.2006.10.003","article-title":"A\u00a0single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep","volume":"70","author":"Babiuk","year":"2007","journal-title":"Bioelectrochemistry"},{"key":"10.1016\/j.fsi.2017.03.021_bib7","first-page":"114","article-title":"Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus","volume":"5","author":"Anderson","year":"1996","journal-title":"Mol. Mar. Biol. Biotechnol."},{"key":"10.1016\/j.fsi.2017.03.021_bib8","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1006\/fsim.1997.0134","article-title":"Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination","volume":"8","author":"Lorenzen","year":"1998","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib9","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1016\/j.vaccine.2009.04.012","article-title":"The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature","volume":"27","author":"Lorenzen","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.021_bib10","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3354\/dao060011","article-title":"Protection of Atlantic salmon Salmo salar against infectious pancreatic necrosis after DNA vaccination","volume":"60","author":"Mikalsen","year":"2004","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.fsi.2017.03.021_bib11","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1016\/j.vaccine.2008.08.071","article-title":"DNA vaccine protects ornamental koi (Cyprinus carpio koi) against North American spring viremia of carp virus","volume":"26","author":"Emmenegger","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.021_bib12","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1007\/s10126-004-5129-z","article-title":"Analysis of DNA-vaccinated fish reveals viral antigen in muscle, kidney and thymus, and transient histopathologic changes","volume":"7","author":"Garver","year":"2005","journal-title":"Mar. Biotechmol"},{"key":"10.1016\/j.fsi.2017.03.021_bib13","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.fsi.2015.10.029","article-title":"The protective immunity against grass carp reovirus in grass carp induced by a DNA vaccination using single-walled carbon nanotubes as delivery vehicles","volume":"47","author":"Wang","year":"2015","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib14","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0168-3659(02)00267-5","article-title":"Bacterial ghosts as vaccine candidates for veterinary applications","volume":"85","author":"Jalava","year":"2002","journal-title":"J.\u00a0Control. Release"},{"key":"10.1016\/j.fsi.2017.03.021_bib15","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ijmm.2004.04.003","article-title":"Recombinant bacterial ghosts: versatile targeting vehicles and promising vaccine candidates","volume":"294","author":"Hoffelner","year":"2004","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.021_bib16","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1016\/j.addr.2005.01.027","article-title":"Bacterial ghosts as antigen delivery vehicles","volume":"57","author":"Mayr","year":"2005","journal-title":"Adv. Drug. Deliv. Rev."},{"key":"10.1016\/j.fsi.2017.03.021_bib17","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.ijantimicag.2011.01.007","article-title":"Analogues of peptide SMAP-29 with comparable antimicrobial potency and reduced cytotoxicity","volume":"37","author":"Dawson","year":"2011","journal-title":"Int. J. Antimicrob. Ag."},{"key":"10.1016\/j.fsi.2017.03.021_bib18","doi-asserted-by":"crossref","first-page":"6106","DOI":"10.1128\/AEM.69.10.6106-6113.2003","article-title":"Escherichia coli ghost production by expression of lysis gene E and staphylococcal nuclease","volume":"69","author":"Haidinger","year":"2003","journal-title":"Appl. Environ. Microb."},{"key":"10.1016\/j.fsi.2017.03.021_bib19","doi-asserted-by":"crossref","first-page":"e52142","DOI":"10.1371\/journal.pone.0052142","article-title":"Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus","volume":"7","author":"Peng","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.fsi.2017.03.021_bib20","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ymthe.2004.09.024","article-title":"DNA-loaded bacterial ghosts efficiently mediate reporter gene transfer and expression in macrophages","volume":"11","author":"Paukner","year":"2005","journal-title":"Mol. Ther."},{"key":"10.1016\/j.fsi.2017.03.021_bib21","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12033-010-9277-2","article-title":"Novel expression system for combined vaccine production in Edwardsiella tarda ghost and cadaver cells","volume":"46","author":"Choi","year":"2010","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.fsi.2017.03.021_bib22","doi-asserted-by":"crossref","first-page":"6054","DOI":"10.1016\/j.vaccine.2014.09.014","article-title":"Helicobacter pylori outer inflammatory protein DNA vaccine-loaded bacterial ghost enhances immune protective efficacy in C57BL\/6 mice","volume":"32","author":"Chen","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.021_bib23","first-page":"447","article-title":"Potency testing of fish vaccines","volume":"49","author":"Amend","year":"1980","journal-title":"Dev. Biol. Stand."},{"key":"10.1016\/j.fsi.2017.03.021_bib24","first-page":"1","article-title":"Characterization of grass carp reovirus minor core protein VP4","volume":"9","author":"Yan","year":"2012","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.fsi.2017.03.021_bib25","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.dci.2013.01.014","article-title":"Gene expression profiling in live attenuated Edwardsiella tarda vaccine immunized and challenged zebrafish: insights into the basic mechanisms of protection seen in immunized fish","volume":"40","author":"Yang","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.aquaculture.2014.03.016","article-title":"Effects of dietary administration of Shewanella haliotis D4, Bacillus cereus D7 and Aeromonas bivalvium D15, single or combined, on the growth, innate immunity and disease resistance of shrimp, Litopenaeus vannamei","volume":"428","author":"Hao","year":"2014","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2017.03.021_bib27","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2- \u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2017.03.021_bib28","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1586\/erv.11.149","article-title":"Bacterial ghosts as carriers of protein subunit and DNA-encoded antigens for vaccine applications","volume":"11","author":"Muhammad","year":"2014","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.fsi.2017.03.021_bib29","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.fsi.2005.08.005","article-title":"Protection of tilapia (Oreochromis mosambicus) from edwardsiellosis by vaccination with Edwardsiella tarda ghosts","volume":"20","author":"Kwon","year":"2006","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib30","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1128\/IAI.01219-10","article-title":"Iron-regulated lysis of recombinant Escherichia coli in host releases protective antigen and confers biological containment","volume":"79","author":"Guan","year":"2011","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2017.03.021_bib31","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.fsi.2013.06.012","article-title":"Alginate\u2013chitosan\u2013PLGA composite microspheres induce both innate and adaptive immune response through parenteral immunization in fish","volume":"35","author":"Behera","year":"2013","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib32","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.fsi.2010.09.016","article-title":"Poly(lactic-co-glycolic acid) nanoparticles as candidate DNA vaccine carrier for oral immunization of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus","volume":"30","author":"Tian","year":"2011","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib33","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cbi.2008.11.014","article-title":"Changes of lipid peroxidation in carbon disulfide-treated rat nerve tissues and serum","volume":"179","author":"Sun","year":"2009","journal-title":"Chem-biol. Interac"},{"key":"10.1016\/j.fsi.2017.03.021_bib34","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.fsi.2015.09.013","article-title":"Stress, antioxidant defence and mucosal immune responses of the large yellow croaker Pseudosciaena crocea challenged with Cryptocaryon irritans","volume":"47","author":"Yin","year":"2015","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib35","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.fsi.2007.03.013","article-title":"Molecular cloning, differential expression and 3D structural analysis of the MHC class-II \u03b2 chain from sea bass (Dicentrarchus labrax L.)","volume":"23","author":"Buonocore","year":"2007","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib36","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1016\/j.vaccine.2009.06.071","article-title":"Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens","volume":"27","author":"Jiao","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.021_bib37","doi-asserted-by":"crossref","first-page":"4810","DOI":"10.1128\/IAI.73.8.4810-4817.2005","article-title":"Bacterial ghosts as an oral vaccine: a single dose of Escherichia coli O157:H7 bacterial ghosts protects mice against lethal challenge","volume":"73","author":"Mayr","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2017.03.021_bib38","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1146\/annurev.cb.09.110193.001533","article-title":"Tumor necrosis factor, other cytokines and disease","volume":"9","author":"Tracey","year":"1993","journal-title":"Annu. Rev. Cell. Dev. Bi"},{"key":"10.1016\/j.fsi.2017.03.021_bib39","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.dci.2007.09.009","article-title":"Characterization and functional analysis of goldfish (Carassius auratus L.) tumor necrosis factor-alpha","volume":"32","author":"Grayfer","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib40","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.smim.2013.10.018","article-title":"Interleukin-1beta in innate inflammation, autophagy and immunity","volume":"25","author":"Joosten","year":"2013","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib41","doi-asserted-by":"crossref","first-page":"5733","DOI":"10.1016\/j.vaccine.2012.07.008","article-title":"Salmonella typhi Ty21a bacterial ghost vector augments HIV-1 gp140 DNA vaccine-induced peripheral and mucosal antibody responses via TLR4 pathway","volume":"30","author":"Wen","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.021_bib42","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.fsi.2016.09.003","article-title":"Identification and functional characterization of tumor necrosis factor receptor 1 (TNFR1) of grass carp (Ctenopharyngodon idella)","volume":"58","author":"Zhang","year":"2016","journal-title":"Fish. shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.021_bib43","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0168-3659(02)00267-5","article-title":"Bacterial ghosts as vaccine candidates for veterinary applications","volume":"85","author":"Jalava","year":"2002","journal-title":"J.\u00a0Control. Release"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301407?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301407?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T16:01:05Z","timestamp":1535990465000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817301407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":43,"alternative-id":["S1050464817301407"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.021","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protective immunity of grass carp induced by DNA vaccine encoding capsid protein gene (vp7) of grass carp reovirus using bacterial ghost as delivery vehicles","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}