{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:10:26Z","timestamp":1788567026346,"version":"build-2803163510"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Science and Technology Planning","award":["2013B010404010"],"award-info":[{"award-number":["2013B010404010"]}]},{"name":"Science and Technology Planning","award":["2014B020203001"],"award-info":[{"award-number":["2014B020203001"]}]},{"name":"Science and Technology Planning","award":["2016A050502008"],"award-info":[{"award-number":["2016A050502008"]}]},{"name":"National Key Basic Research and Development Project of China","award":["2010CB530000"],"award-info":[{"award-number":["2010CB530000"]}]},{"name":"South Wisdom Valley, Shunde, Guangdong province","award":["2013CXTD03"],"award-info":[{"award-number":["2013CXTD03"]}]},{"name":"Basic Scientific Research Service Fee in Colleges and Universities","award":["16ykpy06"],"award-info":[{"award-number":["16ykpy06"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.fsi.2017.03.030","type":"journal-article","created":{"date-parts":[[2017,3,18]],"date-time":"2017-03-18T13:16:40Z","timestamp":1489843000000},"page":"287-296","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":47,"special_numbering":"C","title":["Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp ( Ctenopharyngodon idellus ): Induces immune responses and has no damage on liver and intestine function"],"prefix":"10.1016","volume":"64","author":[{"given":"Zeli","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengchang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TingJin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongye","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunbin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houjun","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ouqin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengli","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuerong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinbing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.03.030_bib1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1473-3099(04)01252-6","article-title":"Clonorchiasis: a key foodborne zoonosis in China","volume":"5","author":"Lun","year":"2005","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fsi.2017.03.030_bib2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s40249-016-0166-1","article-title":"Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control","volume":"5","author":"Tang","year":"2016","journal-title":"Infect. Dis. Poverty"},{"key":"10.1016\/j.fsi.2017.03.030_bib3","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/S0140-6736(15)60313-0","article-title":"Clonorchiasis","volume":"387","author":"Qian","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.fsi.2017.03.030_bib4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.parint.2011.06.007","article-title":"Clonorchis sinensis and clonorchiasis, an update","volume":"61","author":"Hong","year":"2012","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.fsi.2017.03.030_bib5","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/2049-9957-2-4","article-title":"Time to tackle clonorchiasis in China","volume":"2","author":"Qian","year":"2013","journal-title":"Infect. Dis. Poverty"},{"key":"10.1016\/j.fsi.2017.03.030_bib6","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S1473-3099(11)70294-8","article-title":"Global burden of human food-borne trematodiasis: a systematic review and meta-analysis","volume":"12","author":"F\u00fcrst","year":"2012","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fsi.2017.03.030_bib7","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.3201\/eid1110.050614","article-title":"Emerging foodborne trematodiasis","volume":"11","author":"Keiser","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.fsi.2017.03.030_bib8","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.ijpara.2013.07.007","article-title":"The zoonotic, fish-borne liver flukes Clonorchis sinensis, Opisthorchis felineus and Opisthorchis viverrini","volume":"43","author":"Petney","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.fsi.2017.03.030_bib9","doi-asserted-by":"crossref","first-page":"S103","DOI":"10.3347\/kjp.2009.47.S.S103","article-title":"Fish-borne zoonotic trematode metacercariae in the Republic of Korea","volume":"47","author":"Sohn","year":"2009","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.fsi.2017.03.030_bib10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1586\/14760584.4.1.89","article-title":"Vaccines for fish in aquaculture","volume":"4","author":"Sommerset","year":"2005","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.fsi.2017.03.030_bib11","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.dci.2011.03.007","article-title":"Advances in fish vaccine delivery","volume":"35","author":"Plant","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib12","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1615\/CritRevTherDrugCarrierSyst.2014010102","article-title":"Mucosal immunization: a review of strategies and challenges","volume":"31","author":"Patel","year":"2014","journal-title":"Crit. Rev. Ther. Drug Carr."},{"key":"10.1016\/j.fsi.2017.03.030_bib13","series-title":"Joint FAO\/WHO (Food and Agriculture Organization\/World Health Organization) Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food","author":"FAO\/WHO","year":"2002"},{"key":"10.1016\/j.fsi.2017.03.030_bib14","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/j.1365-2672.2008.03773.x","article-title":"The safety of Bacillus subtilis and Bacillus indicus as food probiotics","volume":"105","author":"Hong","year":"2008","journal-title":"J.\u00a0Appl. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.030_bib15","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s00216-011-4835-4","article-title":"Bacterial spores as platforms for bioanalytical and biomedical applications","volume":"400","author":"Knecht","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.fsi.2017.03.030_bib16","doi-asserted-by":"crossref","first-page":"4215","DOI":"10.1016\/S0264-410X(03)00492-4","article-title":"Germination of the spore in the gastrointestinal tract provides a novel route for heterologous antigen delivery","volume":"21","author":"Duc","year":"2003","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.fsi.2017.03.030_bib17","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.fsi.2014.02.008","article-title":"Dietary supplementation of Bacillus subtilis and fructooligosaccharide enhance the growth, non-specific immunity of juvenile ovate pompano, trachinotus ovatus and its disease resistance against Vibrio vulnificus","volume":"38","author":"Zhang","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.fsi.2009.09.016","article-title":"Immune responses of Fenneropenaeus chinensis against white spot syndrome virus after oral delivery of VP28 using Bacillus subtilis as vehicles","volume":"28","author":"Fu","year":"2010","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib19","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/jam.12550","article-title":"First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface","volume":"117","author":"Valdez","year":"2014","journal-title":"J.\u00a0Appl. Microbiol."},{"issue":"1","key":"10.1016\/j.fsi.2017.03.030_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-6851(01)00438-8","article-title":"Cysteine proteases of parasitic organisms","volume":"120","author":"Sajid","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.fsi.2017.03.030_bib21","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1007\/s00436-011-2751-3","article-title":"Molecular characterization and expression of a cysteine protease from Clonorchis sinensis and its application for serodiagnosis of clonorchiasis","volume":"110","author":"Lv","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.03.030_bib22","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s00436-013-3627-5","article-title":"Cysteine protease is a major component in the excretory\/secretory products of Euclinostomum heterostomum (digenea: clinostomidae)","volume":"113","author":"Shareef","year":"2014","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.03.030_bib23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3347\/kjp.2012.50.1.45","article-title":"Protective role of purified cysteine proteinases against Fasciola gigantica infection in experimental animals","volume":"50","author":"El-Ahwany","year":"2012","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.fsi.2017.03.030_bib24","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1086\/386346","article-title":"Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms","volume":"189","author":"Loukas","year":"2004","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.fsi.2017.03.030_bib25","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s00436-004-1097-5","article-title":"The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment","volume":"93","author":"Li","year":"2004","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.03.030_bib26","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s00436-006-0242-8","article-title":"Long-term storage of Clonorchis sinensis metacercariae in\u00a0vitro","volume":"100","author":"Li","year":"2006","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.03.030_bib27","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1016\/j.vaccine.2008.02.015","article-title":"Oral administration of a Bacillus subtilis spore-based vaccine expressing Clonorchis sinensis tegumental protein 22.3 kDa confers protection against Clonorchis sinensis","volume":"26","author":"Zhou","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.030_bib28","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1016\/j.vaccine.2014.01.039","article-title":"Surface display of Clonorchis sinensis enolase on Bacillus subtilis spores potentializes an oral vaccine candidate","volume":"32","author":"Wang","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.03.030_bib29","doi-asserted-by":"crossref","first-page":"3095","DOI":"10.1007\/s00436-014-3975-9","article-title":"Systemic and local mucosal immune responses induced by orally delivered Bacillus subtilis spore expressing leucine aminopeptidase 2 of Clonorchis sinensis","volume":"113","author":"Qu","year":"2014","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.fsi.2017.03.030_bib30","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1186\/s13071-016-1928-0","article-title":"The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease","volume":"9","author":"Tang","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.fsi.2017.03.030_bib31","doi-asserted-by":"crossref","first-page":"4952","DOI":"10.1128\/jb.173.16.4952-4958.1991","article-title":"Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases","volume":"173","author":"Wu","year":"1991","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.fsi.2017.03.030_bib32","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.1016\/S0021-9258(18)62213-6","article-title":"The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis","volume":"246","author":"Leighton","year":"1971","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.fsi.2017.03.030_bib33","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.fsi.2014.08.020","article-title":"Adaptive immune responses at mucosal surfaces of teleost fish","volume":"40","author":"Rombout","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib34","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1016\/j.dci.2011.11.009","article-title":"Mucosal immunoglobulins and B cells of teleost fish","volume":"35","author":"Salinas","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib35","first-page":"525","article-title":"The mucosal immune system of teleost fish","volume":"4","author":"Salinas","year":"2015","journal-title":"Biol. (Basel)"},{"key":"10.1016\/j.fsi.2017.03.030_bib36","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.fsi.2010.09.001","article-title":"Teleost intestinal immunology","volume":"31","author":"Rombout","year":"2011","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib37","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/978-3-319-20819-0_9","article-title":"Antibody repertoires in fish","volume":"57","author":"Bengt\u00e9n","year":"2015","journal-title":"Results Probl. Cell Differ."},{"key":"10.1016\/j.fsi.2017.03.030_bib38","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/978-3-319-20819-0_10","article-title":"Unique features of fish immune repertoires: particularities of adaptive immunity within the largest group of vertebrates","volume":"57","author":"Magadan","year":"2015","journal-title":"Results Probl. Cell Differ."},{"key":"10.1016\/j.fsi.2017.03.030_bib39","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.addr.2015.03.007","article-title":"Delivery strategies to enhance oral vaccination against enteric infections","volume":"91","author":"Davitt","year":"2015","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.fsi.2017.03.030_bib40","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1099\/jmm.0.076430-0","article-title":"Expression of Helicobacter pylori urease B on the surface of Bacillus subtilis spores","volume":"64","author":"Zhou","year":"2015","journal-title":"J.\u00a0Med. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.030_bib41","first-page":"235","article-title":"Resistance of Bacillus subtilis spores against gastrointestinal tract environment","volume":"8","author":"Zhou","year":"2008","journal-title":"J.\u00a0Trop."},{"key":"10.1016\/j.fsi.2017.03.030_bib42","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0145-305X(02)00137-4","article-title":"Humoral immune response of carp (Cyprinus carpio) induced by oral immunization with liposome-entrapped antigen","volume":"27","author":"Irie","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.03.030_bib43","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.femsre.2004.12.001","article-title":"The use of bacterial spore formers as probiotics","volume":"29","author":"Hong","year":"2005","journal-title":"FEMS. Microbiol. Rev."},{"key":"10.1016\/j.fsi.2017.03.030_bib44","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1128\/AEM.70.4.2161-2171.2004","article-title":"Characterization of Bacillus probiotics available for human use","volume":"70","author":"Duc","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fsi.2017.03.030_bib45","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.physbeh.2017.01.010","article-title":"Parasite-induced increases in the energy costs of movement of host freshwater fish","volume":"171","author":"Slav\u00edk","year":"2017","journal-title":"Physiol. Behav."},{"key":"10.1016\/j.fsi.2017.03.030_bib46","doi-asserted-by":"crossref","DOI":"10.3390\/md14120232","article-title":"Protective effects of hydrolyzed nucleoproteins from Salmon Milt against ethanol-induced liver injury in rats","volume":"14","author":"Kojima-Yuasa","year":"2016","journal-title":"Mar. Drugs"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301584?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817301584?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:02:59Z","timestamp":1658840579000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817301584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":46,"alternative-id":["S1050464817301584"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.030","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp (Ctenopharyngodon idellus): Induces immune responses and has no damage on liver and intestine function","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.03.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}